[1] 王 静,魏 霞,张 岚,等. 甲壳胺膜负压伤口疗法对难愈性创面血管内皮生长因子和成纤维细胞的影响[J].中国美容医学,2020,(9):92-95.
　 WANG Jing,WEI Xia,ZHANG Lan,et al. The Influence of the Treatment to Refractory Wounds by Chitosan MembraneNegative Pressure Wound Therapy on Vascular Endothelial Growth Factor andFibroblast[J].Medical Aesthetics and Beauty,2020,(9):92-95.
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 甲壳胺膜负压伤口疗法对难愈性创面血管内皮生长因子和成纤维细胞的影响
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2020年9期
页码:
92-95
栏目:
出版日期:
2020-09-10

文章信息/Info

Title:
 The Influence of the Treatment to Refractory Wounds by Chitosan Membrane
Negative Pressure Wound Therapy on Vascular Endothelial Growth Factor and
Fibroblast
文章编号:
1008-6455（2020）09-0092-03
作者:
 王 静; 魏 霞; 张 岚; 徐炜志; 葛秀峰; 狄青海; 张家诚; 薛 峰; 逄丽华; 宗 纲
Author(s):
 WANG Jing; WEI Xia; ZHANG Lan; XU Wei-zhi; GE Xiu-feng; DI Qing-hai; ZHANG Jia-cheng; XUEFeng; PANG Li-hua; ZONG Gang
关键词:
 [关键词]负压伤口疗法; 甲壳胺膜; 难愈性创面; 血管内皮生长因子; 成纤维细胞
Keywords:
 Key words: negative pressure wound therapy(NPWT);  chitosan membrane;  refractory wounds;  vascular endothelial growthfactor(VEGF);  fibroblast
分类号:
R622
文献标志码:
A
摘要:
[摘要]目的：观察甲壳胺膜负压伤口疗法治疗难愈创面对其新生肉芽组织中血管内皮生长因子和成纤维细胞表达的影响。方
法：2017年11月-2019年11月，笔者科室收治的100例难愈创面患者按随机数字表法分为治疗组（甲壳胺膜负压伤口疗法治
疗）和对照组（传统外科换药治疗），每组50例。患者入院后当日、治疗第7天、第14天分别留取创面中心肉芽组织标本送
免疫组化检测，电子显微镜下观察肉芽组织中血管内皮生长因子（Vascular endothelial growth factor，VEGF）和成纤维
细胞（Fibroblast）的表达情况，测算每组患者治疗后第7天和第14天VEGF和成纤维细胞分布密集和稀疏的情况。结果：入
院当日，两组患者创面肉芽组织的VEGF和成纤维细胞均表达稀疏；第7天，治疗组患者中有66%的难愈创面肉芽组织的VEGF和
成纤维细胞表达密集；第14天，治疗组患者中有69%的难愈创面肉芽组织的VEGF和成纤维细胞表达密集。而对照组在治疗第7
天、第14天全部表达为稀疏。结论：甲壳胺膜负压伤口疗法可以使大部分难愈创面肉芽组织中成纤维细胞和血管内皮生长因
子表达密集，从细胞蛋白水平证明该治疗方法可以有效促进难愈创面新生肉芽组织的生长。

Abstract:
Abstract: Objective To explore the influence of chitosan membrane negative pressure wound therapy (NPWT) to the
expressions of vascular endothelial growth factor (VEGF)and fibroblasts in the granulation tissues of refractory wounds.
Methods From November 2017 to November 2019, 100 cases of refractory wounds hospitalized in our department were
randomly divided into two groups: the treatment group (50 cases, treated with chitosan membrane NPWT) and the control group
(50 cases, treated with traditional dressing-change method). To keep the samples of granulation tissue at the center of the wound
and send them to immunohistochemical detection on the day after the patient was admitted, on the 7th and 14th days of treatment.
The expression of VEGF and fibroblasts in granulation tissue was observed under an electron microscope. Measure the dense
and sparse distribution of VEGF and fibroblasts on the 7th and 14th days after treatment in each group of patients. Results On
the day of admission, VEGF and fibroblasts in the granulation tissue of the two groups of patients were sparsely expressed.
On the 7th days of treatment, VEGF and fibroblasts expression in granulation tissue of refractory wounds was dense in 66% of
patients. On the 14th days of treatment, VEGF and fibroblasts expression in granulation tissue of refractory wounds was dense in
69% of treatment group. But the control group expressed sparsely on the 7th and 14th days of treatment. Conclusion Chitosan
membrane NPWT can make VEGF and fibroblasts expression expressed densely in granulation tissue of refractory wounds.
It is proved from the cellular protein level that the treatment can effectively promote the growth of new granulation tissue in
refractory wounds.
更新日期/Last Update: 2020-09-25