[1]倪 娜,周炳荣,马委委,等. miRNA-769-5p上调对正常人成纤维细胞生物活性的影响[J].中国美容医学,2018,(04):109-111.
　NI Na,ZHOU Bing-rong,MA Wei-wei,et al. Effect of miRNA-769-5p on Biological Activity of Normal Human Fibroblasts[J].Medical Aesthetics and Beauty,2018,(04):109-111.
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 miRNA-769-5p上调对正常人成纤维细胞生物活性的影响
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2018年04期
页码:
109-111
栏目:
出版日期:
2018-05-10

文章信息/Info

Title:
 Effect of miRNA-769-5p on Biological Activity of Normal Human Fibroblasts
文章编号:
8-6455（2018）04-0109-03
作者:
 倪 娜; 周炳荣; 马委委; 骆 丹
Author(s):
 NI Na; ZHOU Bing-rong; MA Wei-wei; LUO Dan
关键词:
 ; [关键词]miRNA; 成纤维细胞; 紫外线; 光损伤
Keywords:
 ; Key words: miRNA;  fibroblasts;  ultraviolet;  photodamage
分类号:
R329.2+8
文献标志码:
A
摘要:

（南京医科大学第一附属医院皮肤科 江苏 南京 210029）
[摘要]目的：探讨上调miRNA-769-5p表达对正常人成纤维细胞生物活性的影响。方法：将miRNA-769-5p mimics转染至
人成纤维细胞中，CCK-8试剂盒测定细胞增殖率，荧光显微镜下观察氧化损伤荧光，流式细胞仪测定荧光值与细胞凋亡
率，将通过转染miRNA-769-5p mimics上调miRNA-769-5p表达的转染组与未经处理的对照组成纤维细胞进行比较。结果：
转染组miRNA-769-5p相对表达量为（1.36±0.2）显著高于对照组的（1.0±0.1），差异有统计学意义（P ＜0.05）；转
染组细胞增值率为（0.747±0.072）明显低于对照组的（1.007±0.025），差异有统计学意义（P ＜0.05）；转染组细胞
荧光值（92.067±3.092）明显高于对照组的（21.600±8.487），差异有统计学意义（P ＜0.05）；转染组细胞凋亡率为
（49.299±5.184）%明显高于对照组的（5.977±0.939）%，差异有统计学意义（P ＜0.05）。结论：上调miRNA-769-5p可诱
导出类似紫外线光损伤效应，对正常人成纤维细胞生物活性产生影响。

Abstract:

Abstract: Objective To investigate the effect of miRNA-769-5p on biological activity in normal human fibroblasts. Methods
Human fibroblasts were transfected with miRNA-769-5p mimic. CCK-8 kit was performed to test proliferation rate. Cell were
observed under fluorescence microscope. Flow cytometer was used to measured rate of reactive oxygen species and apoptosis.
All the parameters of the mimics group were compared with the control group. Results The relative expression of miRNA-
769-5p in the mimics group was (1.36±0.2), which was significantly higher than that of the control group (1.0±0.1), the
difference was statistically significant(P ＜0.05). The cell proliferation rate in the mimics group (0.747±0.072) was significantly
lower than that of the control group (1.007±0.025)(P ＜0.05). The fluorescence value of the mimics group (92.067±3.092)
was significantly higher than that of the control group (21.600±8.487)(P ＜0.05). The apoptotic rate in the mimics group was
(49.299 ± 5.184)%, which was significantly higher than that of the control group (5.977±0.939)%(P ＜0.05). Conclusion
Up-regulation of miRNA-769-5p induce photo-damage and have effect on biological activity in normal human fibroblasts.

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更新日期/Last Update: 2018-06-19