[1] 李泰平,肖 红. 木犀草素对兔耳增生性瘢痕抑制作用机制的初步研究[J].中国美容医学,2021,(8):91-94.
  LI Tai-ping,XIAO Hong. Preliminary Study on the Inhibitory Mechanism of Luteolin on Hypertrophic Scar inRabbit Ear[J].Medical Aesthetics and Beauty,2021,(8):91-94.
点击复制

 木犀草素对兔耳增生性瘢痕抑制作用机制的初步研究
()
分享到:

《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2021年8期
页码:
91-94
栏目:
出版日期:
2021-08-10

文章信息/Info

Title:
 Preliminary Study on the Inhibitory Mechanism of Luteolin on Hypertrophic Scar in
Rabbit Ear
文章编号:
1008-6455(2021)08-0091-04
作者:
 李泰平肖 红
Author(s):
 LI Tai-pingXIAO Hong
关键词:
 [关键词]木犀草素增生性瘢痕兔耳模型机制研究
Keywords:
 Key words: luteolin hypertrophic scar rabbit ear model mechanism research
分类号:
R619+.6
文献标志码:
A
摘要:
[摘要]目的:初步研究木犀草素对兔耳增生性瘢痕的抑制作用及可能机制。方法:9只新西兰兔随机分为空白组、生理盐水
对照组和药物干预组,每组3只,生理盐水对照组和药物干预组建立兔耳增生性瘢痕模型,每只兔左右耳各3个直径约0.7cm
瘢痕创面,相距约1cm。生理盐水对照组给予生理盐水,药物干预组给予木犀草素乳膏,瘢痕组织于给药前和给药后40d取
材,Masson染色后显微镜下观察各组瘢痕组织的病理变化,ELISA法检测瘢痕组织中转化生长因子-β(TGF-β)、血小板
衍化生长因子(PDGF-BB)、结缔组织生长因子(CTGF)的含量,qRT-PCR和Western Blot法检测TGF-β、Ⅰ型胶原蛋白
(Collagen Ⅰ)和基质金属蛋白酶-2(MMP-2)的表达水平。结果:Masson染色显示木犀草素干预后瘢痕组织中胶原沉积减
少,胶原纤维致密且排列规则。与生理盐水对照组相比,药物干预组瘢痕组织中TGF-β、PDGF-BB、CTGF的表达明显减少,
差异有统计学意义(P <0.05)。此外,qRT-PCR和Western Blot结果显示,与生理盐水对照组相比,药物干预组MMP-2表达
升高,而Collagen Ⅰ表达水平明显降低,差异有统计学意义(P <0.05)。结论:木犀草素能够抑制兔耳增生性瘢痕的形
成,其作用机制可能与抑制TGF-β、PDGF-BB、CTGF的分泌、上调MMP-2、减少Collagen Ⅰ的表达有关。

Abstract:
Abstract: Objective To reveal the inhibitory effect of luteolin and its mechanism on hypertrophic scar formation in rabbit
ears. Methods 9 New Zealand rabbits were randomly divided into 3 groups: the blank group, the normal saline control group
and the drug intervention group, with 3 rabbits in each group. Hypertrophic scar model was established in rabbit ears in the
normal saline control group and the drug intervention group. Before and forty days after treatment, the pathological changes
of the scar tissues were examined, and the collagen fibers hyperplasia were observed with Masson staining. The expression
levels of TGF-β, PDGF-BB, CTGF were quantified by ELISA. In addition, TGF-β, Collagen Ⅰand MMP-2 were
separately detected by quantitative real-time PCR (qRT-PCR) assay and Western Blot. Results Masson staining showed
that collagen deposition was significantly reduced after luteolin treatment, collagen fibers were also dense and arranged
regularly. ELISA results indicated that the expression levels of TGF-β, PDGF-BB and CTGF were obviously reduced in
the drug intervention group compared to the the normal saline control group, the differences were statistically significant
(P <0.05). In addition, the results of qRT-PCR and Western blot showed that compared with the normal saline control group,
the expression of MMP-2 in the drug intervention group increased, while the expression level of Collagen Ⅰ decreased
significantly (P <0.05). Conclusion Luteolin could inhibit the formation of hypertrophic scar in rabbit ears, and its mechanism
may be related to the inhibition of TGF-β、the secretion of PDGF-BB and CTGF was related to the up regulation of MMP-2
and the decrease of collagen Ⅰ expression in vivo.

