[1]段 强,冯群燕.葛根素对人皮肤鳞状细胞癌中PI3K/AKT信号通路激活状态的干预作用[J].中国美容医学,2022,(4):105-109.
　DUAN Qiang,FENG Qunyan.Activation Status of PI3K/AKT Signaling Pathway in Human Cutaneous Squamous Cell Carcinoma and the Intervention Effect of Puerarin[J].Medical Aesthetics and Beauty,2022,(4):105-109.
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葛根素对人皮肤鳞状细胞癌中PI3K/AKT信号通路激活状态的干预作用()
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2022年4期
页码:
105-109
栏目:
出版日期:
2022-04-10

文章信息/Info

Title:
Activation Status of PI3K/AKT Signaling Pathway in Human Cutaneous Squamous Cell Carcinoma and the Intervention Effect of Puerarin
文章编号:
1008-6455（2022）04-0105-04
作者:
段 强; 冯群燕
(咸阳市中心医院皮肤科 陕西 咸阳 712000 )
Author(s):
DUAN Qiang; FENG Qunyan
(Department of Dermatology,Xianyang Central Hospital,Xianyang 712000,Shaanxi,China)
关键词:
皮肤鳞状细胞癌; 葛根素; 增殖; 侵袭; PI3K/AKT信号通路
Keywords:
cutaneous squamous cell carcinoma;  puerarin;  proliferation;  invasion;  PI3K/AKT signaling pathway
分类号:
R739.5
文献标志码:
A [
摘要:
目的：研究葛根素对人皮肤鳞状细胞癌（CSCC）中磷脂酰肌醇-3激酶（PI3K）/蛋白激酶B（AKT）信号通路激活状态的干预作用。方法：收集人CSCC组织（52例）及正常皮肤组织（35例），培养人CSCC细胞株SCL-1、A431、HSC-5和人永生化角质形成细胞株HaCaT细胞，检测p-PI3K、p-AKT的表达水平；SCL-1细胞进行分组，用不同剂量葛根素（20μmol/L、40μmol/L、80μmol/L）处理，转染阴性对照（NC）质粒或PI3K质粒，检测细胞增殖活力、侵袭数目及p-PI3K、p-AKT的表达水平；BALB/c-nu裸鼠皮下注射SCL-1细胞建立移植瘤模型，100mg/kg葛根素灌胃处理3周后检测移植瘤质量、体积及p-PI3K、p-AKT的表达水平。结果：人CSCC组织中p-PI3K、p-AKT的表达水平高于正常皮肤组织（P＜0.05）；人CSCC细胞株中p-PI3K、p-AKT的表达水平高于HaCaT细胞且SCL-1中p-PI3K、p-AKT表达上调最显著（P＜0.05）；不同剂量葛根素处理后，SCL-1细胞的增殖活力、侵袭数目及p-PI3K、p-AKT的表达水平均降低（均P＜0.05）；转染质粒后，PI3K质粒+80μmol/L葛根素组SCL-1细胞的增殖活力、侵袭数目高于NC质粒+80μmol/L葛根素组（P＜0.05）；葛根素组裸鼠的移植瘤质量、体积及p-PI3K、p-AKT的表达水平均低于对照组（P＜0.05）。结论：PI3K/AKT信号通路过度激活与CSCC发病有关，葛根素通过抑制该通路发挥抗CSCC作用。
Abstract:
Objective To study the intervention effect of puerarin on the activation status of phosphatidylinositol-3 kinase (PI3K) / protein kinase B (AKT) signaling pathway in human cutaneous squamous cell carcinoma(CSCC). Methods human CSCCtissues (52 cases)and normal skin tissues (35 cases) were collected, human CSCC cell lines SCL-1, A431, HSC-5 and human immortalized keratinocyte line HaCaT were cultured. Then the expression levels of p-PI3K and p-AKT were detected. SCL-1 cells were divided into groups, treated with different doses of Puerarin (20 μmol/L, 40 μmol/L, 80 μmol/L), and transfected with negative control (NC) plasmid or PI3K plasmid. Then the proliferation viability, invaded numbers, the expression levels of p-PI3K and p-AKT were detected. The transplanted tumor model was established by subcutaneous injection of SCL-1 cells into BALB /c-nu nude mice, 100 mg/kg puerarin was given by gavage for 3 weeks and the weight and volume of transplanted tumor, the expression levels of p-PI3K and p-AKT were detected. Results The expression levels of p-PI3K and p-AKT in human CSCC tissue were higher than those in normal skin tissue (P ＜0.05), the expression levels of p-PI3K and p-AKT in human CSCC cell line were higher than those in HaCaT cell line (P ＜0.05), and the expression levels of p-PI3K and p-AKT in SCL-1 were up-regulated most significantly(P＜0.05). After different doses of puerarin treatment, the proliferation viability, invaded number and the expression levels of p-PI3K and p-AKT in SCL-1 cells decreased (all P ＜0.05).After plasmid transfection, the proliferation viability, invaded number of PI3K plasmid+80 μmol/L puerarin group were higher than those in NC plasmid+80 μmol/L puerarin group(P ＜0.05). The weight and volume of transplanted tumor and the expression levels of p-PI3K and p-AKT in puerarin group were lower than those in control group (P<0.05). Conclusion The overactivation of PI3K/AKT signaling pathway relates to the pathogenesis of CSCC. Puerarin exerts its anti CSCC effect by inhibiting this pathway.

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更新日期/Last Update: 2022-05-06