[1]任润健,方 磊,贾传龙,等. PDGF-BB对UVB诱导的光老化成纤维细胞中ROS的影响[J].中国美容医学,2018,(05):109-111.
　REN Run-jian,FANG Lei,JIA Chuan-long,et al. Effect of PDGF-BB on Reactive Oxygen Species in UVB-induced Skin Fibroblasts[J].Medical Aesthetics and Beauty,2018,(05):109-111.
点击复制

 PDGF-BB对UVB诱导的光老化成纤维细胞中ROS的影响
()
分享到：

《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2018年05期
页码:
109-111
栏目:
出版日期:
2018-06-10

文章信息/Info

Title:
 Effect of PDGF-BB on Reactive Oxygen Species in UVB-induced Skin Fibroblasts

文章编号:
1008-6455（2018）05-0109-03
作者:
 任润健1; 方 磊1; 贾传龙2; 赵 虎1; 陈 亮2; 秦登科2; 周慧敏1; 毕 波1
Author(s):
 REN Run-jian1; FANG Lei1; JIA Chuan-long2; ZHAO Hu1; CHEN Liang2; QIN Deng-ke2; ZHOU Hui-min1; BI Bo1
关键词:
 ; [关键词]光老化; PDGF-BB; 成纤维细胞; ROS; UVB
分类号:
R758.1
文献标志码:
A
摘要:

[摘要]目的：探讨PDGF-BB对紫外线诱导的光老化成纤维细胞中ROS的影响及抑制氧化应激的作用特点。方法：利用CCK-8法
检查不同浓度PDGF-BB对成纤维细胞增殖活性的影响，筛选治疗最佳浓度；PDGF-BB预处理成纤维细胞48h后，利用UVB体外重
复照射构建成纤维细胞光老化模型；利用β-半乳糖苷酶染色法检测细胞衰老程度；装载活性氧（ROS）检测探针，利用荧光
显微镜、流式细胞技术观察和检测各组ROS的表达。结果：PDGF-BB预处理辐照组（UVB+PDGF-BB组）中β-半乳糖苷酶染色
细胞的着色比率低于UVB组，差异有统计学意义（P ＜0.05）。在UVB诱导的应激性老化中，UVB组ROS表达显著高于正常组（P
＜0.05）；PDGF-BB预处理辐照组（UVB+PDGF-BB组）ROS表达显著低于UVB组（P ＜0.05）。结论：PDGF-BB可降低UVB介导的
光老化成纤维细胞中ROS的表达，减少氧化应激反应，可在一定程度上缓解光老化进程。

Abstract:

Abstract: Objective To explore the effect of PDGF-BB on reactive oxygen species (ROS) in UVB-induced skin fibroblasts
and the characteristics of oxidative stress. Methods The effect of PDGF-BB of different dosages on the activity of fibroblasts
was detected by CCK-8 assay. After pretreatment of fibroblast 48h with PDGF-BB, the photoaging model was successfully
established with UVB in vitro. The senescence degree of cells was detected by the β-galactosidase staining method. ROS test
probe was loaded and oxidative stress of fibroblasts was induced by UVB irradiation in vitro and the expression of ROS was
measured by fluorescence microscopy and flow cell technology respectively. Results The coloring percentage of the cells stained by
beta galactosidase in the PDGF-BB pretreatment group(UVB+PDGF-BB group) was lower than that in the UVB group, the difference
was statistically significant(P＜0.05). In UVB induced stress aging, the expression of ROS in UVB group was significantly higher
than that in normal group(P＜0.05). The expression of ROS in the PDGF-BB pretreatment group (UVB+PDGF-BB group) was
significantly lower than that of UVB group(P＜0.05). Conclusion PDGF-BB can reduce ROS expression in fibroblasts induced by
UVB irradiation and decrease oxidative stress, and it could alleviate photoaging in some extent.
Key words: photoaging; platelet derived growth factor BB(PDGF-BB); fibroblasts; reactive oxygen species(ROS); ultraviolet
radiation B(UVB)

相似文献/References:

