[1] 白坤宏,向 婷,赵敏越,等. 炎症微环境下低强度高频振动对牙周膜干细胞成骨分化能力的影响[J].中国美容医学,2021,(2):96-91.
　 BAI Kun-hong,XIANG Ting,ZHAO Min-yue,et al. Effect of LMHFV on Osteogenic Differentiation of Periodontal Ligament Stem Cells inInflammation Microenvironment[J].Medical Aesthetics and Beauty,2021,(2):96-91.
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 炎症微环境下低强度高频振动对牙周膜干细胞成骨分化能力的影响
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2021年2期
页码:
96-91
栏目:
出版日期:
2021-02-10

文章信息/Info

Title:
 Effect of LMHFV on Osteogenic Differentiation of Periodontal Ligament Stem Cells in
Inflammation Microenvironment
文章编号:
1008-6455（2021）02-0086-05
作者:
 白坤宏1; 向 婷2; 赵敏越2; 卜雯卿2; 程 政1; 王 菲2
Author(s):
 BAI Kun-hong1; XIANG Ting2; ZHAO Min-yue2; BU Wen-qing2; CHENG Zheng1; WANG Fei2
关键词:
 [关键词]炎症环境; 振动; 牙周膜干细胞; 成骨分化; 牙周病; 蛋白水平
Keywords:
 Key words: inflammatory environment;  vibration;  periodontal ligament stem cells;  osteogenic differentiation;  periodontaldisease;  protein level
分类号:
R781.4
文献标志码:
A
摘要:
[摘要]目的：研究在炎症微环境下低强度高频振动（Low-magnitude high frequency vibration，LMHFV）对牙周膜干细胞
（Periodontal ligament stem cells，PDLSCs）成骨分化能力的影响。方法：体外分离培养PDLSCs；流式细胞术检测间充
质干细胞表面标志物；构建体外炎症微环境：通过向常规培养基（含10% FBS，α-MEM培养基）中添加TNF-α（10ng/ml）
与IL-1β（5ng/ml）模拟炎症微环境；加载LMHFV（加速度=0.3g、频率=40Hz，时间=15min/24h）刺激；通过Realtime RTPCR
、Western-blot检测成骨相关基因、蛋白表达水平；通过茜素红染色检测细胞成骨分化能力。采用SPSS 21.0软件对数据进
行统计学分析。结果：在炎症微环境下，LMHFV刺激后PDLSCs的RUNX-2、ALP、COL-1基因表达量明显升高，ALP、COL-1、OCN的
蛋白表达量明显升高，但相较常规培养的PDLSCs，其成骨基因与蛋白表达量仍有明显降低。结论：炎症微环境下，LMHFV刺
激可以一定程度上提高PDLSCs的成骨分化能力，但是并不能完全抵消炎性条件对PDLSCs成骨能力的抑制作用。
Abstract:
Abstract: Objective To investigate the effect of low magnitude high frequency vibration (LMHFV) on osteogenic
differentiation of periodontal ligament stem cells (PDLSCs) in an inflammatory microenvironment. Methods PDLSCs were
isolated from premolar teeth and mesenchymal stem cell surface markers were analyzed by flow cytometry. Construction of
inflammatory microenvironment in vitro: the inflammatory microenvironment was simulated by adding TNF-α(10ng/ml) and
IL-1β(5ng/ml) to the conventional medium (containing 10% FBS, α-MEM culture medium). Loading LMHFV stimulation
(acceleration=0.3g, frequency=40Hz, time=15min/24h). The expression of osteogenesis gene was analyzed by Realtime RTPCR,
and the expression of osteogenesis protein was analyzed by Western-blot. The capability of osteogenesis differentiation
was evaluated by alizarin red staining. SPSS 21.0 software was used to analyze the data. Results In the inflammatory
microenvironment, the gene expressions of Runx-2, ALP, COL-1 and the protein expressions of ALP, COL-1 and OCN in
PDLSCs stimulated by LMHFV were significantly increased. However, compared with the conventional cultured PDLSCs, the
osteogenic gene and protein expressions were still significantly decreased. Conclusion LMHFV could enhance osteogenic
differentiation of PDLSCs in inflammatory microenvironment.However, it can not completely counteract the inhibitory effect of
inflammatory microenvironment on the osteogenic differentiation ability of PDLSCs.
更新日期/Last Update: 2021-03-09