[1]吴文伯,陈敏亮,吴焱秋,等. 富血小板血浆（PRP）对大鼠光老化皮肤胶原蛋白、TGF-β及MMP-1表达的影响[J].中国美容医学,2019,(04):64-67.
　WU Wen-bo,CHEN Min-liang,WU Yan-qiu,et al. Effect of Platelet-rich Plasma (PRP) on the Expression of Collagen, TGF-β and MMP-1 in Photo-aged Skin of Rat[J].Medical Aesthetics and Beauty,2019,(04):64-67.
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 富血小板血浆（PRP）对大鼠光老化皮肤胶原蛋白、TGF-β及MMP-1表达的影响
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2019年04期
页码:
64-67
栏目:
出版日期:
2019-04-15

文章信息/Info

Title:
 Effect of Platelet-rich Plasma (PRP) on the Expression of Collagen, TGF-β and MMP-1 in Photo-aged Skin of Rat
文章编号:
1008-6455（2019）04-0064-04
作者:
吴文伯; 陈敏亮; 吴焱秋; 李光磊
Author(s):
WU Wen-bo; CHEN Min-liang; WU Yan-qiu; LI Guang-lei
关键词:
[关键词]富血小板血浆; 光老化; Ⅰ型胶原; Ⅲ型胶原; TGF-β; MMP-1
Keywords:
Key words: platelet-rich plasma(PRP);  photoaging;  type Ⅰ collagen;  type Ⅲ collagen;  TGF-β;  MMP-1
分类号:
R758.1
文献标志码:
A
摘要:
[摘要]目的：通过测定注射富血小板血浆（Platelet-rich plasma，PRP）后大鼠光老化皮肤Ⅰ、Ⅲ型胶原蛋白，转化因子β
（TGF-β），基质金属蛋白酶1（MMP-1）的表达量，观察PRP对大鼠光老化皮肤的改善作用。方法：通过紫外线照射大鼠背部
皮肤建立皮肤光老化模型。将模型动物随机分为5组，A-PRP注射组注射激活的PRP（A-PRP）；N-PRP注射组注射未激活的PRP
（N-PRP）；生理盐水注射组注射生理盐水；空白对照组仅照射建模；正常大鼠组仅剃除毛发。干预4周后，通过免疫组化法
对大鼠皮肤Ⅰ、Ⅲ型胶原蛋白含量进行半定量分析；通过Western bolt法对大鼠皮肤TGF-β、MMP-1表达量进行测定。结果：
Ⅰ型胶原表达：A-PRP注射组较N-PRP注射组、生理盐水注射组、空白对照组显著升高，差异有统计学意义（P ＜0.05）。Ⅲ
型胶原表达：A-PRP注射组显著高于生理盐水注射组、空白对照组（P ＜0.05），与N-PRP注射组比较，差异无统计学意义
（P ＞0.05）。TGF-β表达：A-PRP注射组显著高于N-PRP注射组、生理盐水注射组、空白对照组（P ＜0.05）；MMP-1表达：
A-PRP注射组显著低于生理盐水注射组、空白对照组（P ＜0.05），与N-PRP注射组比较，差异无统计学意义（P ＞0.05）。结
论：注射PRP可以提高光老化大鼠皮肤Ⅰ、Ⅲ型胶原蛋白和TGF-β表达，降低MMP-1表达，其中A-PRP对Ⅰ型胶原和TGF-β表
达的促进作用优于N-PRP。

Abstract:
Abstract: Objective The effect of PRP on photoaging skin of mice was observed by measuring the expression of type Ⅰand
type Ⅲ collagen, TGF-β, MMP-1 in the skin of mice after PRP injection. Methods Skin photoaging model was established by
irradiating the skin of the back of rats with UV light. A-PRP injection group was injected with activated PRP (A-PRP). N-PRP
injection group was injected with inactive PRP (N-PRP). Saline injection group was injected with normal saline. Blank control
group was irradiated only with UV. Normal rat group only shave the hair. After injection for 4 weeks, the content of collagen
type Ⅰ and Ⅲ in rat skin was semi-quantitatively analyzed by immunohistochemistry. The expression of TGF-β and MMP-1 in
rat skin was determined by Western blot. Results The expression of type Ⅰ collagen in A-PRP injection group was significantly
higher than that in N-PRP injection group, saline injection group and blank control group, the differences were statistically
significant(P ＜0.05). The expression of type Ⅲ collagen in A-PRP injection group was significantly higher than that in group
saline injection group and blank control group(P ＜0.05), and there was no significant difference between A-PRP injection group
and N-PRP injection group(P ＞0.05). The expression of TGF-β in A-PRP injection group was significantly higher than that in
N-PRP injection group, saline injection group and blank control group(P ＜0.05), the expression of MMP-1 was significantly
lower in A-PRP injection group than that in saline injection group and blank control group(P ＜0.05). The expression of MMP-1
in A-PRP injection group and N-PRP injection group had no statistical significance(P ＞0.05). Conclusion Injection of PRP can
enhance the expression of type Ⅰ collagen, type Ⅲ collagen and TGF-β and decrease the expression of MMP-1 in the photoaged
rat skin. The effect of A-PRP on the expression of type Ⅰ collagen and TGF-β is better than that of N-PRP.

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更新日期/Last Update: 2019-05-05