[1]杨 靖,谢 群.原花青素对瘢痕疙瘩成纤维细胞增殖、迁移及TLR4/MyD88通路的影响?/html>[J].中国美容医学,2021,(11):85-89.
　YANG Jing,XIE Qun.Effect of Procyanidins on the Proliferation,Migration and Tlr4/Myd88 Pathway of Keloid Fibroblasts?/html>[J].Medical Aesthetics and Beauty,2021,(11):85-89.
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原花青素对瘢痕疙瘩成纤维细胞增殖、迁移及TLR4/MyD88通路的影响?/html>()
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2021年11期
页码:
85-89
栏目:
出版日期:
2021-11-10

文章信息/Info

Title:
Effect of Procyanidins on the Proliferation,Migration and Tlr4/Myd88 Pathway of Keloid Fibroblasts?/html>
文章编号:
1008-6455（2021）11-0085-05
作者:
杨 靖; 谢 群
Author(s):
YANG Jing; XIE Qun
关键词:
Keywords:
Key words: procyanidins;  keloid fi broblasts;  cell proliferation;  cell migration;  TLR4/MyD88 pathway
分类号:
]R619+ .6
文献标志码:
A
摘要:
[摘要]目的：探讨原花青素对瘢痕疙瘩成纤维细胞（KFs）增殖、迁移及TLR4/MyD88通路的影响。方法：体外培养96孔 （5×103 个/孔）KFs细胞，用MTT法确定原花青素的半数抑制浓度；96孔（5×103 个/孔）KFs细胞随机分为对照组、原花青 素低剂量组、原花青素中剂量组和原花青素高剂量组,每组24孔（5×103 个/孔）。对照组细胞不进行处理；原花青素低、 中、高剂量组分别用终浓度为25、50、100mg/L的原花青素干预KFs细胞。24h后分别采用MTT法、流式细胞术和细胞划痕法 检测KFs细胞增殖率、凋亡率和迁移能力；酶联免疫吸附法检测KFs细胞中IL-23、IL-17A和IL-6水平；实时荧光PCR和 Western Blot法检测KFs细胞中TLR4、MyD88和NF-κB mRNA及蛋白水平。结果：随着原花青素浓度增加，KFs细胞增殖率 降低，50mg/L为半数抑制浓度。与对照组比较，其余各组细胞迁移距离降低，细胞凋亡率增加，细胞中IL-23、IL-17A和 IL-6，TLR4、MyD88和NF-κB mRNA及蛋白水平降低，且随着原花青素剂量增加，各原花青素剂量组各指标变化呈剂量反应 关系，差异有统计学意义（P＜0.05）。结论：原花青素能抑制KFs细胞增殖和迁移，促进KFs细胞凋亡，其机制可能与抑 制TLR4/MyD88通路的激活有关。
Abstract:
Abstract: Objective To investigate the effect of procyanidins on the proliferation, migration and TLR4/MyD88 pathway of keloid fibroblasts. Methods Culture 96-well (5×103 cells/well) KFs cells in vitro, and determine the half inhibitory concentration of proanthocyanidin by MTT method; 96-well (5×103 cells/well) KFs cells were randomly divided into control group, low-dose proanthocyanidin group, and proanthocyanidin medium The dose group and the high-dose proanthocyanidin group, each group has 24 holes (5×103 pieces/hole). The control group cells were not treated; the proanthocyanidin low, medium, and high-dose groups were treated with proanthocyanidins at fi nal concentrations of 25, 50, and 100 mg/L respectivelto interfere with KFs cells. After 24 hours, MTT method, flow cytometry and cell scratch method were used to detect the proliferation rate, apoptosis rate and migration ability of KFs cells, enzyme-linked immunosorbent assay was used to detect the levels of IL-23, IL-17A and IL-6 in KFs cells. Real-time fl uorescent PCR and Western Blot method to detect TLR4, MyD88 and NF-κB mRNA and protein levels in KFs cells. Results As the concentration of proanthocyanidins increases, the proliferation rate of KFs cells decreases, and 50 mg/L is the half inhibitory concentration. Compared with the control group, the cell migration distance of the other groups decreased, and the apoptosis rate increased. The mRNA and protein levels of IL-23, IL17A, IL-6, TLR4, MyD88, and NF-κB in the cells decreased, and with procyanidins With the increase of the dose, the changes in the indicators of each proanthocyanidin dose group showed a dose-response relationship, and the diff erence was statistically significant (P＜0.05). Conclusion Procyanidins can inhibit the proliferation and migration of KFs cells and promote the apoptosis of KFs cells. The mechanism may be related to the inhibition of the activation of the TLR4/MyD88 pathway.

相似文献/References:

[1]梁 博,李国东,牟江月,等.补骨脂异黄酮通过miR-497-5p调控对瘢痕疙瘩成纤维细胞增殖和迁移的机制研究[J].中国美容医学,2024,(6):60.
　LIANG Bo,LI Guodong,MOU Jiangyue,et al.Mechanism Study on Proliferation and Migration of Keloid Fibroblasts Regulated by Psoralen Isofl avones Via miR-497-5p[J].Medical Aesthetics and Beauty,2024,(11):60.

更新日期/Last Update: 2021-12-02