[1]任 捷,马 莉,严淑贤. 杜仲提取液对紫外线照射下真皮成纤维细胞保护作用的研究[J].中国美容医学,2019,(10):99-101.
　REN Jie,MA Li,YAN Shu-Xian. Protective Effects of Eucommia Ulmoides Oliver Extracts on Ultraviolet Irradiated Human Dermal Fibroblasts[J].Medical Aesthetics and Beauty,2019,(10):99-101.
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 杜仲提取液对紫外线照射下真皮成纤维细胞保护作用的研究
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2019年10期
页码:
99-101
栏目:
出版日期:
2019-10-05

文章信息/Info

Title:
 Protective Effects of Eucommia Ulmoides Oliver Extracts on Ultraviolet Irradiated Human Dermal Fibroblasts
文章编号:
1008-6455（2019）10-0099-04
作者:
任 捷; 马 莉; 严淑贤
Author(s):
REN Jie; MA Li; YAN Shu-Xian
关键词:
 ; [关键词]紫外线; 皮肤衰老; 真皮; 成纤维细胞; 杜仲提取液
Keywords:
 ; Key words: ultraviolet rays;  skin aging;  dermis;  fibroblast;  Eucommia ulmoides oliver extracts
分类号:
R622
文献标志码:
A
摘要:
[摘要]目的：研究紫外线（UV）照射对真皮成纤维细胞功能的影响，观察杜仲提取液对紫外线照射下细胞的保护作用。方
法：体外培养人真皮成纤维细胞接收长波紫外线或中波紫外线照射，同时加入杜仲提取液，检测细胞存活率，基质金属蛋白
酶（MMP）-1和3的mRNA表达，以及Ⅰ型、Ⅲ型前胶原蛋白表达水平。结果：与未照射组相比，20J/cm2 UVA1和40mJ/cm2 UVB
照射组可显著抑制细胞存活率、增加MMP-1和MMP-3 mRNA水平、抑制Ⅰ型和Ⅲ型前胶原蛋白表达水平（P ＜0.01）。照射同时
加入1mg/ml杜仲提取液可以显著减少UVA1或UVB照射对细胞引起的上述改变（P ＜0.05）。结论：杜仲提取液对UV照射所致真
皮成纤维细胞的增殖损伤、胶原合成和分解代谢紊乱有保护作用，因而可以应用于防治皮肤光老化。

Abstract:
Abstract: Objective To determine the effects of Eucommia ulmoides oliver extracts on ultraviolet irradiated human dermal
fibroblasts in vitro. Methods Human dermal fibroblasts were irradiated by a single exposure to UVA1 or UVB and at the same
time incubated with, or without Eucommia ulmoides oliver extracts and then detected twenty-four hours later. Cell viability rate,
expressions of MMP-1, MMP-3 and procollagen Ⅰ, procollagen Ⅲ were detected. Results 20J/cm2 UVA1 or 40mJ/cm2UVB
significantly inhibited the cell viability rate and procollagen Ⅰ, procollagen Ⅲ protein levels (P ＜0.01), while stimulated
MMP-1 and MMP-3 mRNA expression (P ＜0.01). Eucommia ulmoides oliver extracts reversed these effects caused by UV
in some degree and in 1mg/ml concentration (P ＜0.05). Conclusion Eucommia ulmoides oliver extracts had photoprotective
properties against UV irradiation in vitro, which inhibited extracellular matrix degradation and preserved collagen production in
dermis, and might be used as an anti-photoaging agent.

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更新日期/Last Update: 2019-11-07