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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:

期数:

2023年6期

页码:

112-117

栏目:

出版日期:

2023-06-10

文章信息/Info

Title:

Preparation of Quercetin Nano Silver Antibacterial Gauze and its Antibacterial Experiment in Vitro?/html>

文章编号:

1008-6455（2023）06-0112-06

作者:

许林林; 曹斐然; 吴晓琴; 邱 华; 王银龙

(安徽医科大学口腔医学院/附属口腔医院·口腔疾病研究安徽省重点实验室 安徽 合肥 230032 )

Author(s):

XU Linlin; CAO Feiran; WU Xiaoqin; QIU Hua; WANG Yinlong

(Department of Stomatology/Affi liated Stomatological Hospital of Anhui Medical University,Anhui Provincial Key Laboratory of Oral Disease Research,Hefei 230032,Anhui,China)

关键词:

槲皮素; 纳米银; 原位还原; 绿色制备; 抗菌活性; 复合抗菌纱布

Keywords:

quercetin;  nano silver;  in-situ reduction;  green preparation;  antimicrobial activity;  composite antibacterial gauze

分类号:

R644

文献标志码:

A

摘要:

目的：在纱布表面通过原位还原法制备槲皮素-纳米银复合抗菌涂层，并探究其抗菌效率及生物相容性。方法：利用 槲皮素易溶于碱难溶于酸的特性，通过碱酸重析出的方法在纱布表面形成均匀的非晶包裹层，将其浸入硝酸银溶液中，制 备出槲皮素-纳米银复合抗菌纱布。扫描电镜（Scanning electron microscope，SEM）观察样品表面形貌、X射线光电子能 谱(X-ray photoelectron spectroscopy，XPS)分析表面元素组成、能量散布分析仪(Energy Dispersive Spectrometer， EDS) 分析样品表面元素分布情况，琼脂平面扩散法试验、吸收法实验评价抗菌性能；通过CCK-8法评价(Cell Counting Kit8)细胞毒性；利用结晶紫染色法在显微镜下观察细胞的数目形态。结果：SEM显示槲皮素-纳米银复合抗菌纱布组（Cotton gauze- quercetin/Ag group，CG-Q/Ag组）纱布表面形成光滑槲皮素包裹层，在槲皮素包裹层表面通过原位还原反应成功搭 载了纳米银颗粒，EDS检测显示CG-Q/Ag组纳米银成功搭载在纱布纤维表面并且均匀分布；抗菌结果显示琼脂平面扩散法试验 中CG-Q/Ag0.5、CG-Q/Ag0.1组周围有透亮的抑菌环，吸收法实验中CG-Q/Ag0.5、CG-Q/Ag0.1抑菌率＞95%优于其余各组，且差异具 有统计学意义（P＜0.05）；CCK-8实验中显示CG-Q/Ag0.5具有良好的生物相容性；结论：槲皮素-纳米银复合抗菌纱布在实现 有效抑菌能力时还具备良好的生物相容性，碱酸重析出法构建槲皮素非晶包裹层并且原位还原生成银纳米颗粒的策略为纱布 载银改性提供了一种新的技术路线。

Abstract:

Objective Quercetin nano silver composite antibacterial coating was prepared on the surface of gauze by in-situ reduction method. At the same time, its antibacterial effi ciency and biocompatibility were explored. Methods Taking advantage of the characteristics that quercetin is easy to dissolve in alkali but diffi cult to dissolve in acid, a uniform amorphous coating is formed on the surface of the gauze by alkali acid precipitation method. Then it was immersed in silver nitrate solution to prepare quercetin nano silver composite antibacterial gauze.The surface morphology of the samples was observed by scanning electron microscope (SEM);the surface element composition was analyzed by X-ray photoelectron spectroscopy (XPS);the antibacterial properties were evaluated by agar plane diff usion test and absorption test. Cytotoxicity was evaluated by CCK-8 method; Crystal violet staining was used to observe the number and morphology of cells under the microscope. Results SEM showed that smooth quercetin coating layer was formed on the surface of CG-Q/Ag group gauze, and nano silver particles were successfully loaded on the surface of quercetin coating layer through in-situ reduction reaction. EDS showed that the CG-Q/ Ag group nano-silver was successfully and uniformly loaded on the surface of the gauze fi ber. In agar plane diff usion test, the antibacterial results showed that there were clear antibacterial rings around CG-Q/Ag0.5 and CG-Q/Ag0.1; the antibacterial rates of CG-Q/Ag0.5 and CG-Q/Ag0.1 in absorption test were better than those of other groups, and the diff erence was statistically signifi cant (P ＜0.05);CCK-8 experiment showed that CG-Q/Ag0.5 had good biocompatibility. Conclusion Quercetin-nanosilver composite antibacterial gauze has good biocompatibility while achieving effective bacteriostatic ability. Strategy, that use the alkaline acid reprecipitation method to construct the quercetin amorphous coating and in situ reduction to generate silver nanoparticles, provides a new green technical route for the modifi cation of gauze carrying silver.

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更新日期/Last Update: 2023-07-11