[1]石卫红,李小林,巫国辉,等. 瘦素受体（OB-RGRP）水平对脂肪细胞的影响及其作用机制研究[J].中国美容医学,2019,(11):90-93.
　[J].Medical Aesthetics and Beauty,2019,(11):90-93.
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 瘦素受体（OB-RGRP）水平对脂肪细胞的影响及其作用机制研究
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2019年11期
页码:
90-93
栏目:
出版日期:
2019-11-05

文章信息/Info

文章编号:
1008-6455（2019）11-0090-03
作者:
 石卫红; 李小林; 巫国辉; 覃 煦; 万冠群; 曾 琪; 胡云刚
关键词:
 ; [关键词]OB-RGRP; 瘦素受体; Western blot; JAK2/STAT3信号通路; 脂肪细胞; 肥胖症
分类号:
R329.2+8
文献标志码:
A
摘要:
[摘要]目的：为了探讨不同水平的瘦素受体（OB-RGRP）调节大鼠脂肪细胞的研究及其作用机制，明确其对脂肪细胞的影
响，为瘦素受体治疗肥胖症提供理论依据。方法：原代分离大鼠脂肪细胞，利用油红O染色进行鉴定，构建过表达OB-RGRP和
干扰OB-RGRP载体，利用Western blot验证细胞转染效果，并检测JAK2、STAT3及其磷酸化的表达情况。结果：细胞分离正
确，构建的OB-RGRP-siRNA转染有效，与对照组相比，过表达OB-RGRP组的p-JAK2和p-STAT3表达明显升高，干扰OB-RGRP组的
p-JAK2和p-STAT3表达明显下降，差异具有统计学意义（P＜0.05）。而JAK2、STAT3总蛋白各组之间无差异。结论：OB-RGRP
水平通过JAK2/STAT3信号通路在调节瘦素抵抗中起着重要作用，调节脂肪代谢，有助于改善瘦素抵抗及肥胖防治。

Abstract:
 [中图分类号] [文献标志码]A [文章编号]
The Effect of Leptin Receptor(OB-RGRP) on Adipocytes and Its Mechanism
SHI Wei-hong,LI Xiao-lin,WU Guo-hui,QIN Xu,WAN Guan-qun,ZENG Qi,HU Yun-gang
(Department of Plastic Maxillofacial Surgery,Jiangxi Provincial People’s Hospital,Nanchang 330006,Jiangxi,China)
Abstract: Objective To investigate the mechanism of leptin receptors(OB-RGRP) in regulating obesity In order to investigate
the regulation of different levels of leptin receptor on adipocytes in rats and its mechanism of action, and to clarify its effect on
adipocytes, so as to provide theoretical basis for the treatment of obesity by leptin receptor. Methods Primary rat adipocytes
were isolated and identified by oil red O staining. OB-RGRP overexpressed and OB-RGRP interfering vectors were constructed.
The cell transfection effect was verified by Western blot, and the expression of JAK2, STAT3 and their phosphorylation were
detected. Results The cells were isolated correctly and the constructed OB-RGRP-siRNA was effective in transfection.
Compared with the control group, the overexpression of p-JAK2 and p-STAT3 in the OB-RGRP group was significantly
increased, while the expression of p-JAK2 and p-STAT3 in the OB-RGRP group was significantly decreased, and the difference
was statistically significant (P ＜0.05).There was no difference between JAK2 and STAT3 groups. Conclusion OB-RGRP level
plays an important role in regulating leptin resistance through the JAK2/STAT3 signaling pathway, regulating fat metabolism
and contributing to the improvement of leptin resistance and the prevention and treatment of obesity.
Key words: OB-RGRP; leptin receptor; Western blot; JAK2/STAT3 signal path; adipocyte; adiposis

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更新日期/Last Update: 2019-12-09