胎鼠真皮间充质干细胞对小鼠巨噬细胞极化的影响-《中国美容医学》

文章信息/Info

Title:: Effect of Fetal Dermal Mesenchymal Stem Cells on Mouse Macrophage Polarization

作者:: 夏震宇¹ ; 龚思宇²; 王晓川²; 姜笃银¹; 3; 4; 单菲⁴ ; 赵洁⁴; (1.山东大学齐鲁医学院山东济南 250012 ;2.山东省济宁医学院基础医学院山东济宁 272067 ;3.山东大学第二医院整形烧伤科山东济南 250033 ;4.山东大学第二医院急诊医学中心山东济南 250033 )

Author(s):: XIA Zhenyu¹ ; GONG Siyu² ; WANG Xiaochuan³ ; JIANG Duyin¹; 3; 4; SHAN Fei⁴ ; ZHAO Jie⁴; (1.Cheeloo College of Medicine,Shandong University,Jinan 250012,School of Basic Medicine,Jining Medical College,Jining 272067,Shandong,China; 3.Department of Burns and Plastic Surgery,the Second Hospital of Shandong University,Jinan 250033,Shandong,China; 4.Department of Emergency,the Second Hospital of Shandong University,Jinan 250033,Shandong,China)

Keywords:: fetal mice; dermal mesenchymal stem cells; macrophage polarization; M1/M2 macrophage wound healing; coculture

摘要:: 目的：探究胎鼠真皮间充质干细胞（Fetal mice dermal mesenchymal stem cells, FDMSCs）对M1/2型巨噬细胞极化的影响。方法：本研究提取FDMSCs，利用脂多糖（Lipopolysaccharide，LPS）、γ干扰素（Interferon-γ，IFN-γ）刺激RAW 264.7诱导为M1型巨噬细胞，利用白细胞介素4（Interleukin-4，IL-4）刺激RAW264.7诱导为M2型巨噬细胞，并建立了FDMSCs-RAW 264.7共培养体系。共培养24 h后，收集巨噬细胞，用实时定量PCR和流式细胞学检测M1/2型巨噬细胞特征性因子白细胞介素6（Interleukin-6，IL-6）、诱导性一氧化氮合酶（Inducible nitric oxide synthase，iNOS）、CD86、白细胞介素10（Interleukin-10，IL-10）、精氨酸酶1（Arg-1）、CD206的表达变化。结果：FDMSCs使M1型巨噬细胞表达的IL-6、iNOS、CD86减少，IL-10、CD206增多；使M2型巨噬细胞表达的IL-10、Arg-1、CD206表达增多，iNOS表达减少。结论：FDMSCs可以通过调控巨噬细胞极化来调控伤口愈合，对于探索FDMSCs在创面治疗过程中的免疫调节机制有积极意义。

Abstract:: Objective To investigate the eff ect of FDMSCs on the polarisation of M1/2 type macrophages. Methods In this study, RAW264.7 was induced into M1-type macrophages by stimulation with lipopolysaccharide (LPS) and gamma interferon (IFN-γ) and into M2-type macrophages by stimulation with interleukin 4 (IL-4), and a FDMSCs-RAW264.7 co-culture system was established. After 24 hours of co-culture, macrophages were harvested and changes in the expression of the characteristic factors interleukin 6 (IL-6), inducible nitric oxide synthase ( iNOS), CD86, interleukin 10 (IL-10), arginase 1 (Arg-1) and CD206 in M1/2 type macrophages were detected by qRT-PCR and fl ow cytometry. Results FDMSCs decreased the expression of IL-6, iNOS and CD86 and increased the expression of IL-10 and CD206 in M1 macrophages; increased the expression of IL-10, Arg-1 and CD206 and decreased the expression of iNOS in M2 macrophages. Conclusion FDMSCs can regulate wound healing by modulating macrophage polarisation, which has positive implications for exploring the immunomodulatory mechanisms of FDMSCs in the process of wound treatment.