[1]陈国勇,吴 芳.miRNA-132通过Wnt/β-catenin信号通路促进牙周膜干细胞成骨分化的研究[J].中国美容医学,2022,(6):88-92.
　CHEN Guoyong,WU Fang.miRNA-132 Promotes Osteogenic Differentiation of Periodontal Ligament Stem Cells through Wnt/-catenin Signaling Pathway[J].Medical Aesthetics and Beauty,2022,(6):88-92.
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miRNA-132通过Wnt/β-catenin信号通路促进牙周膜干细胞成骨分化的研究()
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2022年6期
页码:
88-92
栏目:
出版日期:
2022-06-10

文章信息/Info

Title:
miRNA-132 Promotes Osteogenic Differentiation of Periodontal Ligament Stem Cells through Wnt/-catenin Signaling Pathway
文章编号:
1008-6455（2022）06-0088-05
作者:
陈国勇; 吴 芳
(株洲市中心医院口腔科 湖南 株洲 412007 )
Author(s):
CHEN Guoyong; WU Fang
(Department of Stomatology,Zhuzhou Central Hospital,Zhuzhou 412007,Hunan,China)
关键词:
miRNA-132; Wnt/β-catenin; 牙周膜干细胞; 成骨分化; ALP; Runx2; OCN
Keywords:
miRNA-132;  Wnt/-Catenin;  PDLSCs;  osteogenic diff erentiation;  ALP;  Runx2;  OCN
分类号:
R780.2
文献标志码:
A
摘要:
目的：探讨miRNA-132通过Wnt/β-catenin信号通路促进牙周膜干细胞的成骨分化。方法：细胞培养，观察牙周膜干细胞（periodontal ligament stem cells，PDLSCs）形态。成骨诱导，检测成骨样分化情况。成脂诱导，检测成脂分化情况。将PDLSCs细胞分为3组，分别为对照组、miRNA-132模拟组和miRNA-132抑制剂组，采用八肽胆囊收缩素（Cholecystokinin octapeptide，CCK-8）检测PDLSCs细胞增殖，酶联免疫检测碱性磷酸酶（Alkaline phosphatase protein，ALP）活性，茜素红染色检测矿化结节沉积，采用实时荧光定量PCR（Real-time quantitative PCR，qRT-PCR）法检测成骨相关基因，采用Western blot检测Wnt/β-catenin相关通路蛋白。结果：miRNA-132模拟组的PDLSCs细胞增殖能力、ALP活性及矿化结节数量显著高于对照组，miRNA-132抑制剂组明显低于对照组和miRNA-132模拟组，差异有统计学意义（P＜0.05)。miRNA-132模拟组中的ALP、Runx2、OCN和Osterix mRNA的相对表达明显高于对照组，miRNA-132抑制剂组显著低于对照组和miRNA-132模拟组，差异均有统计学意义（P＜0.05）。miRNA-132模拟组中的GSK3β和β-catenin蛋白的表达明显高于对照组，miRNA-132抑制剂组明显低于对照组和miRNA-132模拟组，差异有统计学意义（P＜0.05）。XAV939组中的ALP、Runx2、OCN和Osterix mRNA的相对表达明显低于对照组，miRNA-132模拟组表达高于对照组和XAV-939组，miRNA-132模拟组+XAV-939组表达高于XAV-939组，差异有统计学意义（P＜0.05）。结论：过表达miRNA-132可以促进牙周膜干细胞的成骨分化，抑制miRNA-132表达从而抑制了人牙周膜干细胞的成骨分化，miRNA-132调节牙周膜干细胞的成骨分化，其机制可能是通过Wnt/β-catenin信号通路来实现的。
Abstract:
Objective To investigate the eff ect of miRNA-132 on osteogenic diff erentiation of periodontal ligament stem cells through Wnt / β - catenin signaling pathway. Methods The morphology of PDLSCs was observed by cell culture. Osteogenic induction and osteogenic differentiation were detected. Adipogenic induction and adipogenic differentiation were detected. PDLSCs cells were divided into three groups: the control group, the miRNA-132 simulation group and the miRNA-132 inhibitor group. The proliferation of PDLSCs cells was detected by CCK-8. ALP activity was detected by enzyme-linked immunosorbent assay. Mineralized nodule deposition was detected by alizarin red staining. Osteogenesis related genes were detected by qRTPCR, and Wnt/β-catenin related pathway protein was detected by Western blot. Results TThe proliferation ability, ALP activity and the number of mineralized nodules of PDLSCs in the miRNA-132 simulation group were signifi cantly higher than those in the control group, the diff erences were statistically signifi cant (P＜0.05). The miRNA-132 inhibitor group was signifi cantly lower than the control group and miRNA-132 simulation group (P＜0.05). The relative expression of ALP, Runx2, OCN and Osterix mRNA in the miRNA-132-132 simulation group was significantly higher than those in the control group, and the miRNA-132 inhibitor group was signifi cantly lower than the control group and miRNA-132 simulation group (P＜0.05). The expression of GSK3β and β-catenin protein in the miRNA-132 simulation group was signifi cantly higher those that in control group, and the miRNA-132 inhibitor group was signifi cantly lower than the control group and miRNA-132 simulation group (P＜0.05). The relative expression of ALP, Runx2, OCN and Osterix mRNA in the XAV-939 group was signifi cantly lower than those in the control group (P＜0.05). The expression in the miRNA-132 simulation group was higher than those in the control group and the XAV-939 group (P＜0.05). The expression in the miRNA-132 simulation + XAV-939 group was higher than those in the XAV-939 group (P＜0.05). Conclusion Overexpression of miRNA-132 can promote the osteogenic diff erentiation of periodontal ligament stem cells, and inhibit the expression of miRNA-132, thus inhibiting the osteogenic diff erentiation of human periodontal ligament stem cells. The mechanism of miRNA-132 regulating the osteogenic diff erentiation of periodontal ligament stem cells may be through Wnt/β-catenin signaling pathway
更新日期/Last Update: 2022-07-04