[1]陈向齐,宋洪涛,陈胜平,等. 5-氨基酮戊酸光动力对大鼠痤疮模型免疫功能的影响[J].中国美容医学,2018,(06):51-54.
　CHEN Xiang-qi,SONG Hong-tao,CHEN Sheng-ping,et al. Effect of 5-ALA-PDT on the Toll-like Receptor-mediated Immune Response in Acne Model in SD Rats[J].Medical Aesthetics and Beauty,2018,(06):51-54.
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 5-氨基酮戊酸光动力对大鼠痤疮模型免疫功能的影响
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2018年06期
页码:
51-54
栏目:
出版日期:
2018-07-10

文章信息/Info

Title:
 Effect of 5-ALA-PDT on the Toll-like Receptor-mediated Immune Response in Acne Model in SD Rats
文章编号:
1008-6455（2018）06-0051-04
作者:
 陈向齐1; 宋洪涛2; 陈胜平1; 林 兵2; 刘志宏2; 桑军军1; 昊 霞2
Author(s):
 CHEN Xiang-qi1; SONG Hong-tao2; CHEN Sheng-ping1; LIN Bing2; LIU Zhi-hong2; SANG Jun-jun1; HAO Xia2
关键词:
 ; [关键词]5-氨基酮戊酸; 光动力; 痤疮丙酸杆菌; 痤疮; CD68; CD163; TLR2; MyD88
Keywords:
 ; Key words: 5-aminolevulinic acid;  photodynamic therapy;  Propionibacterium acnes;  acne vulgaris;  CD68;  CD163;  TLR2;  MyD88
分类号:
R758.73+3
文献标志码:
A
摘要:
[摘要]目的：探讨5-氨基酮戊酸光动力（5-aminolevulinicacid photodynamic therapy，5-ALA-PDT）对大鼠痤疮模型局部免疫
功能的影响及相关机制。方法：将痤疮丙酸杆菌接种大鼠耳部，建立大鼠痤疮炎症模型，给予5-ALA-PDT治疗。对组织进行HE染
色，采用免疫组化法检测大鼠巨噬细胞CD68、CD163、Toll样受体2（Toll-like receptor-2，TLR2）、髓样分化因子88（MyD88）
的表达。结果：痤疮丙酸杆菌接种于大鼠耳部可建立大鼠痤疮炎症模型，HE染色见大量炎性细胞弥漫性浸润。CD68、CD163、
TLR2、MyD88在大鼠耳部皮肤炎症细胞质内表达，空白组较少，接种P.acnes后表达增高，加激动剂Pam3CSK4后表达更高，5-ALAPDT
治疗后较P.acnes组低。结论：大鼠耳部毛囊皮脂腺及周围可少量表达TLR2，接种痤疮丙酸杆菌后，表达显著增加，导致巨噬
细胞等免疫细胞向毛囊皮脂腺周围聚集。5-ALA-PDT治疗后TLR2表达减少，减弱机体对细菌的免疫反应，从而减轻炎症。
Abstract:
Abstract: Objective To study the influence of 5-ALA-PDT on the Toll-like receptor-mediated immune response in acne
model in SD rats. Methods Acne inflammation model of rat ear induced by Propionibacterium acnes were established and
then treated with 5-ALA-PDT. Section was made for HE staining. Immunohistochemical staining was used to observe the
expression of CD68, CD163, TLR2, and MyD88. Results Acne inflammation model of rat ear was established by inoculating
Propionibacterium acnes to rat ears. Diffuse infiltrations of inflammatory cell were observed by HE staining. Compared with
the blank group, the expression of CD68, CD163, TLR2, MyD88 were decreased in rat ears inflammatory cells, but increased
after stimulated with P.acnes and increased further after Pam3CSK4 adminstration. The expression level decreased and was
lower than the model group after received 5-ALA-PDT. Conclusion TLR2 expression was low in rat ear pilosebaceous unit
and its surrounding tissue and the expression increased significantly after inoculating Propionibacterium acnes, which induced
macrophage infitration in pilosebaceous unit. 5-ALA-PDT decreased the expression of TLR2 and alleviates body’s immune
response to bacteria, thus relieved inflammation

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更新日期/Last Update: 2018-08-08