[1]张学军,亓发芝. 内质网应激蛋白ATF4调控STAT3信号通路促进黑色素瘤细胞生长及转移的研究[J].中国美容医学,2018,(07):83-86.
 ZHANG Xue-jun,QI Fa-zhi. Regulation of Cell Proliferation and Metastasis by Endoplasmic Reticulum Stress Protein ATF4 via STAT3 Signaling Pathway in Melanoma[J].Medical Aesthetics and Beauty,2018,(07):83-86.
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 内质网应激蛋白ATF4调控STAT3信号通路促进黑色素瘤细胞生长及转移的研究
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2018年07期
页码:
83-86
栏目:
出版日期:
2018-08-10

文章信息/Info

Title:
 Regulation of Cell Proliferation and Metastasis by Endoplasmic Reticulum Stress Protein ATF4 via STAT3 Signaling Pathway in Melanoma
文章编号:
1008-6455(2018)07-0083-04
作者:
 张学军亓发芝
Author(s):
 ZHANG Xue-junQI Fa-zhi
关键词:
 [关键字]黑色素瘤内质网应激ATF4肿瘤细胞增殖肿瘤细胞侵袭STAT3
Keywords:
 Key words: melanoma endoplasmic reticulum stress ATF4 tumor cell proliferation tumor cell invasion STAT3
分类号:
R739.5
文献标志码:
A
摘要:
[摘要]目的:探索内质网应激蛋白ATF4在黑色素瘤发生发展中的作用和机制。方法:运用荧光定量PCR技术分析对比黑色素
瘤细胞系和黑色素细胞系内ATF4表达的差异,运用CCK-8染色以及BrdU染色检测细胞生长及增殖能力,运用Tranwell技术检
测细胞转移能力,运用免疫印迹技术检测细胞内STAT3磷酸化水平。结果:ATF4 mRNA在黑色素瘤细胞中的表达水平显著高于
黑色素细胞,显示肿瘤细胞中含有更高水平的ATF4 mRNA;在A375细胞中过表达ATF4后,细胞生长增殖速率提高,转移能力
增强,而降低内源ATF4后,MM200细胞的增殖速度和转移能力都出现明显降低。同时,A375细胞内ATF4含量的增加也引起了
细胞内STAT3磷酸化水平以及细胞内IL-6表达的显著升高。结论:内质网应激蛋白ATF4在黑色素瘤的发生发展中具有重要的
调控作用,细胞内ATF4可能是参与调控了IL-6-STAT3信号通路,从而促进黑色素瘤细胞增殖和迁移。
Abstract:
Abstract: Objective To explore the role of endoplasmic reticulum (ER) stress protein ATF4 in cell proliferation and metastasis
of melanoma. Methods RT-qPCR was applied to investigate the levels of mRNA expression. CCK-8 staining and BrdU
staining were introduced to determine the abilities of cell growth and proliferation. Transwell system was used to detect the
metastasis ability of cells. Immunoblotting was conducted to determine the protein levels in cells. Results The levels of
ATF4 mRNA significantly increased in melanoma cell lines when compared to melanocytes. Ectopic expression of ATF4
efficiently enhanced the proliferation and metastasis of A375 cells. Abrogation of ATF4 in MM200 impaired cell growth and
metastasis. Furthermore, ATF4 overexpression upregulated the expression of IL-6 and subsequently activated STAT3 signaling.
Conclusion In general, our results revealed that ATF4 regulated cell proliferation and metastasis via activating IL-6-STAT3
signaling in melanoma.

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更新日期/Last Update: 2018-09-07