相似文献/References:

[1]陈 方. 咪喹莫特调节Th1、Th2细胞相关趋化因子对兔耳增生性瘢痕创面愈合和瘢痕增生的影响[J].中国美容医学,2018,(03):100.
 CHEN Fang. Effect of Imiquimod Regulating Th1 and Th2 Cell-associated Chemokines on Wound Healing and Scar Proliferation in Rabbits[J].Medical Aesthetics and Beauty,2018,(8):100.
[2]黄显翔,张 伟,叶光荣,等. CO2点阵激光治疗增生性瘢痕的临床疗效及对TGF-β1表达的影响[J].中国美容医学,2018,(08):108.
 HUANG Xian-xiang,ZHANG Wei,YE Guang-rong,et al.Analysis of the Clinical Effects and Influence on Expression of TGF-β1 of CO2 Lattice Laser in the Treatment of Hypertrophic Scar[J].Medical Aesthetics and Beauty,2018,(8):108.
[3]谢 彬. 深度烧伤患者弹力绷带压力治疗对增生性瘢痕恢复情况的影响[J].中国美容医学,2018,(10):62.
 XIE Bin. Effect of Pressure Treatment with Elastic Bandage on Recovery of Hypertrophic Scar in Patients with Deep Burn[J].Medical Aesthetics and Beauty,2018,(8):62.
[4]俞 舜. 微等离子体射频联合曲安奈德超声导入治疗增生性瘢痕疗效分析[J].中国美容医学,2019,(02):63.
 YU Shun. Clinical Study on Microplasma Radiofrequency Combined with Ultrasonic ImportingTriamcinolone Acetonide in the Treatment of Hypertrophic Scar[J].Medical Aesthetics and Beauty,2019,(8):63.
[5]史 敏,李 娜,郭晓波,等.miR-21调控PTEN/PI3K/Akt通路抑制增生性瘢痕形成的机制研究[J].中国美容医学,2019,(03):92.
 SHI Min,LI Na,GUO Xiao-bo,et al. Mechanism of miR-21 Inhibiting Human Hypertrophic Scar Formation by RegulatingPTEN/PI3K/Akt Pathway[J].Medical Aesthetics and Beauty,2019,(8):92.
[6]李 丽,王 林. 正常皮肤与病理性瘢痕组织中HIF-1α和促炎细胞因子水平比较及两者相关性研究[J].中国美容医学,2019,(04):71.
 LI Li,WANG Lin. Levels of HIF-1α and Proinflammatory Cytokines in Normal Skin and PathologicalScar Tissue and Their Correlation[J].Medical Aesthetics and Beauty,2019,(8):71.
[7]史 敏 李 娜 陈 雷 刘兆旺 魏超鹏综述,封兴华审校. 自噬、miRNA与增生性瘢痕的研究进展[J].中国美容医学,2019,(04):160.
 SHI Min,LI Na,CHEN Lei,et al. Advance in Research on Autophagy, miRNA and Hypertrophic Scar[J].Medical Aesthetics and Beauty,2019,(8):160.
[8]吴志贤,李 响,莫自增,等. 异泽兰黄素通过PDGFβ/ERK信号通路抑制增生性瘢痕生长的机制探讨[J].中国美容医学,2019,(07):44.
 WU Zhi-xian,LI Xiang,MO Zi-zeng,et al.Eupatilin Inhibits Hypertrophic Scar Proliferation Via the PDGFβ/ERK Signaling Pathway[J].Medical Aesthetics and Beauty,2019,(8):44.
[9]伍星蓉,周平华,吴志强,等. 皮损内注射曲安奈德联合CO2点阵激光治疗增生性瘢痕[J].中国美容医学,2019,(09):32.
 WU Xing-rong,ZHOU Ping-hua,WU Zhi-qiang,et al. Intradermic Injection Triamcinolone Acetonide Combined with CO2 Dot Array Laserin the Treatment of Hypertrophic Scar[J].Medical Aesthetics and Beauty,2019,(8):32.
[10]李山林,陈小君,杨海微,等. 曲安奈德辅助激光治疗增生性瘢痕的疗效及对TGF-β1的影响[J].中国美容医学,2019,(12):66.
 LI Shan-lin,CHEN Xiao-jun,YANG Hai-wei,et al. Therapeutic Effect and the Effect of TGF-β1 of Patients with Hypertrophic Scar UsingTriamcinolone Acetonide-assisted Laser Therapy[J].Medical Aesthetics and Beauty,2019,(8):66.

更新日期/Last Update: 2021-09-10