[1]陈福扬,孙艳艳,谭金娣,等. 牛磺熊去氧胆酸诱导牙髓干细胞的成骨分化实验研究[J].中国美容医学,2018,(04):97-101.
　CHEN Fu-yang,SUN Yan-yan,TAN Jin-di,et al. Taursodeoxycholic Acid Induced the Osteogentic Differentiation Ability of Dental Pulp Stem Cells[J].Medical Aesthetics and Beauty,2018,(04):97-101.
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 牛磺熊去氧胆酸诱导牙髓干细胞的成骨分化实验研究
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2018年04期
页码:
97-101
栏目:
出版日期:
2018-05-10

文章信息/Info

Title:
 Taursodeoxycholic Acid Induced the Osteogentic Differentiation Ability of Dental Pulp Stem Cells
文章编号:
1008-6455（2018）03-0097-04
作者:
 陈福扬1; 孙艳艳2; 谭金娣1; 于易秀1; 刘明月1; 史 欣1; 胡伟平1
Author(s):
 CHEN Fu-yang1; SUN Yan-yan2; TAN Jin-di1; YU Yi-xiu1; LIU Ming-yue1; SHI Xin1; HU Wei-ping1
关键词:
 ; [关键词]牙髓干细胞; 牛磺熊去氧胆酸; 细胞增殖; 成骨分化; 新型生物材料; 组织工程
Keywords:
 ; Key words: dental pulp stem cells;  taursodeoxycholic acid;  cell proliferation;  osteogenic differentiation;  new biomaterials; tissue engineering
分类号:
R329.2
文献标志码:
A
摘要:

[摘要]目的：研究牛磺熊去氧胆酸（Tauroursodeoxycholic acid，TUDCA）对牙髓干细胞 (Dental pulp stem cells，
DPSCs)增殖及成骨向分化的作用。方法：用不同质量浓度(0、10、100、30OnM)的TUDCA培养DPSCs，利用甲基噻唑基四唑
（Methylthiazolylterrazolium，MTT)法计数检测不同浓度TUDCA对细胞增殖的影响；7d后测定不同浓度TUDCA诱导后的碱性
磷酸酶(Alkaline phosphatase，ALP)活性；倒置相差显微镜下观察诱导后的DPSCs形态变化；应用茜素红染色法检测矿化结
节的形成。结果：MTT法及ALP活性测定实验表明，随着TUDCA浓度的增加，ALP活性增强，细胞增殖率升高。茜素红染色显示
TUDCA诱导培养28d后出现矿化结节。结论：TUDCA可促进DPSC增殖和成骨分化。

Abstract:
Abstract: Objective To study the effect of tauroursodeoxycholic acid (TUDCA) on the proliferation and osteogenic
differentiation in dental pulp stem cells (DPSCs). Methods Methylthiazolyltetrazolium (MTT) assay was used to evaluate
the proliferation rate of DPSCs induced by different concentrations of TUDCA(0,10,100,300nM). The activity of alkaline
phosphatase (ALP) after induced by different concentration of TUDCA was measured. We observed the morphologica1 changes
of DPSCs by the inverted phase contrast microscope, detected the mineralize nodules stained by Alizarin red. Results The
results of MTT and ALP activity measurement showed that ALP activity increased and cell proliferation rate arise with the
increase of concentration of TUDCA. Alizarin red staining showed that mineralized nodules have formed after induced by
TUDCA for 28 day. Conclusion TUDCA can promote the proliferation and osteogenic differentiation of DPSCs.

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更新日期/Last Update: 2018-06-19