MAPKs信号通路在内皮素-1诱导的牙周膜干细胞成骨分化过程中的作<br />用研究<br />-《中国美容医学》

文章信息/Info

Title:: Effects of MAPKs Signaling Pathway in Osteogenic Differentiation of Periodontal
Ligament Stem Cells Induced by Endothelin-1

Keywords:: Key words: periodontal ligament stem cells; endothelin-1(ET-1); mitogen-activated protein kinases; Runx2; OCN; periodontitis

摘要:: [ 摘要] 目的：研究丝裂原激活蛋白激酶( M i t o g e n - a c t i v a t e d p r o t e i n k i n a s e s ， M A P K s ) 信号通路在内皮
素-1(Endothelin-1，ET-1)诱导的牙周膜干细胞（Periodontal ligament stem cells，PDLSCs）成骨分化中的调控机制。
方法：体外培养牙周膜干细胞，于ET-1处理前分别加入ERK通路抑制剂PD98059，JNK通路抑制剂SP600125，p38α通路抑制剂
SB203580。以未作任何处理的牙周膜干细胞为空白对照，以单独加入抑制剂的牙周膜干细胞为阴性对照，用100nM ET-1干预
不同组牙周膜干细胞14d，通过实时定量PCR及Western blot技术检测细胞Runx2和OCN基因和蛋白的表达情况。结果：ET-1对
牙周膜干细胞Runx2和OCN基因及蛋白表达具有显著促进作用，但PD98059抑制了ET-1对牙周膜干细胞Runx2和OCN表达的促进
效应，而SP600125和SB203580的抑制作用不显著。结论：ET-1通过ERK信号通路调控牙周膜干细胞Runx2和OCN的表达。

Abstract:: Abstract: Objective To investigate the role of mitogen activated protein kinases (MAPKs) pathway on endothelin-1-
induced osteogenic differentiation of periodontal ligament stem cells (PDLSCs). Methods The PDLSCs were stimulated
with endothelin-1(ET-1), ERK pathway inhibitor PD98059, JNK pathway inhibitor SP600125 and p38α pathway inhibitor
SB203580 for 14 days, respectively. The untreated PDLSCs served as normal control and the PDLSCs with inhibitor alone
served as negative control. The expression of Runx2 and OCN mRNAs and proteins in PDLSCs were detected by real-time
PCR and western blot, respectively. Results ET-1 significantly promoted the expression of Runx2 and OCN mRNAs and
proteins in PDLSCs. PD98059 inhibited the effect of endothelin-1 on the expression of Runx2 and OCN in PDLSCs. However
the inhibition of SP600125 and SB203580 was not significant. Conclusion These results indicate that ET-1 may modulate the
expression of Runx2 and OCN via ERK signaling pathway in PDLSCs.