[1]薛 梅,张德利,刁庆春,等. 丹参酚酸B对过氧化氢引起的黑素细胞凋亡的保护作用研究[J].中国美容医学,2019,(06):64-66.
　XUE Mei,ZHANG De-li,DIAO Qing-chun,et al. Study on Magnesium Tanshinoate B Alleviated H2O2-induced Apoptosis in Human Epidermal Melanocytes[J].Medical Aesthetics and Beauty,2019,(06):64-66.
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 丹参酚酸B对过氧化氢引起的黑素细胞凋亡的保护作用研究
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2019年06期
页码:
64-66
栏目:
出版日期:
2019-06-05

文章信息/Info

Title:
 Study on Magnesium Tanshinoate B Alleviated H2O2-induced Apoptosis in Human Epidermal Melanocytes
文章编号:
1008-6455（2019）06-0064-03
作者:
薛 梅1; 张德利1; 刁庆春1; 涂彩霞2; 林 茂1
Author(s):
XUE Mei1; ZHANG De-li1; DIAO Qing-chun1; TU Cai-Xia2; LIN Mao1
关键词:
 ; [关键词]丹参酚酸B; 过氧化氢; 黑素细胞; 凋亡; 氧化应激; 白癜风
Keywords:
 ; Key words: magnesium tanshinoate B;  H2O2;  melanocyte;  apoptosis;  oxidative stress;  vitiligo
分类号:
R329.2+8
文献标志码:
A
摘要:
[摘要]目的：研究丹参酚酸B对过氧化氢（H2O2）诱导的人表皮黑素细胞氧化应激及凋亡的保护作用。方法：采用MTT法检测
H2O2对黑素细胞的活力抑制作用及丹参酚酸B的保护作用；Annexin-V/PI双染流式细胞术检测H2O2诱导黑素细胞凋亡的作用及
丹参酚酸B的保护作用；DCFH-DA法流式细胞术检测H2O2促进黑素细胞活性氧簇（ROS）生成的作用及丹参酚酸B的抗氧化效
果。结果：250μmol/L H2O2作用于黑素细胞24h可明显降低细胞活力、增加凋亡。0.6～10μmol/L丹参酚酸B预处理1h可明
显保护黑素细胞，减少H2O2诱导的黑素细胞活力降低及凋亡。H2O2作用2h可显著诱导ROS生成，而丹参酚酸B可显著抑制H2O2
诱导的ROS生成。结论：丹参酚酸B可能抑制活性氧生成，减少H2O2引起的黑素细胞凋亡，因而可能具有成为治疗白癜风新药
的前景。
Abstract:
Abstract: Objective To test the effects of magnesium tanshinoate B on H2O2-induced oxidative stress and apoptosis in human
epidermal melanocytes. Methods Effects of H2O2 and magnesium tanshinoate B on cell viability were tested by MTT assay.
The apoptosis of melanocytes were detected by annexin-V/PI staining cytometry analysis. ROS generation was measured
by cytometry analysis with DCFH-DA method. Results The cell viability of melanocytes deceased and apoptosis rate and
ROS generation increased after treated with 250μmol/L H2O2 for 24h, while pretreatment with 0.6～10μmol/L magnesium
tanshinoate B significantly alleviated the effects of H2O2. Conclusions Magnesium tanshinoate B alleviated H2O2-induced
apoptosis in melanocytes probably by inhibiting the ROS generation according to its antioxidant ability, thus may have the
potential to be used in treating vitiligo in the future.

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更新日期/Last Update: 2019-07-03