[1]黄 芳,周利丹,卢伊娜.紫苜蓿提取物抵抗光损伤的作用及其机理研究[J].中国美容医学,2024,(1):58-62.[doi:10.15909/j.cnki.cn61-1347/r.006107]
　HUANG Fang,ZHOU Lidan,LU Yina.Mechanism of the Medicago Sativa Extract Against Ultraviolet and Blue Light Induced Photodamage[J].Medical Aesthetics and Beauty,2024,(1):58-62.[doi:10.15909/j.cnki.cn61-1347/r.006107]
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紫苜蓿提取物抵抗光损伤的作用及其机理研究()
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2024年1期
页码:
58-62
栏目:
出版日期:
2024-01-10

文章信息/Info

Title:
Mechanism of the Medicago Sativa Extract Against Ultraviolet and Blue Light Induced Photodamage
文章编号:
1008-6455（2024）01-0058-05
作者:
黄 芳; 周利丹; 卢伊娜
(上海珈凯生物股份有限公司 上海 200241 )
Author(s):
HUANG Fang; ZHOU Lidan; LU Yina
(JAKA BIOTECH Co.,LTD.,Shanghai 200241,China)
关键词:
紫苜蓿提取物; 光损伤; 抗氧化; 高频蓝光; 紫外线; 抗皱
Keywords:
Medicago Sativa extract;  photodamage;  antioxidant;  high-energy visible light;  ultraviolet;  anti-wrinkle
分类号:
R285
DOI:
10.15909/j.cnki.cn61-1347/r.006107
文献标志码:
A
摘要:
目的：通过多种评价方法，探究紫苜蓿提取物（Medicago Sativa extract,MS）抵抗光损伤的作用及其机理。方 法：通过体外清除ABTS自由基模型，高频蓝光（High-energy visible light,HEVL）照射角质形成细胞（HaCaT）产生 ROS 自由基模型，紫外线照射HaCaT后细胞活力、产生前列腺素E2（Prostaglandin E2,PGE2）和基质金属蛋白酶1（Matrix metalloproteinase 1,MMP-1）模型及HEVL诱导的人体皮肤损伤模型来验证MS的抗光损伤功效。结果：MS具有良好的抗氧化 能力，其清除ABTS自由基的IC50为0.048%，质量分数为0.25%～0.5%的MS可显著减少HEVL照射下的HaCaT细胞中ROS活性氧的 产生，质量分数为0.1%～0.3%的MS剂量依赖性地增加紫外线辐照损伤模型中细胞存活率，并降低PGE2和MMP-1的表达量。在 HEVL 诱导的人体皮肤损伤模型中，质量分数为0.5%～5%的MS能明显降低皮肤红斑的形成，增加皮肤亮度，减少黑色素的生 成。结论：MS作为一种植物来源的抗光损伤型原料，可应用于舒缓、修护及抗皱类功效宣称的产品中。
Abstract:
Objective To explore the effect and mechanism of Medicago Sativa extract (MS) against light damage through various evaluation methods. Methods The ABTS free radical detection model was built in vitro, the ROS free radical detection model was produced by high energy visible light (HEVL) irradiation on keratinocytes (HaCaT), the cell viability, production of prostaglandin E2 (PGE2) and matrix metalloproteinase-1 (MMP-1) models after UV irradiation on HaCaT, and the human skin injury model induced by HEVL were used to verify the effi cacy of MS. Results MS had a good antioxidant capacity. The IC50 of MS scavenging ABTS free radicals was 0.048%. MS with a mass fraction of 0.25%-0.5% could signifi cantly reduce ROS production in HaCaT cells exposed to HEVL. MS with a mass fraction of 0.1%-0.3% increased the cell survival rate in UV irradiation injury model in a dose-dependent manner, and decreased the expression of prostaglandin E2 (PGE2) and matrix metalloproteinase-1 (MMP-1). In the human skin injury model induced by HEVL, MS with a mass fraction of 0.5%-5% can signifi cantly reduce the formation of skin erythema, increase skin brightness and reduce the formation of melanin. Conclusion As a kind of anti photodamage raw material from plants, MS can be used in the products claimed to have soothing, repairing and anti wrinkle eff ects.

相似文献/References:

[1]倪 娜,周炳荣,马委委,等. miRNA-769-5p上调对正常人成纤维细胞生物活性的影响[J].中国美容医学,2018,(04):109.
　NI Na,ZHOU Bing-rong,MA Wei-wei,et al. Effect of miRNA-769-5p on Biological Activity of Normal Human Fibroblasts[J].Medical Aesthetics and Beauty,2018,(1):109.
[2]焦丹丹 唐亚楠 高 腾 综述,任贵云 张 蒙 审校. 多酚类化合物在皮肤光损伤防护方面的研究进展[J].中国美容医学,2019,(08):158.
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更新日期/Last Update: 2024-01-24