[1]郑舒丹,程诗萌,董亚兵,等. GNB2L1对恶性黑素瘤细胞增殖和迁移的影响[J].中国美容医学,2019,(06):59-63.
　ZHENG Shu-dan,CHENG Shi-meng,DONG Ya-bing,et al. Effects of GNB2L1 on Proliferation and Migration of Malignant Melanoma Cells[J].Medical Aesthetics and Beauty,2019,(06):59-63.
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 GNB2L1对恶性黑素瘤细胞增殖和迁移的影响
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2019年06期
页码:
59-63
栏目:
出版日期:
2019-06-05

文章信息/Info

Title:
 Effects of GNB2L1 on Proliferation and Migration of Malignant Melanoma Cells
文章编号:
1008-6455（2019）06-0059-05
作者:
郑舒丹1; 程诗萌2; 董亚兵2; 李孟森3; 刘天一4; 朱宁文1; 2
Author(s):
 ZHENG Shu-dan1; CHENG Shi-meng2; DONG Ya-bing2; LI Meng-sen3; LIU Tian-yi4; ZHU Ning-wen1; 2
关键词:
[关键词]GNB2Ll; 恶性黑素瘤; shRNA; 上皮间质转化; 增殖; 迁移
Keywords:
Key words: GNB2Ll;  malignant melanoma;  shRNA;  EMT;  proliferation;  migration
分类号:
R739.5
文献标志码:
A
摘要:
[摘要]目的：探讨支架蛋白重组人鸟嘌呤核苷酸结合蛋白beta多肽2样蛋白1（guanine nucleotide-binding protein
subunit Beta 2-like 1，GNB2L1）对小鼠恶性黑素瘤B16F10细胞增殖和迁移的影响。方法：用靶向GNB2Ll的短发夹RNA
（short hairpin RNA，shRNA）慢病毒液和空载体慢病毒液感染恶性黑素瘤B16F10细胞，分别获得稳定感染细胞株B16F10-
GNB2L1-shRNA（阳性实验组）和B16F10-NC（阴性对照组），未感染病毒液的细胞株B16F10设为空白对照组。Western blot
检测GNB2Ll以及上皮间质转化标志蛋白E-钙黏蛋白（E-cadherin）和N-钙黏蛋白（N-cadherin）的表达水平；CCK-8检测细
胞的增殖能力；划痕实验检测细胞的迁移能力。结果：与B16F10和B16F10-NC相比，B16F10-GNB2L1-shRNA中GNB2Ll蛋白表达
水平明显下调；GNB2L1敲降可明显抑制B16F10细胞的增殖和划痕迁移能力；Western blot特异性抗体检测发现GNB2L1敲降的
细胞中N-cadherin的蛋白表达水平明显降低，而E-cadherin的蛋白表达水平明显升高。结论：下调GNB2Ll蛋白表达可有效抑
制恶性黑素瘤B16F10细胞的增殖和迁移能力。

Abstract:
Abstract: Objective To investigate the effect of scaffold protein Guanine nucleotide-binding protein subunit Beta 2-like 1
(GNB2L1) on proliferation and migration of mouse malignant melanoma B16F10 cells. Methods Malignant melanoma B16F10
cells were infected with short hairpin RNA (shRNA) lentiviral solution and empty vector lentiviral solution targeting GNB2L1
to obtain stable infected cell line B16F10-GNB2L1-shRNA (positive experimental group) and B16F10-NC (negative control
group), cell line B16F10 not infected with virus solution was set as a blank control group. Western blot was used to detect the
expression levels of GNB2L1 and epithelial-mesenchymal transition marker proteins E-cadherin and N-cadherin; CCK-8 was
used to detect the cells proliferation ability; scratch test was used to detect cells migration ability. Results Compared with
B16F10 and B16F10-NC, the expression of GNB2L1 protein was down-regulated in B16F10-GNB2L1-shRNA; GNB2L1
knockdown significantly inhibited the proliferation and scratch migration ability of B16F10 cells; Western blot specific antibody
detection found that GNB2L1 knockdown the expression level of N-cadherin protein was significantly decreased in cells, while
the expression level of E-cadherin protein was significantly increased. Conclusion Down-regulation of GNB2L1 protein
expression can effectively inhibit the proliferation and migration of malignant melanoma B16F10 cells.

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[1] 李 越 综述,刘 浥 吴信峰 审校. 先天性巨痣的治疗进展[J].中国美容医学,2020,(6):181.
　 LI Yue,LIU Yi,WU Xin-feng. Progress of Treatment on Giant Congenital Melanocytic Nevus[J].Medical Aesthetics and Beauty,2020,(06):181.
[2] 郎中亮,钟晓红,汪 凯,等. 负压创面治疗技术在恶性黑素瘤腹股沟淋巴结清扫术中的应用价值[J].中国美容医学,2020,(8):72.
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更新日期/Last Update: 2019-07-03