[1]朱明宇,李雨珊,王志国,等. 机械牵张力加载时间对诱导正常皮肤成纤维细胞向增生性瘢痕成纤维细胞转化过程的影响研究[J].中国美容医学,2018,(06):55-59.
 ZHU Ming-yu,LI Yu-shan,WANG Zhi-guo,et al. The Study of the Effect of Mechanical Stretch Loading Time on the Transformation of Normal Skin Fibroblasts to Hypertrophic Scar Fibroblasts[J].Medical Aesthetics and Beauty,2018,(06):55-59.
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 机械牵张力加载时间对诱导正常皮肤成纤维细胞向增生性瘢痕成纤维细胞转化过程的影响研究
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2018年06期
页码:
55-59
栏目:
出版日期:
2018-07-10

文章信息/Info

Title:
 The Study of the Effect of Mechanical Stretch Loading Time on the Transformation of Normal Skin Fibroblasts to Hypertrophic Scar Fibroblasts
文章编号:
1008-6455(2018)06-0055-05
作者:
 朱明宇1李雨珊2王志国3匡瑞霞3
Author(s):
 ZHU Ming-yu1LI Yu-shan2WANG Zhi-guo3KUANG Rui-xia3
关键词:
[关键词]增生性瘢痕机械张力成纤维细胞细胞转化
Keywords:
Key words: hypertrophic scar mechanical stress fibroblasts cell transformation
分类号:
R619+.6
文献标志码:
A
摘要:
[摘要]目的:探究机械牵张力不同加载时间对诱导正常皮肤成纤维细胞(Normal skin fibroblasts, NSFB)向增生性瘢痕
成纤维细胞(Hypertrophic scar fibroblasts, HSFB)转化的影响。方法:同一人体NSFB和HSFB分别设实验组和对照组,
实验组加载0.1Hz、10%拉伸幅度的机械牵张力3d、5d、7d,对照组细胞均不加力。CCK-8法检测细胞增殖能力;SYBR Green
qPCR法检测细胞p130Cas、Integrin-β1、转化生长因子-β1(TGF-β1)、Ⅰ型胶原、α-平滑肌肌动蛋白(α-SMA)mRNA表
达水平;ELISA法检测细胞上清液TGF-β1和Ⅰ型胶原含量;Western-blot法检测细胞Integrin-β1、p130Cas、α-SMA蛋白表
达水平。结果:机械牵张力加载5d时,NSFB实验组与HSFB对照组比较,细胞增殖水平及p130Cas、Integrin-β1、TGF-β1、
Ⅰ型胶原、α-SMA表达水平相近,差异均无统计学意义(P >0.05);加载3d、7d时,以上指标检测结果差异显著,差异均
有统计学意义(P<0.05)。结论:0.1Hz、10%拉伸幅度的机械牵张力加载5d可使NSFB表现出HSFB的部分生化特征,机械牵
张力对NSFB向HSFB转化过程有明显的诱导作用。

Abstract:
Abstract: Objective To investigate the effect of mechanical stretch at different loading times on the transformation of
normal skin fibroblasts (NSFB) into hypertrophic scar fibroblasts (HSFB). Methods The same demic NSFB and HSFB were
respectively divided into the experimental groups and the control groups. The experimental groups were loaded with 0.1Hz,
10% amplitude cyclic stretch 3, 5 and 7 days. The control groups were cultured without cyclic stretch. CCK-8 method was
used to measure the cell proliferation. SYBR Green qPCR method was used to measure the expression of p130Crk-associated
substance (p130Cas) , Integrin-β1, transforming growth factor β1 (TGF-β1) , type I collagen and Alpha-smooth muscle actin(α-
SMA) . ELISA method was used to measure the content of type I collagen and TGF-β1 in culture supernatant. Western-Blot
method was used to detect the content of protein Integrin-β1, p130Cas and α-SMA. Results With 5-days-long amplitude cyclic
stretch, cell proliferation and expression of p130Cas, Integrin-β1, TGF-β1, α-SMA and type I collagen of the NSFB experiment
group showed no significant difference with that of the HSFB control group(P >0.05). With 3-days-long and 7-days-long
amplitude cyclic stretch, the above indexes showed significant differences (P <0.05). Conclusion The mechanical tension
loading of 0.1Hz and 10% tensile amplitude 5d can make NSFB exhibit some biochemical characteristics of HSFB, and the
mechanical tension can obviously induce the transformation of NSFB to HSFB.

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更新日期/Last Update: 2018-08-08