[1] 林 简,刘晓红,王荣坤,等. MicroRNA-501-5p在瘢痕疙瘩中的表达及与eIF3L的靶向关系探讨[J].中国美容医学,2020,(11):93-96.
　 LIN Jian,LIU Xiao-hong,WANG Rong-kun,et al. Expression of MiR-501-5p in Keloid and its Targeting Correlatin with eIF3L[J].Medical Aesthetics and Beauty,2020,(11):93-96.
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 MicroRNA-501-5p在瘢痕疙瘩中的表达及与eIF3L的靶向关系探讨
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2020年11期
页码:
93-96
栏目:
出版日期:
2020-11-10

文章信息/Info

Title:
 Expression of MiR-501-5p in Keloid and its Targeting Correlatin with eIF3L
文章编号:
1008-6455（2020）11-0093-04
作者:
 林 简; 刘晓红; 王荣坤; 李 涛; 李 娜; 陈惠娟
Author(s):
 LIN Jian; LIU Xiao-hong; WANG Rong-kun; LI Tao; LI Na; CHEN Hui-juan
关键词:
 [关键词]瘢痕疙瘩; 永生化成纤维细胞系; 微小RNA-501-5p; 真核翻译起始因子3L; 靶向关系; 调控
Keywords:
 Key words: keloid;  immortalized fi broblast cell line;  microRNA-501-5p;  eukaryotic translation initiation factor 3L;  targetingrelationship;  regulation
分类号:
R619+.6
文献标志码:
A
摘要:
[摘要]目的：检测瘢痕疙瘩中MicroRNA-501-5p（miR-501-5p）的表达，分析其与真核翻译起始因子3L（eIF3L）的靶向关
系。方法：选择瘢痕疙瘩组织41例作为观察组，选择增生性瘢痕41例为对照组，选择正常皮肤组织41例作为正常对照组。应用
实时荧光定量PCR法（qPCR）检测3组中miR-501-5p的表达，应用免疫组化法检测瘢痕疙瘩中eIF3L和Ki67的表达。选择永生化成
纤维细胞系，应用双荧光素酶报告基因实验观察miR-501-5p与eIF3L的结合情况。结果：三组中miR-501-5p的表达比较差异有统
计学意义（P＜0.05）。观察组中miR-501-5p的表达在有无家族史及不同病程的分组中差异有统计学意义（P ＜0.05）。相关分
析显示miR-501-5p与eIF3L呈正相关性。双荧光素酶报告基因实验显示miR-501-5p与eIF3L具有靶向关系。结论：瘢痕疙瘩中
miR-501-5p高表达，与家族史及病程相关。miR-501-5p与eIF3L具有靶向关系。

Abstract:
Abstract: Objective To detect the expression of microRNA-501-5p(miR-501-5p) in keloid, analyze its relationship with
eukaryotic translation initiation factor 3L(eIF3L). Methods 41 cases of keloid tissue were selected as observation group. 41
cases of hypertrophic scar were selected as control group. 41 cases of normal skin tissue were selected as normal control group.
Expression of miR-501-5p was detected by real-time fl uorescent quantitative PCR(qPCR) in three groups. EIF3L and Ki67
were detected by immunohistochemistry in keloid. Immortalized fi broblast cell line was selected. The binding of miR-501-5p
and eIF3L was observed by double luciferase reporter gene experiment. Results Expression of miR-501-5p was signifi cantly
different in three groups (P ＜0.05). Expression of miR-501-5p was statistically signifi cant in family history and different course
in the observation group(P ＜0.05). Positive correlation was found between miR-501-5p and eIF3L. Targeting relationship was
found between miR-501-5p and eIF3L by double luciferase reporter gene experiment. Conclusion Higher expression of miR-
501-5p is related to family history and course of disease in keloid. MiR-501-5p has targeting relationship with eIF3L.

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更新日期/Last Update: 2020-11-24