«上一篇/Previous Article|本期目录/Table of Contents|下一篇/Next Article»

《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:

期数:

2024年9期

页码:

45-48

栏目:

出版日期:

2024-08-26

文章信息/Info

Title:

Evaluation of Curative Effect of Self-made Chinese Herbal Oil on Deep Burn Wound in Rats

文章编号:

1008-6455（2024）09-0045-04

作者:

杜 宪1 ; 蔡 霞1 ; 任纪祯1 ; 苏鲁安2 ; 陈振雨1

(1.青岛大学附属医院整形外科 山东 青岛 266000 ;2.青岛君良烧伤医院烧伤外科 山东 青岛 266000 )

Author(s):

DU Xian1 ;  CAI Xia1 ;  REN Jizhen1 ;  SU Lu’an2 ;  CHEN Zhenyu1

( 1.Department of Plastic Surgery, Affi liated Hospital of Qingdao University, Qingdao 266000, Shandong, China; 2.Department of Burn Surgery, Qingdao Junliang Burn Hospital, Qingdao 266000, Shandong, China )

关键词:

自制中草药油; 大鼠; 深度烧伤; 创面愈合; 疗效评价

Keywords:

self-made Chinese herbal oil;  rat;  deep burn;  wound healing;  effi cacy evaluation

分类号:

R285.5

文献标志码:

A

摘要:

目的：经过建立Wister大鼠深度烧伤模型，探讨自制中草药油机制及促进深度烧伤创面愈合的临床疗效。方法：选取 清洁健康雄性Wister大鼠60只为研究对象，将建模成功的大鼠依据治疗方式分为对照组30只和观察组30只。对照组使用生 理盐水予以清创后，将凡士林纱布遮盖包裹创面，并用敷贴稳固；观察组采用自制中草药油涂抹，涂抹均匀后予以纱布进 行固定，每日进行换药1次。对比两组治疗后第3、7、14、21天创面愈合情况与创面愈合率、HE染色法观察大鼠烧伤创面皮 肤成纤维细胞数及毛细血管数，免疫组化染色法检测大鼠烧伤创面皮肤组织表皮生长因子（Epidermal growth factor， EGF ）、血管内皮生长因子（Vascular endothelial growth factor，VEGF）水平。结果：治疗后第3、7、14、21天，两 组创面愈合率均升高，且观察组明显高于对照组（P＜0.05）；观察组创面愈合面积和渗出液均小于对照组；两组创面成 纤维细胞数和毛细血管数均上升，且观察组明显高于对照组（P＜0.05）；两组EGF、VEGF水平均上升，且观察组比对照组 高（P＜0.05）。结论：利用自制中草药油治疗大鼠深度烧伤创面，可有效增加创面成纤维细胞数和毛细血管数，提高创面 EGF 、VEGF水平，促进了创面愈合情况，为临床深度烧伤创面用药提供有力理论价值。

Abstract:

Objective To explore the mechanism of self-made Chinese herbal oil and the clinical effi cacy of promoting deep burn wound healing by establishing a Wister rat model of deep burn. Methods Sixty clean and healthy male Wister rats were selected for the study, and the modeled rats were divided into 30 control rats and 30 observation rats according to the treatment. After debridement with normal saline, the wound of control group was covered with vaseline gauze and fi xed, the observation group was smeared with self-made Chinese herbal oil and fi xed with Vaseline gauze after evenly smearing, the dressing was changed once every other day. The wound healing conditions and wound healing rates of the two groups at the 3rd, 7th, 14th and 21st days after treatment were compared, the number of fi broblasts and capillaries on the burn wound of rats was observed by HE staining method, the epidermal growth factor (EGF) and vascular endothelial growth factor (VEGF) levels on the burn wound of rats was detected by immunohistochemical staining method. Results On the 3rd, 7th, 14th and 21st day after treatment, the wound healing rate of the two groups was increased, and the observation group was signifi cantly higher than the control group (P ＜0.05), the wound healing area and exudate in the observation group were smaller than those in the control group. The number of fi broblasts and capillaries increased in the two groups, and the observation group was signifi cantly higher than the control group (P ＜0.05), the levels of EGF and VEGF were increased in the two groups, and the observation group was higher than the control group (P＜0.05). Conclusion The use of self-made Chinese herbal oil in the treatment of deep burn wounds in rats can eff ectively increase the number of fi broblasts and capillaries, improve the levels of EGF and VEGF, and promote the healing of wounds.

常用功能

更新日期/Last Update: 2024-08-27