[1]楚菲菲,王大雷,闫 伦,等.阻断Notch信号的角质形成细胞对成纤维细胞增殖及分泌功能的影响研究[J].中国美容医学,2018,(08):73-77.
　CHU Fei-fei,WANG Da-lei,YAN Lun,et al.Differences of Cell Proliferation and Secretion in Fibroblasts Co-culture with Notch Signaling Occured Keratinocytes[J].Medical Aesthetics and Beauty,2018,(08):73-77.
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阻断Notch信号的角质形成细胞对成纤维细胞增殖及分泌功能的影响研究
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2018年08期
页码:
73-77
栏目:
出版日期:
2018-09-10

文章信息/Info

Title:
Differences of Cell Proliferation and Secretion in Fibroblasts Co-culture with Notch Signaling Occured Keratinocytes
文章编号:
1008-6455（2018）08-0073-05
作者:
楚菲菲; 王大雷; 闫 伦; 李辉超; 夏 炜; 马显杰
Author(s):
CHU Fei-fei; WANG Da-lei; YAN Lun; LI Hui-chao; XIA Wei; MA Xian-jie
关键词:
[关键词]Notch信号; 角质形成细胞; 成纤维细胞; 复合培养模型; KGF; TGF-β1
Keywords:
Key words: Notch signaling;  keratinocyte;  fibroblast;  co-culture;  KGF;  TGF-β1
分类号:
R329
文献标志码:
A
摘要:

[摘要]目的：探讨在复合培养的条件下，将阻断Notch信号后的角质形成细胞与成纤维细胞共培养，对成纤维细胞增殖及表达
角质细胞生长因子（Keratinocyte growth factor，KGF）、TGF-β1的影响。方法：运用角质形成细胞-成纤维细胞复合培养的
模型，于复合培养前3d进行血清刺激或者γ-分泌酶抑制剂（DAPT）阻断角质形成细胞中的Notch信号，即在角质形成细胞分化
前进行干预。然后提升至气液交界面使其达到复层生长和终末分化，后与成纤维细胞共培养，观察阻断Notch信号后的角质形
成细胞对成纤维细胞增殖及分泌功能的影响。结果：分化前，给予角质形成细胞血清刺激，复合培养0h，Notch-1、Jagged-1
表达明显升高（P ＜0.05）；复合培养第1天，P21表达上调、P63表达下降（P ＜0.05）。血清刺激前给予DAPT预处理，复合培
养0h，角质形成细胞中Notch信号下游分子P21和P63的表达恢复至无血清刺激水平（P ＜0.05）。DAPT组的成纤维细胞增殖能
力、KGF及TGF-β1的表达相对于血清刺激组明显被抑制（P＜0.05），而与无血清组比较，差异无统计学意义（P ＞0.05）。结
论：阻断Notch信号后的角质形成细胞与成纤维细胞共培养，能够明显抑制成纤维细胞的增殖及KGF、TGF-β1的合成。

Abstract:
Abstract: Objective To study the impact of cell proliferation and expression of KGF and TGF-β1 in fibroblasts which are coculturing
with Notch signaling occurred keratinocytes. Methods To use keratinocytes which were stimulated by serum or
pretreated by DAPT (inhibitor of γ-secretase) to block Notch signaling(before the terminal differentiation in keratinocytes) cocultured
with fibroblasts, and detect the impact of cell proliferation and expression of KGF and TGF-β1 in fibroblasts. Results
To stimulate the keratinocytes with 10% FCS before the terminal differentiation, the expression of Notch-1 and Jagged-1
up-regulated at 0 hour of co-culture(P ＜0.05). And the expression of downstream gene P21 increased, but P63 decreased
on the first day of co-culture(P ＜0.05). After pretreatment with DAPT before serum stimulation, the expression of P21 and
P63 downstream of Notch signal in keratinocytes returned to serum-free level after 0h of co-culture(P ＜0.05). And the cell
proliferation and expression of KGF and TGF-β1 in fibroblasts of DAPT group had significant decreased compared to serumstimulated
group(P ＜0.05), but almost no differences with serum-free group(P ＞0.05). Conclusion When keratinocytes coculturing
with fibroblasts, blocking Notch signaling pathway in keratinocyte could suppress the cell proliferation and expression
of KGF and TGF-β1 in fibroblasts

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更新日期/Last Update: 2018-10-12