[1] 李程彬,徐威龙,李 婷,等. DANCR通过AKT-GSK3β-Cyclin D1信号通路调控黑素瘤细胞活性机制的研究[J].中国美容医学,2021,(3):86-90.
　 LI Cheng-bin,XU Wei-long,LI Ting,et al. DANCR was Upregulated in Melanoma Tissues and Promoted Cell Viability Via AKTGSK3β-Cyclin D1 Signaling[J].Medical Aesthetics and Beauty,2021,(3):86-90.
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 DANCR通过AKT-GSK3β-Cyclin D1信号通路调控黑素瘤细胞
活性机制的研究
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2021年3期
页码:
86-90
栏目:
出版日期:
2021-03-10

文章信息/Info

Title:
 DANCR was Upregulated in Melanoma Tissues and Promoted Cell Viability Via AKTGSK3β-
Cyclin D1 Signaling
文章编号:
1008-6455（2021）03-0086-04
作者:
 李程彬; 徐威龙; 李 婷; 陈 佳; 舒茂国; 贾 晶
Author(s):
 LI Cheng-bin; XU Wei-long; LI Ting; CHEN Jia; SHU Mao-guo; JIA Jing
关键词:
 [关键词]LncRNA DANCR; 黑素瘤; 细胞活性; 增殖; AKT; GSK3β; Cyclin D1
Keywords:
 Key words: LncRNA DANCR;  melanoma;  cell viability;  proliferation;  AKT;  GSK3β;  Cyclin D1
分类号:
R739.5
文献标志码:
A
摘要:
[摘要]目的：探索长链非编码（Long non-coding，Lnc）RNA DANCR(Anti-differentiation nc RNA)在黑素瘤组织中表达
及其对黑素瘤细胞的作用及其作用机制。方法：检测黑素瘤和瘤旁组织中DANCR表达，及其与患者临床预后的关系。在敲
低DANCR的黑素瘤细胞株A375和B16中通过MTT和克隆形成实验检测敲低DANCR对细胞活性影响，通过Western-blot实验检测
p-AKT，p-GSK3β和Cyclin D1表达，并通过细胞流式检测细胞周期分布。通过过表达DANCR检测其是否通过AKT/GSK3β调控
细胞活性。结果：DANCR在黑素瘤组织中表达上调，并与黑素瘤不良临床预后相关；敲低DANCR可抑制黑素瘤细胞活性，下调
p-AKT、p-GSK3β和Cyclin D1表达；抑制AKT或GSK3β均可减弱DANCR过表达对细胞活性上调作用。结论：DANCR在黑素瘤中
表达上调，激活AKT-GSK3β-Cyclin D1信号通路上调细胞活性，促进黑素瘤进展。

Abstract:
Abstract: Objective To investigate the expression of long non-coding RNA DANCR in melanoma tissues, and the role and
potential mechanism of DANCR in melanoma development. Methods Detecting the expression of DANCR in melanoma and
the adjacent tissues to analyze the relation of DANCR expression and clinical prognosis. Knocking DANCR down in melanoma
cell lines, A375 and B16. Cell viability was detected using MTT and colony formation assays. Western blot assay detected
the expression of p-AKT, p-GSK3β and Cyclin D1. Flow cytometry assay analyzed the distribution of cell cycle. DANCRoverexpressed
cells were used to confirm that DANCR regulated cell viability via AKT/ GSK3β signaling. Results DANCR
expression was upregulated in melanoma tissues compared with the adjacent tissues, which was associated with poor clinical
prognosis. Knocking DANCR down repressed cell viability and diminished expression of p-AKT, p-GSK3β and Cyclin D1.
Inhibiting both p-AKT and p-GSK3β reversed DANCR overexpression induced enhancement of cell viability. Conclusion
DANCR was upregulated in melanoma cells and enhanced melanoma cell viability via modulating AKT-GSK3β-Cyclin D1
signaling.

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更新日期/Last Update: 2021-04-13