角质形成细胞生长因子促进大鼠皮肤伤口局部血管化效果研究<br />-《中国美容医学》

文章信息/Info

Title:: Keratinocyte Growth Factor Promotes Vascularization of Rat Skin Wounds

Author(s):: ZHANG Kai; CAO Cong; YU Meng-liu; GUANG Meng-kai; ZHONG Bo; XU Bao-hua

Keywords:: Key words: keratinocyte growth factor(KGF); skin wound; wound healing; collagen membrane; angiogenesis

摘要:: [摘要]目的：探索角质形成细胞生长因子（Keratinocyte growth factor，KGF）对于大鼠皮肤伤口局部的促血管化作用。
以探究其可能的促进愈合的机理。方法：构建大鼠背部皮肤全层缺损模型，随机分为空白组（不作特殊处理），空白膜组
（放置空白胶原膜），KGF膜组（放置加载KGF的胶原膜）。通过大体标本观察，组织学染色、免疫双标染色等技术对术后7d
和14d时伤口的血管化程度进行比较和分析。结果：术后通过大体标本观察，可看到空白组和空白膜组虽均有血管长入伤口
内，但中心部位血管化程度低于KGF膜组。Masson染色和免疫荧光染色及局部血管计数也证实加载KGF的胶原膜可以促进局部
血管再生。结论：KGF可以促进大鼠皮肤伤口愈合过程中的血管化作用。

Abstract:: Abstract: Objective This article aims to explore the pro-angiogenic effect of KGF on rat skin wounds. Methods After
successful modeling of full-thickness defect, 12 rats were randomly divided into three groups. The blank group was not treated.
Blank collagen membrane was placed in the blank membrane group. Collagen membrane loaded with KGF was placed in the
KGF membrane group. The degree of vascularization of the wounds at 7 and 14 days after surgery was compared and analyzed.
Analysis was performed by gross specimen observation of wounds, histological staining, and immunolabeling. Results
Through the observation of gross specimens, it can be seen that although wounds in the blank group and the blank membrane
group have blood vessels that grow into the wound, the degree of vascularization in the central part was lower than that in
the KGF membrane group. Masson staining and immunofluorescence staining also confirmed that collagen membranes with
sustained release of KGF promoted local angiogenesis. Conclusion KGF can promote the vascularization of rat skin wound
healing process.