[1]李 凡,曾 黎,刘朝东,等.VSD联合羟基积雪草苷促进慢性创面愈合和血管生成的机制研究[J].中国美容医学,2023,(9):48-53.[doi:10.15909/j.cnki.cn61-1347/r.005874]
　LI Fan,ZENG Li,LIU Chaodong,et al.Mechanism of VSD Combined with Madecassoside in Promoting Chronic Wound Healing and Angiogenesis[J].Medical Aesthetics and Beauty,2023,(9):48-53.[doi:10.15909/j.cnki.cn61-1347/r.005874]
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VSD联合羟基积雪草苷促进慢性创面愈合和血管生成的机制研究()
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2023年9期
页码:
48-53
栏目:
出版日期:
2023-09-10

文章信息/Info

Title:
Mechanism of VSD Combined with Madecassoside in Promoting Chronic Wound Healing and Angiogenesis
文章编号:
1008-6455（2023）09-0048-06
作者:
李 凡; 曾 黎; 刘朝东; 唐 悦
(四川大学华西广安医院整形美容烧伤修复外科 四川 广安 638000 )
Author(s):
LI Fan; ZENG Li; LIU Chaodong; TANG Yue
(Department of Plastic Surgery and Burn Repair Surgery,West China Guang’an Hospital,Sichuan University,Guangan 638000,Sichuan,China)
关键词:
负压封闭引流; 羟基积雪草苷; 核因子E2相关因子2; 慢性创面; 愈合; 血管生成
Keywords:
vacuum sealing drainage madecassoside Nuclear factor erythroid-2 related factor 2 chronic wounds healing angiogenesis
分类号:
R751.05
DOI:
10.15909/j.cnki.cn61-1347/r.005874
文献标志码:
A
摘要:
目的：探究负压封闭引流（Vacuum sealing drainage，VSD）联合羟基积雪草苷促进慢性创面修复愈合和血管生成 的作用及机制。方法：以不同剂量羟基积雪草苷处理慢性创面模型大鼠进行预实验，通过检测创面愈合时间筛选羟基积雪 草苷最佳作用剂量。将SD大鼠随机分为对照组、模型组、羟基积雪草苷组、VSD组、羟基积雪草苷+VSD组、ML385组、羟 基积雪草苷+VSD+ML385组，每组10只，除对照组外其余各组大鼠构建慢性创面模型，以药物分组处理后，检测各组大鼠 创面愈合率及微循环血流灌注值（Microcirculation perfusion value，MPD）；免疫组织化学染色检测各组大鼠创面微 血管密度（Microvessel density ，MVD）；酶标仪检测各组大鼠血清内皮细胞生长因子（Vascular endothelial growth factor，VEGF）、促血管生成素1（Angiopoietin 1，Ang1）及创面组织活性氧（Reactive Oxygen Species，ROS）、丙二 醛（Malondialdehyde，MDA）、环氧化酶-2（Cyclooxygenase 2，COX-2）、白细胞介素-6（Interleukin-6，IL-6）水平； 免疫印迹检测各组大鼠创面组织VEGF、Ang1与Nrf2/血红素加氧酶-1（Heme oxygenase-1，HO-1）通路蛋白表达。结果：与 对照组相比，模型组大鼠创面MPD及MVD、血清VEGF及Ang1、创面组织VEGF及Ang1、Nrf2、HO-1蛋白表达降低（P＜0.05）， 创面组织ROS、MDA、COX-2、IL-6水平明显升高（P＜0.05）。与模型组相比，羟基积雪草苷组、VSD组、羟基积雪草苷+VSD组 大鼠创面愈合率、MPD及MVD、血清VEGF及Ang1、创面组织VEGF及Ang1、Nrf2、HO-1蛋白表达升高（P＜0.05），创面组织ROS、 MDA 、COX-2、IL-6水平降低（P＜0.05），且羟基积雪草苷和VSD联合应用作用比单独应用更强；ML385组各指标变化与羟基 积雪草苷+VSD组相反，且ML385可逆转羟基积雪草苷和VSD联合应用对模型大鼠各指标变化的作用。结论：VSD和羟基积雪草 苷两者联合可协同上调Nrf2/HO-1信号通路蛋白表达，进而促进慢性创面修复愈合和血管生成。
Abstract:
Objective To explore the eff ects and mechanism of vacuum sealing drainage (VSD) combined with madecassoside in promoting slow wound healing and angiogenesis. Methods Treatment of chronic wound model rats with diff erent doses of madecassoside and screening of the optimal dosage of madecassoside by detecting wound healing time. The rats in each group were constructed with chronic wounds models, and after grouping with drugs, the wound healing time of the rats in each group was detected. SD rats were randomly grouped into control group, model group, madecassoside group, VSD group, madecassoside+VSD group, ML385 group, and madecassoside+VSD+ML385 group, 10 rats in each group. Except for the control group, the rats in the other groups were constructed with chronic wound models, and after grouping with drugs, the wound healing rate and the microcirculation perfusion value (MPD) of the rats in each group were detected, immunohistochemical staining was used to detect wound microvessel density (MVD) of rats in each group, the microplate reader was used to detect the levels of endothelial cell growth factor (VEGF), angiopoietin 1 (Ang1) in serum, and reactive oxygen species (ROS), malondialdehyde (MDA), cyclooxygenase-2 (COX-2), and interleukin-6 (IL-6) in wound tissue in each group, and Western blot was used to detect the expressions of VEGF, Ang1 and Nrf2/HO-1 pathway proteins in wound tissue of rats in each group. Results Compared with the control group, the expressions of MPD and MVD in the wound tissue, the levels of VEGF and Ang1 in serum, the protein expressions of VEGF and Ang1, Nrf2, and HO-1 in the wound tissue of the rats in the model group were decreased (P＜0.05), the levels of ROS, MDA, COX-2 and IL-6 in the wound tissue were obviously increased (P＜0.05). Compared with the model group, the wound healing rate, MPD and MVD, the levels of VEGF and Ang1 in serum, the protein expressions of VEGF and Ang1, Nrf2, and HO-1 in the wound tissue in the madecassoside group, VSD group, and madecassoside+VSD group were obviously increased (P ＜0.05), and the eff ect of combined application of hydroxyasiaticoside and VSD was stronger than that of single application, The change of each index in ML385 group was opposite to that in hydroxyasiaticoside + VSD group, and ML385 could reverse the eff ect of hydroxyasiaticoside and VSD combined application on the change of each index in model rats. Conclusion The combination of VSD and madecassoside can synergistically up-regulate the expression of Nrf2/HO-1 signaling pathway protein, thereby promoting the repair and healing of chronic wounds and angiogenesis.

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更新日期/Last Update: 2023-09-22