虾青素对人源增生性瘢痕成纤维细胞的作用及机制研究<br />-《中国美容医学》

文章信息/Info

Title:: The Study on Effect and Mechanisms of Astaxanthin in Hypertrophic Scar Fibroblast

Keywords:: Key words: astaxanthin; HSF cells; cell proliferation; apoptosis; collagen secretion

摘要:: [摘要]目的:探讨虾青素对人源增生性瘢痕成纤维细胞的作用以及机制研究。方法：体外培养人源增生性瘢痕成纤维细胞分
别加入0、10、50、100μmol/L浓度的虾青素作用24h，MTT法检测细胞增殖抑制率；流式细胞仪检测各组细胞凋亡率；ELISA
法检测细胞中的TIMP-1、MMP-1、COL-I及COL-III的浓度；Western-blotting检测Bcl-2、α-SMA、TGF-β1及Bax等蛋白的表
达。结果: 不同浓度的虾青素使人源增生性瘢痕成纤维细胞活力降低而凋亡率增加；促凋亡蛋白Bax表达上调，抑制凋亡蛋
白Bcl-2表达下调，同时抑制α-SMA、TGF-β1的表达及TIMP-1、MMP-1、COL-I、COL-III合成。结论: 虾青素能诱导人源增生
性瘢痕成纤维细胞增殖、凋亡并抑制HSF细胞增殖、转化，减少胶原分泌，促进胶原酶解，继而有效改善增生性瘢痕的发生
与发展。

Abstract:: Abstract: Objective To explore the effect of astaxanthin on HSF cells and investigate the mechanisms. Methods The
study was divided into two groups, including control group and drug treated group. MTT assay and flow cytometry were
used to determine the cell proliferation inhibition ratio and apoptosis rate of HSF cells treated by astaxanthin with different
concentrations.The expression of TIMP-1, MMP-1,COL-I,COL-III in the culture cell was detected by ELISA. The proteins of
Bcl-2, Bax, were analyzed by Western-blot. Results Astaxanthin can significantly reduce the cell proliferation inhibition ratio
and promote cell apoptosis. Astaxanthin inhibited the TIMP-1,MMP-1,COL-I,COL-III synthesis. Western blot showed that the
expression of pro-apoptotic factor Bax increased, but the expression of anti-apoptotic factor Bcl-2 and α-SMA,TGF-β1 was
suppressed after treatment with astaxanthin. Conclusion Astaxanthin can suppress the HSF proliferation, transformation, and
collagen secretion, and accelerate the collagen and induce apoptosis.