[1] 赵超然,王湘琦,熊爱兵. 低氘水联合富血小板血浆对糖尿病大鼠胰岛细胞的保护作用及TGF-1表达的影响[J].中国美容医学,2021,(6):101-106.
　 ZHAO Chao-ran,WANG Xiang-qi,XIONG Ai-bing. Effects of Deuterium-depleted Water Combined with Platelet-rich Plasma on IsletCell Protection and TGF-β1 Expression in Diabetic Rats[J].Medical Aesthetics and Beauty,2021,(6):101-106.
点击复制

 低氘水联合富血小板血浆对糖尿病大鼠胰岛细胞的保护作用及TGF-β1表
达的影响
()
分享到：

《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2021年6期
页码:
101-106
栏目:
出版日期:
2021-06-10

文章信息/Info

Title:
 Effects of Deuterium-depleted Water Combined with Platelet-rich Plasma on Islet
Cell Protection and TGF-β1 Expression in Diabetic Rats
文章编号:
1008-6455（2021）06-0101-06
作者:
 赵超然; 王湘琦; 熊爱兵
Author(s):
 ZHAO Chao-ran; WANG Xiang-qi; XIONG Ai-bing
关键词:
 [关键词]低氘水; 富血小板血浆; 糖尿病; 胰岛细胞; 溃疡创面; 转化生长因子β1
Keywords:
 Key words: deuterium-depleted water;  platelet-rich plasma(PRP);  diabetes;  islet cells;  ulcer wounds;  transforming growthfactor-β1
分类号:
R587.1
文献标志码:
A
摘要:
[摘要]目的：观察低氘水(Deuterium-depleted water，DDW)联合富血小板血浆(Platelet-rich plasma，PRP)对糖尿病大
鼠胰岛细胞保护作用及TGF-β1表达的影响，初步探讨其对保护糖尿病大鼠胰岛细胞、促进糖尿病溃疡愈合的可能机制。方
法：通过高糖高脂饲料喂养配合腹腔注射链脲菌素（STZ）+背部皮肤全层切除建立糖尿病大鼠溃疡模型。成模后按随机数字
法分为糖尿病模型组、低氘水组（DDW）、富血小板血浆组（PRP）和低氘水联合富血小板血浆组（DDW+PRP）。空白对照组
采用腹腔注射柠檬酸-柠檬酸钠缓冲液+背部皮肤全层切除建模。各组大鼠分别于治疗后3d、7d、14d检测随机血糖，创面愈
合率。胰腺组织HE染色后观察其病理学改变，酶联免疫吸附法检测创面组织TGF-β1表达情况。结果：DDW组、DDW+PRP组干
预14d后随机血糖较干预前降低，差异具有统计学意义（P ＜0.05）。DDW组、PRP组、DDW+PRP组在干预7d、14d后创面愈合率
高于糖尿病模型组，差异具有统计学意义（P ＜0.05）；DDW+PRP组在干预14d时创面愈合率高于DDW组、PRP组，差异具有统
计学意义（P ＜0.05）；且与空白对照组相比，差异无统计学意义（P ＞0.05）。胰岛组织学观察随干预时间延长，DDW组、
DDW+PRP组较前相比，胰岛细胞在形态、数量、排列方式、染色颗粒分布情况均有明显改观。DDW+PRP组干预后7d、14d后
TGF-β1含量高于DDW组、PRP组，差异具有统计学意义（P ＜0.05）；而DDW组、PRP组相比差异无统计学意义（P ＞0.05）。
结论：低氘水联合富血小板血浆对2型糖尿病大鼠溃疡创面的愈合有显著促进作用，且低氘水对2型糖尿病大鼠胰岛细胞具有
一定保护和修复作用。促进创面愈合机制可能与降低糖尿病大鼠随机血糖，改善胰岛细胞功能，提高创面组织中TGF-β1含
量有关。

Abstract:
Abstract: Objective To observe the effect of deuterium-depleted water (DDW) combined with platelet-rich plasma (PRP)
on the islet cell protection and TGF-β1 expression in diabetic rats, and explore its possible mechanism to protect islet cells of
diabetic rats and promote the healing of diabetic ulcer. Methods The ulcer model of diabetic rats were established by feeding
high-sugar and high-fat feed combined with intraperitoneal injection of streptozotocin (STZ)+full-thickness resection of back
skin. After modeling, they were divided into diabetes model group, deuterium-depleted water group (DDW), platelet-rich
plasma group (PRP) and deuterium-depleted water combined with platelet-rich plasma group (DDW+PRP) according to random
numbers. The blank control group was modeled by intraperitoneal injection of citric acid-sodium citrate buffer+full-thickness
resection of the back skin.Rats in each group were tested for random blood glucose and wound healing rate 3d, 7d, 14d after
treatment. The pathological changes were observed after HE staining of pancreatic tissue.Enzyme-linked immunosorbent
assay was used to detect the expression of TGF-β1 in wound tissue. The pathological changes were observed. Enzyme-linked
immunosorbent assay was used to detect the expression of TGF-β1 in wound tissue. Results The random blood glucose in
the DDW group and DDW+PRP group after 14 days of intervention was lower than before, the difference was statistically
significant (P ＜0.05). The wound healing rate of the DDW group, PRP group, and DDW+PRP group was higher than the
diabetes model group after 7d and 14d intervention (P ＜0.05). The wound healing rate of the DDW+PRP group was higher
than DDW group and PRP group (P ＜0.05), and compared with the blank control group, the difference was not statistically
significant (P ＞0.05). With the extension of the intervention time, compared with the previous groups, the islet cells in the
DDW group and DDW+PRP group were significantly improved in their morphology, number, arrangement, and distribution
of stained particles. The content of TGF-β1 in the DDW+PRP group was higher than the DDW group and PRP groups at 7d
and 14d after intervention (P ＜0.05), but there were no significant difference between the DDW and PRP groups (P ＞0.05).
Conclusion Deuterium-depleted water combined with PRP can significantly promote the healing of ulcer wounds in type
2 diabetic rats, and deuterium-depleted water can protect and repair the islet cells of type 2 diabetic rats. The mechanism of
promoting wound healing may be related to reducing the random blood glucose of diabetic rats, improving the function of islet
cells, and increasing the content of TGF-β1 in the wound tissue.

