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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:

期数:

2022年3期

页码:

87-90

栏目:

出版日期:

2022-03-10

文章信息/Info

Title:

Effects of 0.1% Nano-silver Solution on the Expression of RORT, IL-17 and Foxp3 in Rats with Chronic Periapical Inflammation

文章编号:

1008-6455（2022）03-0087-03

作者:

孙新新¹; 迪丽努尔·阿卜拉¹; 刘 涛²; 罗甜甜¹; 王邦耀¹; 陈 越¹; 刘奕杉¹

[1.新疆医科大学第一附属医院(附属口腔医院)

Author(s):

SUN Xinxin1; Dilinuer·ABULA1; LIU Tao2; LUO Tiantian1; WANG Bangyao1; CHEN Yue1; LIU Yishan1

[1.Department of Pediatric Stomatology-Oral Prophylaxis,the First Affiliated Hospital of Xinjiang Medical University(Affiliated Stomatological Hospital),Xinjiang Uygur Autonomous Region Institute of Stomatology,Urumqi 830000,China; 2.Medical Laboratory Center of the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830000,Xinjiang,China]

关键词:

0.1%纳米银溶液; 慢性根尖周炎; RORγT; IL-17; Foxp3

Keywords:

0.1% nano-silver solution;  chronic periapical disease;  RORT;  IL-17;  Foxp3

分类号:

R781.34

文献标志码:

A [

摘要:

目的：探讨0.1%纳米银溶液用于根管封药对慢性根尖周炎大鼠RORγT、IL-17、Foxp3表达的影响。方法：对40只雄性大鼠双侧下颌第一磨牙开髓建立根尖周炎模型，术后2周处死4只大鼠用RVG+HE判断造模成功，将剩余36只大鼠随机均分为空白对照组（K组），氢氧化钙组（Q组）及0.1%纳米银溶液组（N组）。于用药后7、14、21d各组随机处死4只大鼠，免疫组织化学检测Foxp3表达，qRT-PCR检测RORγT、IL-17的表达。结果：免疫组化显示3组于用药7、14、21d时Foxp3表达呈逐渐上升趋势，N、Q组均高于K组（P＜0.05）。qRT-PCR显示，N组RORγT、IL-17表达在14、21d均低于K组（P＜0.05），21d时，N较Q组IL-17表达无差异（P＞0.05），RORγT表达有差异（P＜0.05）。结论：0.1%纳米银溶液用于根管封药效果好，可降低RORγT、IL-17水平，促进Foxp3的表达。

Abstract:

Objective To investigate the effect of 0.1% nano-silver solution as root canal sealing agent on the expression levels of ROR γT, IL-17 and Foxp3 in rats with chronic periapical inflammation. Methods The model of periapical inflammation was established by opening the pulp of the first mandibular molars of rats. Two weeks after surgery, Hematoxylin-eosin (HE) stainin g was performed to judge the success of modeling. The rats were randomly divided into blank control group (K group), calcium hydroxide paste group (Q group) and 0.1% nano silver solution group (N group). Four rats in each group were randomly sacrificed on day 7, 14 and 21 after administration, and Foxp3 expression was detected by immunohistochemistry. Quantitative real-time polymerase chain reaction (QRT-PCR) was used to detect the expression of RORγT and IL-17. Results Immunohistochemistry showed that Foxp3 expression in group N was significantly higher than that in group K (P＜0.05), and increased gradually with the extension of medication time. QRT-PCR showed that the expression of RORγT and IL-17 in N group was lower than that in K group at 7, 14 and 21?days (P＜0.05), and decreased gradually with time. Conclusion 0.1% nano silver solution was effective for root canal sealing, which could reduce RORγT and IL-17 levels and promote Foxp3 expression

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更新日期/Last Update: 2022-04-07