[1]冯 欢,刘仕勇,周 燕,等. miRNA-198靶向调控表皮干细胞内FGFR1表达诱导慢性创面形成的机制研究[J].中国美容医学,2019,(05):76-80.
　FENG Huan,LIU Shi-yong,ZHOU Yan,et al. The Study of miRNA-198 Targeted Regulation of FGFR1 Expression in Epidermal StemCells to Induce the Mechanism of Chronic Wound[J].Medical Aesthetics and Beauty,2019,(05):76-80.
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 miRNA-198靶向调控表皮干细胞内FGFR1表达诱导慢性创面形成的机制研究
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2019年05期
页码:
76-80
栏目:
出版日期:
2019-05-10

文章信息/Info

Title:
 The Study of miRNA-198 Targeted Regulation of FGFR1 Expression in Epidermal Stem
Cells to Induce the Mechanism of Chronic Wound
文章编号:
1008-6455（2019）05-0076-05
作者:
冯 欢1; 刘仕勇2; 周 燕3; 康华利4; 黄小兵3
Author(s):
FENG Huan1; LIU Shi-yong2; ZHOU Yan3; KANG Hua-li4; HUANG Xiao-bing3
关键词:
 ; [关键词]miRNA-198; 慢性创面; 成纤维细胞生长因子受体1; 表皮干细胞; 愈合; 修复
Keywords:
 ; Key words: miRNA-198;  chronic wound;  fibroblast growth factor receptor 1;  epidermal stem cells;  heal;  repair
分类号:
R329.2
文献标志码:
A
摘要:

[摘要]目的：研究miR-198对慢性创面形成的影响，探讨miR-198靶向调控表皮干细胞内成纤维细胞生长因子受体1 (FGFR1)
表达的可能机制与相关性。方法：所有大鼠以表皮干细胞培养划分为三组，对照组20只，采用基础饲料，40只复制糖尿病模
型大鼠组，共32只建糖尿病大鼠慢性创面模型成功，其中16只为慢性创面组，另外16只通过快速尾静脉注射miR-198抑制剂
转染系统溶液，命名为转染抑制载体组，通过免疫组织化学检测表皮干细胞表达，计算慢性创面的愈合率。通过细胞生长曲
线测试和细胞周期分析miR-198对表皮干细胞的影响，实时定量PCR检测各组表皮干细胞中FGFR1表达。用Western-blot法检
测各组细胞中FGFR1及下游Ras和MAPK蛋白的表达。结果：与对照组比较，慢性创面组miR-198表达量明显增高(P ＜0.01)，转
染抑制载体miR-198后，表皮干细胞中表达显著性下调(P ＜0.01)，细胞周期分析发现慢性创面组会抑制表皮干细胞增殖，转
染抑制载体(Anti-miR-198)阻断miR-198后表皮干细胞增殖得到恢复。免疫组化检测显示，慢性创面培养组FGFR1阳性表达数
量增加，随着转染抑制miR-198载体的影响，FGFR1在转染抑制载体组表达增加更明显(P＜0.01)。Western blot检测显示，
在慢性创面模型中，转染抑制载体(Anti-miR-198)后，FGFR1的蛋白表达明显增加，转染抑制载体组Ras和MAPK的蛋白表达明
显降低。结论：miR-198可能负向调控表皮干细胞FGFR1，抑制miR-198可以增强表皮干细胞FGFR1表达，加快表皮干细胞增殖
和分化，从而能促进慢性创面愈合。

Abstract:

Abstract: Objective To investigate the effect of microRNA-198 (miR-198) on chronic wound formation, and the potential
regulation mechanism of miR-198 via targeting fibroblast growth factor receptor 1 (FGFR1) in epidermal stem cells.
Methods Sixty rats were included and divided into three groups according to the cultured epidermal stem cells. Twenty
rats fed with basic diet were set into the control group. The others were in the diabetic model group, and of which, 32 rats
were successfully established as the chronic wounds models. Next, these 32 rats were divided into chronic wound group
(n =16) and transfection inhibitor vector group (n =16, this model were established by rapidly injecting miR-198 inhibitor
transfection system solution via tail vein). In order to calculate the healing rate of chronic wounds, the expression of
epidermal stem cells was detected by immunohistochemistry. In addition, the effect of miR-198 on epidermal stem cells
was further analyzed by cell growth curve test and cell cycle analysis, and the expression of fibroblast growth factor
receptor-1 (FGFR1) in epidermal stem cells was detected by real-time quantitative polymerase chain reaction (PCR) test.
Besides, the Western-blot method was used to detect the FGFR1 expression, and the corresponding downstream Ras and
mitogen-activated protein kinase (MAPK) proteins were also detected. Results By comparing with control group, the
miR-198 expression in the chronic wound group increased significantly (P ＜0.01), and the expression of epidermal stem
cells was significantly decreased in transfection inhibitor vector group (P ＜0.01). In addition, cell cycle analysis showed
that the proliferation of epidermal stem cells was inhibited in chronic wound group, which can be repaired after blocking
miRNA-198 proliferation by transfection inhibition vector (Anti-miR-198). Immunohistochemical results showed that the
FGFR1 expression increased in the chronic wound group, and increased more significantly in the transfection inhibition
vector group (P ＜0.01) underlying the influence of the transfection inhibition vector of miR-198. Western blot analysis
showed that in the transfection inhibition vector group, FGFR1 increased significantly, and Ras and MAPK decreased
significantly. Conclusions miR-198 may negatively regulate epidermal stem cell FGFR1. Inhibition of miR-198 can
enhance the expression of FGFR1 in epidermal stem cells, accelerate the proliferation and differentiation of epidermal
stem cells, and thus promote the healing of chronic wounds.

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更新日期/Last Update: 2019-06-06