[1]邱 尧,岳毅刚,邵家松,等. miR-199b促进慢性创面血管化的作用及机制研究[J].中国美容医学,2019,(01):89-92.
 QIU Yao,YUE Yi-gang,SHAO Jia-song,et al. Study on the Role and Mechanism of miR-199b in Promoting Vascularization of Chronic Wound[J].Medical Aesthetics and Beauty,2019,(01):89-92.
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 miR-199b促进慢性创面血管化的作用及机制研究
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2019年01期
页码:
89-92
栏目:
出版日期:
2019-01-15

文章信息/Info

Title:
 Study on the Role and Mechanism of miR-199b in Promoting Vascularization of Chronic Wound
文章编号:
1008-6455(2019)01-0089-04
作者:
 邱 尧岳毅刚邵家松霍 群张 敏
Author(s):
QIU YaoYUE Yi-gangSHAO Jia-songHUO QunZHANG Min
关键词:
 [关键词]微小RNA增殖凋亡分化信号通路慢性创面
Keywords:
 Key words: miRNA proliferation apoptosis differentiation signaling pathwayschronic wound
分类号:
R329.2
文献标志码:
A
摘要:
[摘要]目的:探索miR-199b对HaCaT细胞增殖、迁移及对VEGFA、JAG1、SET信号通路的影响。方法:转染miR-199b上调与下
调慢病毒到人永生化表皮细胞(HaCaT)。分为miR-199b上调(up)、miR-199b下调(dowm)、空载对照(NC组)三个组,
进行CCK8、凋亡、周期试验,并采用RT-PCR技术检测VEGFA、JAG1、SET基因的表达,采用WB技术检测miR-199b对下游蛋白
变化(如VEGFA、JAG1等分子)的影响。结果:miR-199b up组OD450/fold值显著高于NC组,差异有统计学意义(P <0.05)、
miR-199b down组OD450/fold值略低于NC组,差异无统计学意义(P >0.05)。与NC组相比,G1期miR-199b up组与miR-199b
down组的细胞增多(P <0.05);在S期,miR-199b up组及miR-199b down组的细胞减少(P <0.05);在G2/M期,miR-199b
up组及miR-199b down组的细胞减少,差异均具有统计学意义(P <0.05)。与NC组比较,miR-199b up组凋亡细胞百分比下
降(P <0.05)、miR-199b down组凋亡细胞百分比上升(P <0.05);SET在miR-199b up组的表达明显低于NC组,在miR-199b
down组表达明显高于NC组。从定量PCR结果可以看出,HaCaT细胞中,miR-199b up组JAG1表达丰度略高于NC组,差异无统
计学意义(P >0.05),miR-199b down组JAG1表达丰度明显高于NC组(P <0.05);miR-199b up组VEGFA表达丰度明显低于NC组
(P<0.05)、miR-199b down组VEGFA表达丰度明显高于NC组(P<0.05);miR-199b up组SET表达丰度小于NC组(P >0.05)、
miR-199b down组SET表达丰度明显大于NC组(P <0.05)。结论:miR-199b可抑制VEGF、JAG1、SET蛋白的表达,为其靶基因,
可促进细胞的增殖,延长细胞周期,并抑制细胞凋亡。

Abstract:
Abstract: Objective To explore the effects of miR-199b on proliferation and migration of HaCaT cells, as well as VEGFA,
JAG1 and SET signaling pathways. Methods Transfection of miR-199b mimics and inhibitor lentivirus into human
immortalized epidermal cells HaCaT was performed. The study was divided into three groups: miR-199b up, miR-199b down
and no-load control. CCK8, cycle and scratch test were conducted, and the expression of VEGFA, JAG1 and SET genes were
detected by RT-PCR, and the downstream protein changes (such as VEGFA, JAG1 and other molecules) were detected by WB
technology. Results The OD450/fold value of miR-199b up group was significantly higher than that of NC group (P <0.05).
The OD450/fold value of miR-199b down group was slightly lower than that of NC group (P >0.05). In the G1 phase, cells
in the miR-199b up and down group were increased compared to the control group (P <0.05). At the S stage, compared with
the control group, the cells in the miR-199b up and down group were reduced (P >0.05). In the G2/M phase, compared with
the control group, the cells in the miR-199b up and down group were reduced (P >0.05). Compared with the NC group, the
percentage of apoptotic cells in the miR-199b up group decreased (P <0.05) and the percentage of apoptotic cells in the miR-
199b down group increased (P <0.05). The expression of SET was significantly lower in the miR-199b up group than in the NC
group, and significantly higher in the miR-199b down group than in the NC group. According to the quantitative PCR results,
the expression abundance of JAG1 in the miR-199b up group was slightly higher than that in the NC group (P >0.05). The
expression abundance of JAG1 in the miR-199b down group was significantly higher than that in the NC group (P <0.05). The
expression abundance of VEGFA in the miR-199b up group was significantly lower than that in the NC group (P <0.05). The
expression abundance of VEGFA in the miR-199b down group was significantly higher than that in the NC group (P <0.05).
SET expression abundance of miR-199b up group was smaller than that of NC group (P >0.05). The expression abundance of
SET in the miR-199b down group was significantly greater than that in the NC group (P <0.05). Conclusion miR-199b is the
target gene of VEGF, JAG1 and SET proteins by inhibiting their expression.It can promote cell proliferation, prolong cell cycle
and inhibit cell apoptosis.

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更新日期/Last Update: 2019-02-01