[1]李珏丹,崔 敏,许 莹,等. 氟对体外培养LS8细胞增殖、氧化应激与凋亡的作用研究[J].中国美容医学,2019,(09):89-92.
　LI Jue-dan,CUI Min,XU Ying,et al. Effect of Fluoride on Cell Proliferation, Oxidative Stress and Apoptosis in LS8 Cells[J].Medical Aesthetics and Beauty,2019,(09):89-92.
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 氟对体外培养LS8细胞增殖、氧化应激与凋亡的作用研究
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2019年09期
页码:
89-92
栏目:
出版日期:
2019-09-05

文章信息/Info

Title:
 Effect of Fluoride on Cell Proliferation, Oxidative Stress and Apoptosis in LS8 Cells
文章编号:
1008-6455（2019）09-0089-04
作者:
李珏丹1; 2; 崔 敏1; 2; 许 莹1; 2; 郭 晶1; 2; 阮建平1; 3; 程 政1; 2
Author(s):
LI Jue-dan1; 2; CUI Min1; 2; XU Ying1; 2; GUO Jing1; 2; RUAN Jian-ping1; 3; CHENG Zheng1; 2
关键词:
 ; [关键词]氟化物; LS8细胞; 增殖; 氧化应激; 凋亡
Keywords:
 ; Key words: fluoride;  LS8 cell;  proliferation;  oxidative stress;  apoptosis
分类号:
R329.2+8
文献标志码:
A
摘要:
[摘要]目的：观察氟化物对小鼠成釉细胞样细胞（LS8细胞）增殖、氧化应激以及凋亡的影响。方法：取对数生长期的LS8
细胞，在培养液中分别加入终浓度为0、0.5、1、2、4和8mM的氟化钠（NaF）溶液，培养24h和48h后，CCK-8检测细胞增
殖；倒置显微镜观察细胞形态；生化方法检测细胞丙二醛（MDA）含量及超氧化物歧化酶（SOD）活性；qRT-PCR检测过氧化
氢酶（Cat）、谷胱甘肽过氧化物酶1（Gpx-1）和谷胱甘肽S转移酶（Gst）mRNA水平；流式细胞术检测细胞凋亡；Western
blotting检测caspase-9和caspase-3蛋白表达改变。结果：过量氟抑制LS8细胞增殖，且随着NaF浓度增加，细胞增殖呈下降
趋势；过量氟显著增加LS8细胞内MDA含量，降低SOD活性及Gpx-1、Gst mRNA表达水平；过量氟引起LS8细胞形态改变和细胞
凋亡，且伴随着caspase-9，caspase-3表达的显著增加。结论：过量氟抑制LS8细胞增殖，扰乱细胞的氧化与抗氧化平衡并
诱发氧化应激，持续的氧化应激最终导致细胞凋亡。

Abstract:
Abstract: Objective To investigate the effects of fluoride on cell proliferation, oxidative stress and apoptosis in ameloblast-like
cells (LS8 cells). Methods LS8 cells were treated with NaF at 0, 0.5, 1, 2, 4, 8mM for 24 and 48h, respectively. The effect of
fluoride on cell viability, apoptosis and cell changes were assayed by CCK-8, flow cytometry and microscope, separately. Some
well-known regulators of oxidative stress and mitochondrial pathway of apoptosis were assessed by biochemical assay, qRTPCR
and western blotting. Results The results showed significantly decreased of cell viability and increased cell apoptosis in
NaF-treated LS8 cells. Besides, NaF treatment in LS8 cells markedly decreased MDA content, while increased SOD activity,
the mRNA level of Gpx-1 and Gst, and the protein levels of cleaved caspase-9 and cleaved caspase-3. Conclusion Fluoride
exposure could suppress cell proliferation, disorder the balance between oxidative system and anti-oxidant system and cause
oxidative stress, and finally result in apoptosis of LS8 cells.

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更新日期/Last Update: 2019-09-27