[1]汪 莉,钟素兰,朗么磋,等.促红细胞生成素对大鼠牙髓损伤后修复性牙本质形成的促进作用研究[J].中国美容医学,2024,(7):65-70.
 WANG Li,ZHONG Sulan,LANG Mocuo,et al.Study on Promoting Effect of Erythropoietin on Restorative Dentin Formation after Dental Pulp Injury in Rats[J].Medical Aesthetics and Beauty,2024,(7):65-70.
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促红细胞生成素对大鼠牙髓损伤后修复性牙本质形成的促进作用研究()
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2024年7期
页码:
65-70
栏目:
出版日期:
2024-07-01

文章信息/Info

Title:
Study on Promoting Effect of Erythropoietin on Restorative Dentin Formation after Dental Pulp Injury in Rats
文章编号:
1008-6455(2024)07-0065-06
作者:
汪 莉1 钟素兰2 朗么磋1 张义林1
(1.成都市双流区第一人民医院口腔科 四川 成都 610200 ;2.广州南方医科大学口腔医院口腔科 广东 广州 510280 )
Author(s):
WANG Li1 ZHONG Sulan2 LANG Mocuo1 ZHANG Yilin1
( 1. Department of Stomatology, the First People’s Hospital of Shuangliu District, Chengdu 610200, Sichuan, China; 2. Department of Stomatology, Stomatological Hospital of Southern Medical University, Guangzhou 510280, Guangdong, China )
关键词:
促红细胞生成素牙髓损伤修复修复性牙本质形成牙本质基质蛋白-1牙本质涎磷蛋白
Keywords:
erythropoietin repair of dental pulp injury reparative dentin formation dentin matrix protein-1 dentin sialophosphoprotein
分类号:
R781
文献标志码:
A
摘要:
目的:探究促红细胞生成素(Erythropoietin,EPO)对大鼠牙髓损伤后修复性牙本质形成的作用。方法:分离并 鉴定人牙髓细胞(Dental pulp cells,DPCs),将DPCs分为对照(control)组、肿瘤坏死因子-α(Tumor necrosis factor-α,TNF-α)组、TNF-α+EPO组;使用CCK-8法、Transwell法、碱性磷酸酶染色和茜素红染色法检测细胞增殖、 迁移、成骨分化能力。将30只大鼠随机分为磷酸缓冲盐溶液(Phosphate buffered saline,PBS)组、氢氧化钙[Ca(OH)2] 组和EPO组,对大鼠上颌第一磨牙进行牙髓暴露,分别以含PBS、Ca(OH)2和EPO的胶原蛋白海绵盖髓;术后1个月处死 大鼠,micro-CT和苏木精-伊红染色观察修复性牙本质形成;免疫组化染色和Western blot检测牙本质基质蛋白-1(Dentin matrix protein-1,DMP-1)、牙本质涎磷蛋白(Dentin sialophosphoprotein,DSPP)、Runt相关转录因子2(Runt related transcription factor 2,Runx2)、Osterix(Osx)水平。结果:与control组相比,TNF-α组DPCs增殖、迁移、成骨分化能 力降低(P<0.05);与TNF-α组相比,TNF-α+EPO组DPCs增殖、迁移、成骨分化能力提高(P<0.05)。与PBS组、Ca(OH)2 组相比,EPO组露髓处形成连续完整的钙化桥及大量修复性牙本质,DMP-1、DSPP、Runx2、Osx水平升高(P<0.05)。结论: EPO 通过提高DMP-1、DSPP、Runx2、Osx水平促进牙髓损伤大鼠修复性牙本质形成。
Abstract:
Objective To explore the effect of erythropoietin (EPO) on reparative dentin formation in rats after dental pulp injury. Methods Human dental pulp cells (DPCs) was isolated and identifi ed, and DPCs was divided into control group, tumor necrosis factor-α (TNF-α) group, TNF-α+EPO group. CCK-8 method, Transwell method, ALP staining, and alizarin red staining were used to detect cell proliferation, migration, and osteogenic diff erentiation. 30 rats were randomly divided into PBS group, Ca(OH)2 group, and EPO group. The pulp of the maxillary fi rst molar of the rats was exposed and covered with collagen sponge containing PBS, Ca(OH)2, and EPO respectively. The rats were killed 1 month after surgery, Micro-CT and HE staining was used to observe restorative dentin formation. Immunohistochemical staining and Western blot were used to detect dentin matrix protein-1 (DMP-1), dentin sialophosphoprotein (DSPP), Runt related transcription factor 2 (Runx2) and Osx levels. Results Compared with control group, the proliferation, migration, and osteogenic diff erentiation abilities of DPCs in TNF-α group were decreased (P <0.05). Compared with TNF-α group, the proliferation, migration, and osteogenic diff erentiation abilities of DPCs in TNF-α+EPO group were increased (P <0.05). Compared with PBS group and Ca(OH)2 group, continuous and complete calcifi ed bridges and a large amount of restorative dentin were formed at the pulp exposure point in EPO group, and DMP-1, DSPP, Runx2, and Osx levels were increased (P <0.05). Conclusion EPO promotes reparative dentin formation in rats with pulp injury by increasing DMP-1, DSPP, Runx2, and Osx levels.

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更新日期/Last Update: 2024-07-05