[1]张义林,侯 旭,汪 莉,等.miR-130a-5p靶向Runx2对牙槽骨成骨细胞增殖和分化的影响及其调控机制研究[J].中国美容医学,2024,(6):51-55.
　ZHANG Yilin,HOU Xu,WANG Li,et al.Effects of miR-130a-5p Targeting Runx2 on the Proliferation and Differentiation of Alveolar Bone Osteoblasts and Its Regulatory Mechanism[J].Medical Aesthetics and Beauty,2024,(6):51-55.
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miR-130a-5p靶向Runx2对牙槽骨成骨细胞增殖和分化的影响及其调控机制研究()
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2024年6期
页码:
51-55
栏目:
出版日期:
2024-06-10

文章信息/Info

Title:
Effects of miR-130a-5p Targeting Runx2 on the Proliferation and Differentiation of Alveolar Bone Osteoblasts and Its Regulatory Mechanism
文章编号:
1008-6455（2024）06-0051-05
作者:
张义林; 侯 旭; 汪 莉; 朗么磋; 李相宜
(四川大学华西空港医院·成都市双流区第一人民医院口腔科 四川 成都 610200 )
Author(s):
ZHANG Yilin;  HOU Xu;  WANG Li;  LANG Mecuo;  LI Xiangyi
( Department of Stomatology, West China Airport Hospital, Sichuan University, Chengdu Shuangliu First People’s Hospital, Chengdu 610200, Sichuan, China )
关键词:
miR-130a-5p; Runt相关转录因子2; 牙槽骨成骨细胞; Sonic hedgehog信号通路; 增殖; 分化; 调控机制
Keywords:
miR-130a-5p;  Runt-related transcription factor 2;  alveolar bone osteoblast;  Sonic hedgehog signal pathway;  proliferation;  diff erentiation;  regulatory mechanism
分类号:
R783.1
文献标志码:
A
摘要:
目的：探究miR-130a-5p靶向Runt相关转录因子2（Runt-related transcription factor 2，Runx2）对牙槽骨成骨细 胞（Alveolar osteoblast，AOB）增殖、凋亡、成骨分化的影响及其机制。方法：将AOB分为NC组、miR-NC组、miR-130a5p组、miR-130a-5p+Runx2组和miR-130a-5p+SHH组。双荧光素酶报告验证miR-130a-5p对Runx2、Sonic hedgehog（SHH）的 调控关系；CCK-8及EdU染色检测细胞增殖能力；AnnexinV-FITC/PI法检测细胞凋亡能力；ALP染色、茜素红染色检测细胞成 骨分化能力；RT-qPCR检测miR-130a-5p、Runx2、SHH、神经胶质瘤关联癌基因同源物1（Gli1）mRNA水平；Western Blot检 测增殖细胞核抗原（Proliferating cell nuclear antigen，PCNA）、Ki67、B细胞淋巴瘤/白血病-2（B-cell lymphoma/ leukemia-2，Bcl-2）、Bcl-2相关X蛋白（Bcl-2 related X protein，Bax）、碱性磷酸酶（Alkaline phosphatase， ALP ）、骨钙素（Osteocalcin，OCN）、骨形态发生蛋白2（Bone morphogenetic protein 2，BMP2）、Runx2、SHH、Gil1水 平。结果：miR-130a-5p靶向调控Runx2、SHH。过表达miR-130a-5p后，细胞24 h、48 h、72 h OD值及EdU阳性率降低，细胞 凋亡率升高，成骨分化能力降低，miR-130a-5p、Bax蛋白水平升高，Runx2、PCNA、Ki67、Bcl-2、ALP、OCN、BMP2蛋白及 SHH 、Gil1 mRNA和蛋白水平均降低（P＜0.05）。过表达miR-130a-5p和Runx2或过表达miR-130a-5p和SHH可减弱过表达miR130a-5p对细胞增殖、凋亡、成骨分化的影响。结论：miR-130a-5p靶向Runx2抑制AOB增殖和成骨分化，并促进AOB凋亡，其 可能通过抑制SHH信号通路发挥作用。
Abstract:
Objective To explore the eff ect of miR-130a-5p on proliferation, apoptosis and osteogenic diff erentiation of alveolar bone osteoblasts and its mechanism by targeting Runx2. Methods AOB was divided into NC group, miR-NC group, miR130a-5p group, miR-130a-5p+Runx2 group and miR-130a-5p+SHH group. The double luciferase report was used to verify the regulatory relationship of miR-130a-5p on Runx2 and SHH; CCK-8 and EdU staining were used to detect proliferation ability; Annexin V-FITC/PI method was used to detect apoptosis ability. ALP staining and alizarin red staining were used to detect the osteogenic diff erentiation ability. RT-qPCR was used to detect miR-130a-5p, Runx2, SHH and Gil1 mRNA levels. Western blot was used to detect PCNA, Ki67, Bcl-2, Bax, ALP, OCN, BMP2, Runx2, SHH, Gli1 protein levels. Results MiR-130a-5p targeted Runx2 and SHH. After overexpression of miR-130a-5p, OD value at 24 h, 48 h and 72 h and EdU positive rate of cells were decreased, and apoptosis rate was increased. The osteogenic diff erentiation ability was decreased, miR-130a-5p and Bax protein levels were increased, and Runx2, PCNA, Ki67, Bcl-2, ALP, OCN, BMP2 protein levels, SHH, Gil1 mRNA and protein levels were decreased (P ＜0.05). Overexpression of miR-130a-5p and Runx2 or overexpression of miR-130a-5p and SHH could attenuate the eff ect of overexpression of miR-130a-5p on proliferation, apoptosis and osteogenic diff erentiation ability. Conclusion MiR-130a-5p inhibits AOB proliferation and osteogenic diff erentiation, and promoted AOB apoptosis by targeting Runx2, which may play a role by inhibiting Sonic hedgehog signal pathway
更新日期/Last Update: 2024-06-03