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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:

期数:

2019年03期

页码:

100-103

栏目:

出版日期:

2019-03-11

文章信息/Info

Title:

 Study on the Apoptosis of Skin Fibroblasts Induced by UVB in Saussurea
Medusa Maxim Polysaccharides

文章编号:

1008-6455（2019）03-0100-04

作者:

孙 楠¹; 吴 艳²; 姜 莉¹

Author(s):

SUN Nan1; WU Yan2; JIANG Li1

关键词:

[关键词]水母雪莲多糖; 中波紫外线; 成纤维细胞; 皮肤; 诱导; 凋亡

Keywords:

 ; Key words: Saussurea medusa Maxim polysaccharides;  medium wave ultraviolet;  fibroblast;  skin;  induction;  apoptosis

分类号:

R329.2+8

文献标志码:

A

摘要:

[摘要]目的：研究水母雪莲多糖对中波紫外线刺激下人皮肤成纤维细胞凋亡的影响及可能的机制。方法：利用蒸馏水回流提
取+醇沉分离法提取水母雪莲中的多糖成分；使用包皮切除术后皮肤，处理后在6孔板中定量接种经10%胎牛血清DMEM培养基
传代培养的细胞。细胞体外培养并分为空白对照组、紫外线照射组、10mg/ml水母雪莲多糖干预组和40mg/ml水母雪莲多糖干
预组。除空白对照组外，其他组细胞分别在低辐射UVB（L-UVB，30mJ/cm2辐射1h）和高辐射UVB（H-UVB，60mJ/cm2辐射1h）
两种条件下接受紫外线照射。在暴露于UVB光前，10mg/ml水母雪莲多糖干预组和40mg/ml水母雪莲多糖干预组细胞以终浓
度为10mg/ml和40mg/ml水母雪莲多糖培养1h，紫外线组不干预。照射1h后，将细胞在37℃，10%胎牛血清DMEM培养基中温育
18h。测定SOD、GSH、CAT及MDA水平，流式细胞术检测细胞凋亡，Western blot测定p38、p53、Bcl-2、Bax蛋白及caspase-3
的β肌动蛋白含量变化。结果：水母雪莲多糖可以提高经中波紫外线损伤的成纤维细胞中SOD、CAT的活性和GSH含量，并可
减少MDA含量，通过上调p38，p53和Bcl-2的表达，同时下调Bax和活性caspase-3的表达。与非干预组相比，无论暴露于低剂
量还是高剂量辐射下，10mg/ml和40mg/ml水母雪莲多糖组的成纤维细胞凋亡数目都要更少。结论：水母雪莲多糖可以通过上
调人成纤维细胞p38，p53和Bcl-2的表达、下调Bax和活性caspase-3的表达、增加SOD、CAT的活性和GSH的含量、减少MDA含
量,从而抑制中波紫外线照射所致的细胞凋亡。

Abstract:

Abstract: Objective To investigate the effect of Saussurea medusa Maxim polysaccharides on apoptosis of human fibroblasts
exposed to UVB and to study possible mechanisms. Methods Distilled water was used for reflux extraction and alcohol
precipitation filtration method was used to extract Saussurea medusa Maxim polysaccharides. After skin was removed by
foreskin, treated with 10% fetal bovine serum DMEM medium and inoculated in 96-well plates and 6-well plates for later
use. Cells were cultured in vitro and divided into blank control group, ultraviolet irradiation group, 10mg/ml jellyfish jellyfish
intervention group, and 40mg/ml jellyfish polysaccharide intervention group. Each group of cells was exposed to ultraviolet
light under two conditions of low-irradiation UVB (L-UVB, 30mJ/cm2 radiation 1h) and high-radiation UVB (H-UVB,60mJ/
cm2 radiation 1h). Before exposed to UVB light, cells of the experimental group were cultured for 1 hour at a final concentration
of 10mg/ml and 40mg/ml Saussurea medusa Maxim polysaccharides. The UV group did not interfere, and the blank control
group did not receive irradiation. After 1 hour of irradiation, cells were incubated in 10% fetal bovine serum DMEM medium
for 18 hours at 37℃. The levels of SOD, GSH, CAT and MDA were measured. Apoptosis was detected by flow cytometry. The
changes of β-actin in p38, p53, Bcl-2, Bax and caspase-3 were determined by western blot. Results The Saussurea medusa
Maxim polysaccharides can increase the activity of SOD, CAT and the content of GSH in fibroblasts damaged by UVB, and
decrease the content of MDA. The expression of p38, p53 and Bcl-2 was up-regulated, and the expression of Bax and active
caspase-3 was down-regulated at the same time. No matter when exposed to low or high doses of radiation, the number of
fibroblasts apoptosis in 10mg/ml and 40mg/ml Saussurea medusa Maxim polysaccharides group was lower than that in the
control group. Conclusion Saussurea involucrata can up-regulate p38, p53 and Bcl-2, down-regulate the expression of Bax and
active caspase-3, and inhibit the expression of MDA by increasing SOD activity, GSH content, CAT activity and reducing MDA
content. Irradiation leads to apoptosis of fibroblasts.

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更新日期/Last Update: 2019-04-01