miR-506-3p靶向Beclin-1调控烧伤后皮肤成纤维细胞的自噬和增殖<br />机制研究<br />-《中国美容医学》

文章信息/Info

Title:: miR-506-3p Targets Beclin-1 to Regulate Autophagy and Proliferation of Post-burn
Skin Fibroblasts

Author(s):: SHI Min1; DING Xin-qiang2; GUO Xiao-bo3; CHEN Wei-ying1; ZHANG Ming-yu1

Keywords:: Key words: miR-506-3p; Beclin-1; fibroblasts; autophagy; proliferation; burn

摘要:: [摘要]目的：探讨烧伤后皮肤成纤维细胞中miR-506-3p表达与Beclin-1的靶向关系及增殖调控作用，为烧伤后瘢痕的治疗
提供新靶点。方法：采用实时定量聚合酶链反应（qRT-PCR）检测烧伤组织和热损伤皮肤成纤维细胞中miR-506-3p水平；
Targetscan在线预测，双荧光素酶验证miR-506-3p与Beclin-1靶向关系；qRT-PCR和Western blot检测miR-506-3p模拟物或
抑制剂转染对Beclin-1表达的影响；qRT-PCR检测热刺激后miR-506-3p模拟物（10nM或30nM）转染成纤维细胞miR-506-3p的
表达并采用CCK-8检测转染细胞增殖效率。结果：与正常组织相比，烧伤组织和热损伤成纤维细胞中miR-506-3p的表达下
调；miR-506-3p和Beclin-1存在靶向调控作用关系，并且miR-506-3p可负调控Beclin-1表达；miR-506-3p模拟物转染以剂量
依赖的方式抑制了热刺激的真皮成纤维细胞中的细胞增殖。结论：miR-506-3p通过靶向抑制Beclin-1表达来调节烧伤后皮肤
成纤维细胞的自噬和增殖，有可能成为烧伤愈合后瘢痕治疗的潜在靶标。

Abstract:: Abstract: Objective To explore the targeting relationship between the expression of miR-506-3p and Beclin-1 in heat-damaged
skin fibroblasts and its proliferation regulation effect on Beclin-1, in order to provide a new target for the treatment of post-burn
scars. Methods We used qRT-PCR to detect miR-506-3p of post-burn tissue and heat-damaged skin fibroblasts, used Targetscan
online prediction and dual luciferase to verify the targeting relationship between miR-506-3p and Beclin-1. We used qRT-PCR
and Western blot to detect the effect of miR-506-3p mimic or inhibitor transfection on Beclin-1 expression. The expression of
miR-506-3p in fibroblasts transfected with miR-506-3p mimic (10nM or 30nM) after thermal stimulation was detected by qRTPCR
and the proliferation efficiency of transfected cells was detected by CCK-8. Results Compared with normal tissues, the
expression of miR-506-3p in post-burn tissues and heat-damaged fibroblasts is down-regulated. There is a targeted regulation
relationship between miR-506-3p and Beclin-1, and miR-506-3p can negatively regulate the expression of Beclin-1, and
miR-506-3p mimic transfection inhibits cell proliferation in heat-stimulated dermal fibroblasts in a dose-dependent manner.
Conclusion MiR-506-3p regulates the autophagy and proliferation of post-burn skin fibroblasts by targeting the expression of
Beclin-1, and may be a potential target on scar for post-burn healing.