[1]门 贝,朱 燕.IL-6抑制剂对种植体周围炎小鼠模型的疗效及其作用机制探讨[J].中国美容医学,2022,(5):90-93.
MEN Bei,ZHU Yan.Curative Effect and Mechanism of IL-6 Inhibitor on Mice with Peri-implantitis[J].Medical Aesthetics and Beauty,2022,(5):90-93.
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IL-6抑制剂对种植体周围炎小鼠模型的疗效及其作用机制探讨(
)
《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]
- 卷:
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- 期数:
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2022年5期
- 页码:
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90-93
- 栏目:
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- 出版日期:
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2022-05-10
文章信息/Info
- Title:
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Curative Effect and Mechanism of IL-6 Inhibitor on Mice with Peri-implantitis
- 文章编号:
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1008-6455（2022）05-0090-04
- 作者:
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门 贝1; 朱 燕2
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(1.大众口腔医院口腔修复种植科 湖北 武汉 430000 ;2.湖北省人民医院口腔修复种植科 湖北 武汉 430060 )
- Author(s):
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MEN Bei1; ZHU Yan2
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(1.Department of Prosthodontics and Implant,Dazhong Stomatological Hospital,Wuhan 430000,Hubei,China;2.Department of Prosthodontics and Implant,Hubei Provincial People’s Hospital,Wuhan 430060,Hubei,China)
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- 关键词:
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IL-6抑制剂; 种植体周围炎; 小鼠模型; TNF-α; NF-κB通路; 骨流失
- Keywords:
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IL-6 inhibitor; peri-implantitis; mice model; TNF-; NF-B pathway; bone loss
- 分类号:
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R782.12
- 文献标志码:
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A
- 摘要:
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目的：探讨IL-6抑制剂对种植体周围炎小鼠模型的疗效及其作用机制。方法：选取4周龄雄性小鼠（C57BL/6J）15只，实验总共分为三组（每组5只）：对照组（植入种植体，但未进行结扎诱导）、模型组（结扎诱导，构建种植体周围炎小鼠模型）和抑制剂组（结扎诱导+腹腔注射IL-6抑制剂）。使用微计算机断层扫描（Micro-CT）检测种植体周围骨丢失，苏木精和伊红（HE）染色检测种植体周围牙龈组织的炎症浸润，使用RT-qPCR检测牙龈组织中白介素-6（IL-6）和肿瘤坏死因子-α（TNF-α） mRNA 相对表达量，使用Western blot法检测牙龈组织中NF-κB通路相关蛋白p-P65和p-IκBα蛋白表达水平。结果：Micro-CT结果显示IL-6抑制剂可减少种植体周围炎的骨丧失。HE结果显示IL-6抑制剂处理后，小鼠炎症浸润显著减少。RT-qPCR和Western blot结果显示，IL-6抑制剂可显著降低牙龈组织中IL-6、TNF-α mRNA 相对表达量以及p-P65和p-IκBα的蛋白表达水平。结论：IL-6抑制剂可抑制种植体周围炎的骨丧失和炎症浸润，这些治疗效果可能与抑制TNF-α的表达和NF-κB信号通路有关。
- Abstract:
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Objective To investigate the curative effect and mechanism of IL-6 inhibitor on mice with peri-implantitis. Methods Fifteen 4-week-old male mice (C57BL/6J) were selected, and the experiment was divided into 3 groups (5 mice in each group: the control group (implants implanted, but no ligation induction), the model group (ligation induction: construction peri-implantitis mouse model) and the inhibitor group (ligation induction + intraperitoneal injection of IL-6 inhibitor). Microcomputed tomography (Micro-CT) was used to detect peri-implant bone loss. Hematoxylin and eosin (HE) staining was used to detect inflammatory infiltration in peri-implant gingival tissue. The mRNA relative expression levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF- α) in gingival tissue were detected by RT-qPCR. The protein expression levels of NF-κB pathway related proteins p-P65 and p-IκBα in gingival tissue were detected by Western blot. Results Micro-CT results showed that IL-6 inhibitor could reduce bone loss in peri-implant inflammation. HE results showed that inflammatory infiltration in mice was significantly reduced after IL-6 inhibitor treatment. RT-qPCR and Western blot results showed that IL-6 inhibitor significantly decreased the mRNA relative expression levels of IL-6 and TNF- α, and the protein expression levels of p-P65 and p-IκBα in gingival tissue. Conclusion IL-6 inhibitors can inhibit bone loss and inflammatory infiltration in peri-implantitis, and these therapeutic effects may be related to the inhibition of TNF- α expression and NF- κB signaling pathway
更新日期/Last Update:
2022-05-31