[1]. 埋线美容专栏[J].中国美容医学,2018,(01):0.
　[J].Medical Aesthetics and Beauty,2018,(05):0.
[2]杨 震. 应用透明质酸颏部填充联合A型肉毒毒素咬肌注射重塑面部下1/3轮廓的效果观察[J].中国美容医学,2018,(03):1.
　YANG Zhen. Effect of Hyaluronic Acid Chin Filling Combined with Botulinum Toxin Type A Masseter Muscle Injection to Reconstruct the Facial Contour of the Lower 1/3[J].Medical Aesthetics and Beauty,2018,(05):1.
[3]祝闽辉,张栋梁,叶祥柏,等. 皮肤软组织扩张术埋置注液期并发症危险因素研究[J].中国美容医学,2018,(03):4.
　ZHU Min-hui,ZHANG Dong-liang,YE Xiang-bo,et al. Risk Factors of Complications after Tissue Expasion[J].Medical Aesthetics and Beauty,2018,(05):4.
[4]刘申松,杨永成. 自体脂肪颗粒移植联合假体隆乳术在哺乳后乳腺萎缩整形术中的应用[J].中国美容医学,2018,(03):9.
　LIU Shen-song,YANG Yong-cheng. Application of Autologous Fat Granule Graft Combined with Prosthesis Combined Augmentation Mammoplasty in Breast Reconstruction of Breast Atrophy Patients after Breast-feeding[J].Medical Aesthetics and Beauty,2018,(05):9.
[5]韩 星,胡守舵,陈晓芳,等.自体脂肪颗粒移植促进手部年轻化的临床观察[J].中国美容医学,2018,(03):12.
　HAN Xing,HU Shou-duo,CHEN Xiao-fang,et al. The Effect of Autologous Fat Grafting in Hand Rejuvenation[J].Medical Aesthetics and Beauty,2018,(05):12.
[6]李胜旭,苏映军,杨 宽,等. 封闭负压引流技术在先天性并指分指植皮术中的应用[J].中国美容医学,2018,(03):15.
　LI Sheng-xu,SU Ying-jun,YANG Kuan,et al. Application of Vacuum Sealing Drainage for Skin Graft in Patients with Congenital Syndactyly of the Hand[J].Medical Aesthetics and Beauty,2018,(05):15.
[7]陈 波,路 会,马 力,等. 钝针皮下层扇形注射透明质酸矫正面颊部凹陷[J].中国美容医学,2018,(03):18.
　CHEN Bo,LU Hui,MA Li,et al. Correction of Cheek Depression by Fanshaped Hyaluronic Acid Injection in Subcutaneous Layers with Blunt Needle[J].Medical Aesthetics and Beauty,2018,(05):18.
[8]曾德斌,栗鹏程,邱勋永,等. 不同超薄游离穿支皮瓣修复手部软组织缺损效果比较[J].中国美容医学,2018,(03):21.
　ZENG De-bin,LI Peng-cheng,QIU Xun-yong,et al. Comparison on the Repair Effect of Different Ultrathin Free Perforator Flaps in the Treatment of Patients with Soft Tissue Defect of Hand[J].Medical Aesthetics and Beauty,2018,(05):21.
[9]程 定,崔树英,郑 乐,等. 改道引流联合VSD技术修复会阴部撕裂伤的临床研究[J].中国美容医学,2018,(03):24.
　CHENG Ding,CUI Shu-ying,ZHENG Le,et al. Clinical Study on Diverted Drainage Combined with Vacuum Sealing Drainage Technique to Repair the Laceration of the Perineum[J].Medical Aesthetics and Beauty,2018,(05):24.
[10]李云峰,吴泽勇,黄海华,等. 全厚皮片游离移植矫治儿童足背瘢痕挛缩畸形[J].中国美容医学,2018,(03):27.
　LI Yun-feng,WU Ze-yong,HUANG Hai-hua,et al. Full-thickness Skin Graft for the Treatment of Cicatricial Contracture of the Dorsum of Foot in Children[J].Medical Aesthetics and Beauty,2018,(05):27.

更新日期/Last Update: 2018-07-12