[1]徐 斌,刘 毅. 不同分离方法及培养条件对人脐带间充质干细胞生物活性的影响[J].中国美容医学,2020,(2):71-73.
　XU Bin,LIU Yi. Effects of Separation Methods and Culture Conditions on Biological Characteristicsof Human Umbilical Cord-mesenchymal Stem Cells[J].Medical Aesthetics and Beauty,2020,(2):71-73.
点击复制

 不同分离方法及培养条件对人脐带间充质干细胞生物活性的影响
()
分享到：

《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2020年2期
页码:
71-73
栏目:
出版日期:
2020-03-15

文章信息/Info

Title:
 Effects of Separation Methods and Culture Conditions on Biological Characteristics
of Human Umbilical Cord-mesenchymal Stem Cells
文章编号:
1008-6455（2020）02-0071-03
作者:
 徐 斌1; 刘 毅2
Author(s):
 XU Bin1; LIU Yi2
关键词:
 ; [关键词]组织块贴壁法; 酶消化法; 人脐带间充质干细胞; 培养基; 生物活性
Keywords:
 ; Key words: tissue adherence method;  collagenase digestion method;  human umbilical cord-mesenchymal stemcells(hUCMSCs);  culture medium;  biological characteristics
分类号:
Q813.1
文献标志码:
A
摘要:
[摘要]目的：观察不同分离方法及培养条件对人脐带间充质干细胞(Human umbilical cord-mesenchymal stem cells，
hUCMSCs)生物活性的影响。方法：分别用组织块贴壁法、酶消化法获取hUCMSCs，进行原代培养，记录各组首次传代时间，
相差显微镜观察细胞的形态，流式细胞仪鉴定其CD29、CD44、HLA-ABC、CD34、CD45、HLA-DR抗原表达。分别用DMEM-LG、
DMEM-HG和DMEM-F12 3种培养基培养第3代、第7代细胞，MTT法比较不同时间点3种培养基培养细胞的生长情况。结果：两
种分离方法均能得到较均一的梭形或者多角形贴壁细胞，胞核大胞浆丰富，CD29、CD44、HLA-ABC抗原表达阳性，CD34、
CD45、HLA-DR抗原表达阴性。用DMEM-F12培养的细胞活力更好，细胞生长更迅速。结论：两种分离方法均能得到hUCMSCs，
DMEM-F12培养基更能够促进细胞的增殖，较适合于培养hUCMSCs。

Abstract:
Abstract: Objective To observe the effects of different separation methods and culture conditions on biological characteristics
of hUCMSCs. Methods The hUCMSCs were isolated form human umbilical cord by tissue adherence and digested with
collagenase, the time of passage was recorded, in addition, the cell morphology was observed by phase contrast microscope,
and the CD29、CD44、HLA-ABC、CD34、CD45、HLA-DR antigen expression was identifi ed using fl ow cytometry. The
3rd and the 7th generation cells were cultured with DMEM-LG, MEM-HG, and DMEM-F12 culture medium. MTT was used to
evaluate the growth of hUCMSCs. Phase contrast microscope was used to observe the morphological changes of aging cells.
Results hUCMSCs could be separated by each method. The adherent cells showed shuttle or multiple angle shapes, with rich
cytoplasm, and positive for CD29、CD44、 HLA-ABC antigen, negative for CD34、CD45 and HLA-DR. DMEM-F12 could
promote the proliferation of quiescent cells. And the cells presented the better viability. Conclusion Both of the two methods
can produce hUCMSCs, DMEM-F12 medium is more suitable for the culture of hUCMSCs.

相似文献/References:

[1]. 埋线美容专栏[J].中国美容医学,2018,(01):0.
　[J].Medical Aesthetics and Beauty,2018,(2):0.
[2]杨 震. 应用透明质酸颏部填充联合A型肉毒毒素咬肌注射重塑面部下1/3轮廓的效果观察[J].中国美容医学,2018,(03):1.
　YANG Zhen. Effect of Hyaluronic Acid Chin Filling Combined with Botulinum Toxin Type A Masseter Muscle Injection to Reconstruct the Facial Contour of the Lower 1/3[J].Medical Aesthetics and Beauty,2018,(2):1.
[3]祝闽辉,张栋梁,叶祥柏,等. 皮肤软组织扩张术埋置注液期并发症危险因素研究[J].中国美容医学,2018,(03):4.
　ZHU Min-hui,ZHANG Dong-liang,YE Xiang-bo,et al. Risk Factors of Complications after Tissue Expasion[J].Medical Aesthetics and Beauty,2018,(2):4.
[4]刘申松,杨永成. 自体脂肪颗粒移植联合假体隆乳术在哺乳后乳腺萎缩整形术中的应用[J].中国美容医学,2018,(03):9.
　LIU Shen-song,YANG Yong-cheng. Application of Autologous Fat Granule Graft Combined with Prosthesis Combined Augmentation Mammoplasty in Breast Reconstruction of Breast Atrophy Patients after Breast-feeding[J].Medical Aesthetics and Beauty,2018,(2):9.
[5]韩 星,胡守舵,陈晓芳,等.自体脂肪颗粒移植促进手部年轻化的临床观察[J].中国美容医学,2018,(03):12.
　HAN Xing,HU Shou-duo,CHEN Xiao-fang,et al. The Effect of Autologous Fat Grafting in Hand Rejuvenation[J].Medical Aesthetics and Beauty,2018,(2):12.
[6]李胜旭,苏映军,杨 宽,等. 封闭负压引流技术在先天性并指分指植皮术中的应用[J].中国美容医学,2018,(03):15.
　LI Sheng-xu,SU Ying-jun,YANG Kuan,et al. Application of Vacuum Sealing Drainage for Skin Graft in Patients with Congenital Syndactyly of the Hand[J].Medical Aesthetics and Beauty,2018,(2):15.
[7]陈 波,路 会,马 力,等. 钝针皮下层扇形注射透明质酸矫正面颊部凹陷[J].中国美容医学,2018,(03):18.
　CHEN Bo,LU Hui,MA Li,et al. Correction of Cheek Depression by Fanshaped Hyaluronic Acid Injection in Subcutaneous Layers with Blunt Needle[J].Medical Aesthetics and Beauty,2018,(2):18.
[8]曾德斌,栗鹏程,邱勋永,等. 不同超薄游离穿支皮瓣修复手部软组织缺损效果比较[J].中国美容医学,2018,(03):21.
　ZENG De-bin,LI Peng-cheng,QIU Xun-yong,et al. Comparison on the Repair Effect of Different Ultrathin Free Perforator Flaps in the Treatment of Patients with Soft Tissue Defect of Hand[J].Medical Aesthetics and Beauty,2018,(2):21.
[9]程 定,崔树英,郑 乐,等. 改道引流联合VSD技术修复会阴部撕裂伤的临床研究[J].中国美容医学,2018,(03):24.
　CHENG Ding,CUI Shu-ying,ZHENG Le,et al. Clinical Study on Diverted Drainage Combined with Vacuum Sealing Drainage Technique to Repair the Laceration of the Perineum[J].Medical Aesthetics and Beauty,2018,(2):24.
[10]李云峰,吴泽勇,黄海华,等. 全厚皮片游离移植矫治儿童足背瘢痕挛缩畸形[J].中国美容医学,2018,(03):27.
　LI Yun-feng,WU Ze-yong,HUANG Hai-hua,et al. Full-thickness Skin Graft for the Treatment of Cicatricial Contracture of the Dorsum of Foot in Children[J].Medical Aesthetics and Beauty,2018,(2):27.

更新日期/Last Update: 2020-03-31