萝卜硫素通过lncRNA MALAT1/TFEB信号通路诱导人皮肤鳞状细胞癌细<br />胞凋亡机制探讨<br />-《中国美容医学》

文章信息/Info

Title:: Mechanism of Apoptosis of Human Skin Squamous Cell Carcinoma Cells Induced
by Sulforaphane Through LncRNA MALAT1/TFEB Pathway

Keywords:: Key words: sulforaphane; human skin squamous cell carcinoma; apoptosis; proliferation; lncRNA MALAT1/TFEB signalingpathway; mechanism

摘要:: [摘要]目的：观察萝卜硫素对人皮肤鳞状细胞癌A431细胞凋亡和lncRNA MALAT1/TFEB信号通路的影响。方法：CCK-8法和流
式细胞术分析萝卜硫素对A431细胞活力、增殖及凋亡相关指标的影响；倒置显微镜观察A431细胞的克隆数；RT-PCR法分析
lncRNA MALAT1 mRNA表达水平的影响；Western blot方法分析萝卜硫素对A431细胞中凋亡相关蛋白、TFEB及TFEB(Ser142)磷
酸化水平的影响。结果：与0μg/ml萝卜硫素组比较，浓度大于10μg/ml萝卜硫素组细胞活力、克隆细胞数、BrdU偶联细胞
数、S和G2期细胞数、lncRNA MALAT1 mRNA表达水平均明显降低（P ＜0.05）；而G1期细胞数、Caspase-3/9活性、组蛋白DNA
水平、细胞凋亡率及TFEB表达水平显著增高（P ＜0.05）；A431细胞中过表达MALAT1，能明显降低萝卜硫素对TFEB(Ser142)磷酸
化水平和细胞活力的抑制作用（P ＜0.05）。结论：萝卜硫素可通过诱导A431细胞凋亡和抑制细胞增殖发挥抗肿瘤作用，其
机制可能与抑制lncRNA MALAT1/TFEB信号通路活化有关。

Abstract:: Abstract: Objective To observe the effects of sulforaphane on the apoptosis of skin squamous cell carcinoma A431 cells and
lncRNA MALAT1/TFEB pathway. Methods The effects of sulforaphane on the cell viability, proliferation and apoptosis
were respectively measured by using CCK-8 assay and flow cytometry assay. Inverted microscope was used to analyze
cell colonies. RT-PCR was used to determine the expression level of lncRNA MALAT1 mRNA. Western blot was used to
investigate the impacts of sulforaphane on the expression levels of apoptotic proteins, TFEB and TFEB(Ser142) in A431 cells.
Results Compared with 0μg/ml of sulforaphane group, the cell viability, cell colonies, BrdU- coupling cells, cells in S and G2
phase, the expression level of lncRNA MALAT1 mRNA were significantly decreased in more than 10μg/ml of sulforaphane
groups (P ＜0.05). In addition, cells in G1 phase, the activities of Caspase-3/9, histone DNA level, cell apoptotic rates and the
expression level of TFEB were significantly increased (P ＜0.05). The overexpression of MALAT1 in A431 cells could decrease
the inhibitory effects of sulforaphane on the TFEB(Ser142) phosphorylation level and cell viability (P ＜0.05). Conclusion
Sulforaphane may play an anti-tumor effect by inducing human skin squamous cell carcinoma apoptosis and suppressing their
proliferation, and its mechanism may be associated with the decreased activation of lncRNA MALAT1/TFEB pathway.

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