[1]史 敏,李 娜,郭晓波,等.miR-21调控PTEN/PI3K/Akt通路抑制增生性瘢痕形成的机制研究[J].中国美容医学,2019,(03):92-95.
　SHI Min,LI Na,GUO Xiao-bo,et al. Mechanism of miR-21 Inhibiting Human Hypertrophic Scar Formation by RegulatingPTEN/PI3K/Akt Pathway[J].Medical Aesthetics and Beauty,2019,(03):92-95.
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miR-21调控PTEN/PI3K/Akt通路抑制增生性瘢痕形成的机制研究
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2019年03期
页码:
92-95
栏目:
出版日期:
2019-03-11

文章信息/Info

Title:
 Mechanism of miR-21 Inhibiting Human Hypertrophic Scar Formation by Regulating
PTEN/PI3K/Akt Pathway
文章编号:
1008-6455（2019）03-0092-03
作者:
史 敏1; 李 娜2; 郭晓波3; 魏超鹏1; 刘兆旺1; 封兴华1
Author(s):
SHI Min1; LI Na2; GUO Xiao-bo3; WEI Chao-peng1; LIU Zhao-wang1; FENG Xing-hua1
关键词:
 ; [关键词]miR-21; PTEN; 增生性瘢痕; HSF; 细胞外基质; PI3K/Akt
Keywords:
Key words: miR-21;  PTEN;  hypertrophic scars;  hypertrophic scar fibroblasts;  extracellular matrix;  PI3K/Akt
分类号:
R364.3+3
文献标志码:
A
摘要:
[摘要]目的：探讨miR-21对增生性瘢痕中PTEN表达和成纤维细胞增殖的影响，以期为增生性瘢痕的临床诊断和治疗提供新靶
点。方法：分别用qPCR和Western blot检测增生性瘢痕（HTS）组织和细胞中miR-21及PTEN的表达；双荧光素酶报告基因检
测验证miR-21与PTEN的调控关系；Western blot检测miR-21对增生性瘢痕成纤维细胞（HSF）中PTEN蛋白表达影响；MTT检
测细胞增殖能力变化。结果：HTS组织中miR-21高表达，PTEN低表达；双荧光素酶报告实验和Western blot证实了miR-21对
PTEN的靶向调控作用；下调miR-21可增加PTEN的表达，从而可抑制PI3K/Akt通路活性，抑制HSF细胞增殖。结论：本研究证
实了miR-21具有诊断和治疗HTS的潜能，并为临床提供可能的新靶点。

Abstract:
Abstract: Objective To investigate the effect of miR-21 on PTEN expression and fibroblast proliferation in hypertrophic scar,
in order to provide a new target for clinical diagnosis and treatment of hypertrophic scar. Methods The expression of miR-21
and PTEN in HTS tissues and cells were detected by qPCR and western blot, respectively. Dual luciferase reporter gene assay
to verify the regulatory relationship between miR-21 and PTEN. Western blot analysis of the effect of miR-21 on the expression
of PTEN protein in HSF cells. MTT assay for cell proliferation. Results High expression of miR-21 and low expression of
PTEN in HTS tissues. Dual luciferase reporter assay and western blot confirmed the targeted regulation of miR-21 on PTEN.
Down-regulation of miR-21 inhibits PI3K/Akt pathway activity and up-regulates HSF cell proliferation by up-regulating PTEN
expression. Conclusion This study demonstrates the potential of miR-21 to diagnose and treat HTS and may provide new
targets for clinical use.

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更新日期/Last Update: 2019-04-01