丹参酮ⅡA与隐丹参酮混合物基于TGF-β1抑制人皮肤成纤维细胞的研究-《中国美容医学》

文章信息/Info

Title:: Inhibition of Tanshinone A and Cryptotanshinone Mixture on Human Skin Fibroblasts Based on TGF- 1

Author(s):: XIN Linlin; WANG Haonan; ZHANG Zhe; BAI Yanhong; LIU Hongyan; ZHOU Ying; LI Qingjun; LIU Qian; ZHANG Yongqing; (College of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,Shandong,China)

Keywords:: tanshinone A; cryptotanshinone; human skin fi broblast; transforming growth factor-1; scar

摘要:: 目的：研究丹参酮ⅡA与隐丹参酮混合物对转化生长因子-β1（Transforming growth factor-β1，TGF-β1）诱导人皮肤成纤维细胞（Human skin fibroblasts，HSF）抑制瘢痕形成的影响及分子机制。方法：配制丹参酮ⅡA与隐丹参酮混合物（丹参酮ⅡA与隐丹参酮标准品按照1∶1的比例配置）溶液，采用TGF-β1诱导HSF细胞增殖构建增生性瘢痕体外细胞模型；采用MTT法测定丹参酮ⅡA与隐丹参酮对HSF细胞增殖的影响；采用Western Blot法检测HSF细胞中TGF-β1/Smad信号通路下游信号分子p-Smad2、Smad3蛋白以及Survivin蛋白表达水平变化。结果：丹参酮ⅡA与隐丹参酮能剂量依赖地抑制HSF细胞的增殖，降低p-Smad2、Smad3蛋白和Survivin蛋白表达水平。结论：丹参酮ⅡA与隐丹参酮混合物可能通过下调p-Smad2、3 和Survivin蛋白的水平，抑制HSF细胞的增殖，发挥抑制瘢痕的作用。

Abstract:: Objective To explore the eff ect and mechanism of tanshinone ⅡA and cryptotanshinone mixture on the proliferation of fi broblasts stimulated by transforming growth factor-β1 (TGF-β1), and to provide a theoretical basis for further development and utilization of Danshen. Methods Tanshinone ⅡA and cryptotanshinone mixture ( Tanshinone ⅡA and cryptotanshinone were mixed at a ratio of 1 ∶1) was prepared to treat human skin fi broblasts (HSF) cells which was stimulated by TGF- β1. The eff ect of tanshinone ⅡA and cryptotanshinone on the proliferation of HSF cells was determined using MTT method. The expression levels of protein p-Smad2, 3 and Survivin, downstream signals of TGF-β1/Smad pathway, were determined by western blot (WB). Results The results showed that tanshinone ⅡA and cryptotanshinone could inhibit the proliferation of HSF cells and suppress the expression levels of p-Smad2, 3 and Survivin in a dose-dependent manner. Conclusion Tanshinone ⅡA and cryptotanshinone mixture could inhibit the proliferation of HSF cells by signifi cantly reducing the phosphorylation of Smad2 and 3, and down-regulating the expression level of Survivin.