脉冲电磁场对新型低弹多孔钛合金表面成骨效应的影响研究<br />-《中国美容医学》

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Title:: Pulsed Electromagnetic Fields Enhance Biological Effects of Osteogenesis on Porous
Titanium Alloy Surface

文章编号:: 1008-6455（2020）06-0091-06

作者:: 刘文文¹; 杨巍¹; 吴智钢²; 石磊¹; 郭硕¹; 魏星辉¹; 汤臻¹; 王宁¹; 李小康¹; 郭征¹

Author(s):: LIU Wen-wen1; YANG Wei1; WU Zhi-gang2; SHI Lei1; GUO Shuo1; WEI Xing-hui1; TANG Zhen1; WANG Ning1; LI Xiao-kang1; GUO Zheng1

关键词:: [关键词]钛合金; 脉冲电磁场; 多孔材料; 骨整合; 成骨

Keywords:: Key words: titanium alloy; pulsed electromagnetic field; porous material; osseointegration; osteogenesis

分类号:: R68

文献标志码:: A

摘要:: [摘要]目的：观察脉冲电磁场刺激对新型低弹多孔钛合金支架表面的成骨效应。方法：使用3D打印技术制备多孔钛合金(Ti-
24Nb-4Zr-8Sn，Ti2448)支架,并使用扫描电镜对支架的表面形貌进行观察；将成骨细胞接种于支架表面，给予脉冲电磁场
刺激的支架为实验（PMEF）组，不给予脉冲电磁场刺激的支架为对照组；使用CCK-8(Cell Counting Kit-8)试剂盒检测两
组细胞的增殖能力；使用活/死细胞染色观察两组细胞在支架上的活性；使用扫描电镜观察两组细胞在支架上的形态；通
过实时定量PCR观察两组细胞相关成骨基因分化；通过兔股骨外侧髁缺损模型进行体内骨长入分析，分别使用荧光标记和
Van Gieson染色观察两组支架的骨长入情况。结果：使用3D打印技术成功制备实验所需的多孔钛合金支架；PMEF组成骨细
胞的增殖能力、细胞活性明显高于对照组，差异有统计学意义(P ＜0.05)；PMEF组成骨细胞可见明显细胞伪足，细胞状态良
好；PMEF组成骨细胞的成骨相关基因表达明显高于对照组(P ＜0.05)；术后4周和12周，PMEF组新生骨沉积速率（两种荧光间
距）均高于对照组(P ＜0.05)，Van Gieson染色发现PMEF组骨长入明显多于对照组(P ＜0.05)。结论：脉冲电磁场刺激和多孔
Ti2448植入物的组合可为骨科、口腔和整形等领域的骨修复重建提供一种新方法和新思路。

Abstract:: Abstract: Objective Observe the osteogenic effect of PEMF stimulation on the surface of the new low-elasticity porous
titanium alloy scaffold. Methods The new low-elasticity porous titanium alloy (Ti-24Nb-4Zr-8Sn, Ti2448) scaffolds were
prepared by 3D printing technology. The surface morphology of the scaffolds were characterized by scanning electron
microscopy (SEM). The osteoblast-like cell line MC3T3-E1 was cultured in the absence (control) or presence of PEMF
stimulation on porous Ti2448 disc surface, and the adhesion and proliferation of the cells were investigated by SEM, live/dead
cell imaging kit and CCK-8 assay. Furthermore, the expression of osteogenesis-related genes was also examined by quantitative
real-time PCR. The porous Ti2448 scaffolds for animal experiment were implanted into the lateral femoral epicondyle of female
New Zealand white rabbits. All of the 24 rabbits were then randomly divided equally into two groups, one group with PEMF
stimulation for 2 h everyday and the other with no stimulation as control. After 4 weeks and 12 weeks, fluorescent markers and
Van Gieson staining were used to observe the bone ingrowth of the two groups of scaffolds. Results The porous titanium alloy
scaffolds required for the experiment were successfully prepared. The proliferation ability and cell activity of MC3T3-E1 in
PMEF group were significantly higher than those in control group (P ＜0.05). The cells in the PMEF group showed obvious cell
pseudopodia, the osteoblast-related genes expression of cells in PMEF group were significantly higher than that in control group
(P ＜0.05). At 4 and 12 weeks after surgery, the rate of new bone deposition (two types of fluorescence spacing) in the PMEF
group was higher than that in the control group (P ＜0.05). Van Gieson staining showed that the bone growth in the PMEF group
was significantly more than that in the control group (P ＜0.05). Conclusion The combination of PMEF and porous Ti2448
implant can provide a new idea for bone repair and reconstruction in the fields of orthopedics, oral cavity and plastic surgery.

《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

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