[1]刘立柱,李超艺,林诗炜,等.小鼠10%皮肤软组织缺损创伤后应激障碍抑郁样行为模型的构建及其发生机制探讨[J].中国美容医学,2022,(5):98-102.
　LIU Lizhu,LI Chaoyi,LIN Shiwei,et al.Construction and Mechanism of Depression-like Behavior Model of Post-traumatic Stress Disorder in Mice with 10% Skin and Soft Tissue Defects[J].Medical Aesthetics and Beauty,2022,(5):98-102.
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小鼠10%皮肤软组织缺损创伤后应激障碍抑郁样行为模型的构建及其发生机制探讨()
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2022年5期
页码:
98-102
栏目:
出版日期:
2022-05-10

文章信息/Info

Title:
Construction and Mechanism of Depression-like Behavior Model of Post-traumatic Stress Disorder in Mice with 10% Skin and Soft Tissue Defects
文章编号:
1008-6455（2022）05-0098-05
作者:
刘立柱; 李超艺; 林诗炜; 曲 野
(海南医学院第二附属医院创伤外科 海南 海口 570311 )
Author(s):
LIU Lizhu; LI Chaoyi; LIN Shiwei; QU Ye
(Department of Traumatology,the Second Affiliated Hospital of Hainan Medical College,Haikou 570311,Hainan,China)
关键词:
皮肤软组织缺损; 创伤后应激障碍; 单胺氧化酶A; 抑郁样行为; 小鼠; 动物模型
分类号:
R749.99
文献标志码:
A
摘要:
目的：探究10%皮肤软组织缺损构建小鼠创伤后应激障碍抑郁样行为模型的可行性，并探讨其发生机制。方法：32只C57BL/6小鼠，雌雄各半，随机分为对照组-雄性，对照组-雌性，创伤后应激障碍-雄性，创伤后应激障碍-雌性，共四组。取创伤后应激障碍组小鼠，背部剃毛，去除背部约10%面积的皮肤软组织，深度达深筋膜层。记录小鼠体重变化，7 d后，取小鼠脑组织用于HE染色，使用ELISA试剂盒检测脑组织中单胺类神经递质5-HT、NE、DA与炎性细胞因子TNF-α、IL-6水平，采用Western blot检测小鼠脑组织中MAOA表达水平。结果：10%皮肤软组织缺损造成小鼠体重短期内迅速下降，与对照组相比，无论雄性还是雌性小鼠，糖水偏好摄取率、穿越平台次数显著减少，悬尾不动时间与强迫游泳不动时间显著增加，差异有统计学意义（P＜0.05）。10%皮肤软组织缺损导致小鼠脑组织海马区神经元损伤，炎性细胞因子TNF-α、IL-6分泌水平增加，单胺类神经递质5-HT、NE、DA浓度下降，差异有统计学意义（P＜0.05）。与对照组相比，无论雄性还是雌性小鼠脑组织中MAOA表达水平显著增加，差异有统计学意义（P＜0.05）。结论：采用10%皮肤软组织缺损构建小鼠创伤后应激障碍抑郁样行为模型是可行的，其机制可能是通过增加MAOA表达，降低单胺类神经递质5-HT、NE、DA表达水平。

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更新日期/Last Update: 2022-05-31