[1]林 颜,阮树斌,陈晓东,等.干扰hsa_circ_0013958/miR-637对人瘢痕疙瘩成纤维细胞增殖、迁移和侵袭的影响[J].中国美容医学,2024,(4):54-58.
　LIN Yan,RUAN Shubin,CHEN Xiaodong,et al.Effect of Interference hsa_circ_0013958/miR-637 on the Proliferation, Migration and Invasion of Human Keloid Fibroblasts[J].Medical Aesthetics and Beauty,2024,(4):54-58.
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干扰hsa_circ_0013958/miR-637对人瘢痕疙瘩成纤维细胞增殖、迁移和侵袭的影响()
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2024年4期
页码:
54-58
栏目:
出版日期:
2024-04-10

文章信息/Info

Title:
Effect of Interference hsa_circ_0013958/miR-637 on the Proliferation, Migration and Invasion of Human Keloid Fibroblasts
文章编号:
1008-6455（2024）04-0054-05
作者:
林 颜; 阮树斌; 陈晓东; 杨荣华; 王婧薷; 林泽鹏; 信 琪
(佛山市第一人民医院烧伤整形创面修复外科 广东 佛山 528000 )
Author(s):
LIN Yan;  RUAN Shubin;  CHEN Xiaodong;  YANG Ronghua;  WANG Jingru;  LIN Zepeng;  XIN Qi
(Department of Burn Plastic Wound Repair Surgery, the First People’s Hospital of Foshan, Foshan 528000, Guangdong, China)
关键词:
hsa_circ_0013958; miR-637; 瘢痕疙瘩; 成纤维细胞; 增殖; 迁移; 侵袭
Keywords:
hsa_circ_0013958;  miR-637;  keloids;  fi broblasts;  proliferation;  migration;  invasion
分类号:
R619+ .6
文献标志码:
A
摘要:
目的：探讨环状RNA（circular RNA, circRNA）hsa_circ_0013958对人瘢痕疙瘩成纤维细胞（Human keloid fibroblast, HKF）增殖、迁移、侵袭的影响及分子机制。方法：将人瘢痕疙瘩成纤维细胞分为si-NC组、si-hsa_ circ_0013958组、miR-NC组、miR-637组、si-hsa_circ_0013958+anti-miR-NC组和si-hsa_circ_0013958+anti-miR-637组。 实时荧光定量PCR（Real time quantitative polymerase chain reaction, RT-qPCR）试剂盒检测hsa_circ_0013958、miR637表达；四甲基偶氮唑盐（Methyl thiazolyl tetrazolium, MTT）比色法检测细胞存活率；平板克隆实验检测细胞集落形 成数；Transwell检测细胞迁移和侵袭能力；双荧光素酶报告实验检测hsa_circ_0013958和miR-637的靶向关系。结果：瘢痕 疙瘩组织中hsa_circ_0013958表达水平较正常皮肤组织升高，miR-637表达水平降低（P＜0.05）。干扰hsa_circ_0013958 表达或过表达miR-637后，HKF细胞存活率、集落形成数以及迁移、侵袭细胞数均降低（P＜0.05）。hsa_circ_0013958和 miR-637 有靶向调控关系；抑制miR-637表达逆转了干扰hsa_circ_0013958对HKF增殖、迁移侵袭的影响。结论：干扰hsa_ circ_0013958通过调控miR-637抑制人瘢痕疙瘩成纤维细胞的增殖、迁移和侵袭。
Abstract:
Objective To explore the eff ect of circular RNA ( circRNA) hsa_circ_0013958 on the proliferation, migration and invasion of human keloid fibroblasts and its molecular mechanism. Methods Human keloid fibroblasts HKF were divided into si-NC group, si-hsa_circ_0013958 group, miR-NC group, miR-637 group, si-hsa_circ_0013958+anti-miR-NC group, sihsa_circ_0013958+anti-miR-637 group. Expression levels of hsa_circ_0013958 and miR-637 were detected by Real time quantitative polymerase chain reaction (RT-qPCR). Cell viability was assayed by Methyl thiazolyl tetrazolium (MTT) method, cell colony formation was detected by plate cloning test, cell migration and invasion ability was detected by transwell method, the targeting relationship between hsa_circ_0013958 and miR-637 was detected by dual luciferase reporter experiment. Results Compared with normal skin tissues, the expression level of hsa_circ_0013958 were elevated in keloid tissue, and the expression of miR-637 was decreased (P ＜0.05). After interference with hsa_circ_0013958 expression or overexpression of miR-637, the survival rate, colony forming number, migration and invasion of HKF cells were decreased (P＜0.05). Hsa_circ_0013958 has a targeted regulatory relationship with miR-637, inhibition of miR-637 expression reversed the eff ect of interference with hsa_circ_0013958 on the proliferation, migration and invasion of HKF. Conclusion Interference with hsa_circ_0013958 may inhibit the proliferation, migration and invasion of human keloid fi broblasts by regulating miR-637.
更新日期/Last Update: 2024-04-12