[1]吴 运,吴 群,石梦琪.纯钛表面微纳米形貌对MC3T3-E1细胞的调控作用及机制研究[J].中国美容医学,2022,(6):78-83.
　WU Yun,WU Qun,SHI Mengqi.Regulatory Effect and Mechanism of Titanium Surface Micro-nano Topography on Adhesion, Migration and Osteogenic Differentiation of MC3T3-E1 Cells[J].Medical Aesthetics and Beauty,2022,(6):78-83.
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纯钛表面微纳米形貌对MC3T3-E1细胞的调控作用及机制研究()
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2022年6期
页码:
78-83
栏目:
出版日期:
2022-06-10

文章信息/Info

Title:
Regulatory Effect and Mechanism of Titanium Surface Micro-nano Topography on Adhesion, Migration and Osteogenic Differentiation of MC3T3-E1 Cells
文章编号:
1008-6455（2022）06-0078-06
作者:
吴 运; 吴 群; 石梦琪
(中国人民解放军海军特色医学中心口腔科 上海 200050)
Author(s):
WU Yun; WU Qun; SHI Mengqi
(Department of Stomatology,Naval Medical Center of PLA,Shanghai 200050,China)
关键词:
微纳米形貌; 纯钛表面改性; 整合素α2; 黏附; 迁移; 成骨分化; 信号通路
Keywords:
micro-nano topography;  surface modification of pure titanium;  integrin 2;  adhesion;  migration;  osteogenic differentiation;  signal pathway
分类号:
R783.1
文献标志码:
A
摘要:
目的：研究纯钛表面微纳米形貌对成骨前体细胞MC3T3-E1黏附、迁移及成骨分化的调控作用及机制。方法：纯钛表面抛光作为对照、接种MC3T3-E1细胞作为对照组；采用酸蚀+20 V电压阳极氧化的方法在纯钛表面制备微纳米形貌、接种MC3T3-E1细胞作为微纳米形貌组，转染阴性对照（NC）-siRNA或整合素α2-siRNA作为微纳米形貌+si-NC组、微纳米形貌+si-整合素α2组，给予二甲基亚砜（DMSO）或细胞外调节蛋白激酶（ERK）抑制剂PD98058作为微纳米形貌+DMSO组、微纳米形貌+PD98059组，检测黏附数目、迁移率、成骨诱导分化后OD540及整合素α2、p-ERK、Runx2的表达水平。结果：电镜下观察，酸蚀+20 V电压阳极氧化制备得到管径100 nm的微纳米形貌。微纳米形貌组的黏附数目、迁移率、OD540及整合素α2、p-ERK、Runx2的表达水平均高于对照组，差异有统计学意义（P＜0.05）。微纳米形貌+si-整合素α2组的黏附数目、 迁移率、OD540及整合素α2、p-ERK、Runx2的表达水平均低于微纳米形貌+si-NC组，差异有统计学意义（P＜0.05）。微纳米形貌+PD98059组的黏附数目、迁移率、OD540及p-ERK、Runx2的表达水平均低于微纳米形貌+DMSO组，差异有统计学意义（P＜0.05）。结论：纯钛表面微纳米形貌显著促进MC3T3-E1细胞黏附、迁移及成骨分化，这一促进作用与激活整合素α2/ERK/Runx2通路有关。
Abstract:
Objective To investigate the effect and mechanism of titanium surface micro-nano topography on the adhesion, migration and osteogenic diff erentiation of MC3T3-E1 cells. Methods The pure titanium surface was polished for control, and MC3T3-E1 cells were inoculated as the control group. The micro-nano topography on the pure titanium surface was prepared by acid etching+20 V voltage anodization method, and MC3T3-E1 cells were inoculated as the micro-nano topography group. The negative control (NC)-siRNA or integrin α2-siRNA was transfected as the micro-nano topography+si-NC group, micro-nano topography +si-integrin α2 group. And DMSO or PD98058 was given as micro-nano topography+DMSO group, micro-nano topography+PD98059 group. The adhesion number, migration rate, OD540 after osteogenic differentiation and the expression level of integrin α2, p-ERK, Runx2 were detected. Results Under the electron microscope, the micro-nano topography with diameter of 100 nm was prepared by acid etching+20 V anodizing. The adhesion number, migration rate, OD540 and the expression levels of integrin α2, p-ERK and Runx2 in the micro-nano topography group were higher than those in the control group, the diff erences were statistically signifi cant (P＜0.05). The adhesion number, migration rate, OD540 and the expression levels of integrin α2, p-ERK and Runx2 in the micro-nano topography+si-integrin α2 group were lower than those in the micro-nano topography+siNC group (P＜0.05). The adhesion number, migration rate, OD540 and the expression levels of p-ERK and Runx2 in the micro-nano topography+PD98059 group were lower than those in the micro-nano topography+DMSO group(P＜0.05). Conclusion The titanium surface micro-nano topography signifi cantly promote the adhesion, migration and osteogenic diff erentiation of MC3T3-E1 cells, which is related to the activation of integrin α2/ERK/Runx2 pathway

备注/Memo

备注/Memo:
基金项目：国家自然科学基金青年科学基金项目（名称：微纳米形貌通过影响细胞的机械记忆调控细胞成骨分化的研究，编号：31800804）
更新日期/Last Update: 2022-07-04