相似文献/References:

[1]陈 健,谢包根,黄永新,等. 富血小板血浆联合清创术治疗难愈性创面的疗效观察[J].中国美容医学,2018,(04):11.
　CHEN Jian,XIE Bao-gen,HUANG Yong-xin,et al. Effect of the Platelet Rich Plasma Combined with Debridement in the Treatment of Refractory Wounds[J].Medical Aesthetics and Beauty,2018,(6):11.
[2]崔晓美,丁晓东,陈晓明,等.微针疗法联合PRP治疗痤疮凹陷性瘢痕疗效分析[J].中国美容医学,2018,(10):27.
　CUI Xiao-mei,DING Xiao-dong,CHEN Xiao-ming,et al.Clinical Application and Experience of Microacupuncture Combined with PRP forTreatment of Acne Depression Scar[J].Medical Aesthetics and Beauty,2018,(6):27.
[3]朱旭冉,刘林嶓,李广帅,等. 颗粒脂肪联合PRP及Nanofat治疗面部凹陷的疗效观察[J].中国美容医学,2018,(12):31.
　ZHU Xu-ran,LIU Lin-bo,LI Guang-shuai,et al. A Preliminary Study on the Effect of Granule Fat Combined with PRP and Nanofat to Reshape the Facial Contour[J].Medical Aesthetics and Beauty,2018,(6):31.
[4]张琼玲,刘 清,余银霞. 毛发移植联合富血小板血浆注射在雄激素性秃发患者治疗中的应用分析[J].中国美容医学,2018,(12):60.
　ZHANG Qiong-ling,LIU Qing,YU Yin-xia.The Value of Hair Transplantation Combined with Platelet-Rich Plasma in the Treatment of Androgen Alopecia[J].Medical Aesthetics and Beauty,2018,(6):60.
[5]李 凯,秦 巍,周 明,等. 自体脂肪颗粒结合富血小板血浆(PRP)移植在面部年轻化治疗中的应用研究[J].中国美容医学,2019,(12):17.
　LI Kai,QIN Wei,ZHOU Ming,et al. Application of Autogenous Fat Granule Combined with Platelet-rich Plasma (PRP)Transplantation in the Treatment of Facial Rejuvenation[J].Medical Aesthetics and Beauty,2019,(6):17.
[6]陈 航,杨景军,魏子人,等. 复合皮移植技术与富血小板血浆对烧伤后大面积瘢痕整复患者血清炎症因子水平及美观度的影响[J].中国美容医学,2020,(2):44.
　CHEN Hang,YANG Jing-jun,WEI Zi-ren,et al. Effect of Composite Skin Transplantation and Platelet-rich Plasma on the Level ofSerum Infl ammatory Factors and Aesthetic Degree in Patients with Large Area Scarafter Burn[J].Medical Aesthetics and Beauty,2020,(6):44.
[7] 徐 旻,吕 君,陈尚周,等. 超脉冲CO2点阵激光联合PRP治疗面部痤疮凹陷性瘢痕疗效分析[J].中国美容医学,2020,(5):41.
　 XU Min,LV Jun,CHEN Shang-zhou,et al. Clinical Analysis of Super Pulse CO2 Lattice Laser Combined with PRP in theTreatment of Atrophic Acne Scar[J].Medical Aesthetics and Beauty,2020,(6):41.
[8] 王凤兰,刘 雷. 自体脂肪结合富血小板血浆注射应用于面部年轻化中的效果分析[J].中国美容医学,2020,(5):86.
　 WANG Feng-lan,LIU Lei. Analysis on Effect of Autologous Fat Combined with Platelet-rich Plasma Injection onFacial Rejuvenation[J].Medical Aesthetics and Beauty,2020,(6):86.
[9] 管晓玉 纪 覃 吴 灿 综述,白 南 审校. C02点阵激光治疗皮肤创伤性瘢痕的临床应用及研究进展[J].中国美容医学,2020,(7):169.
　 GUAN Xiao-yu,JI Tan,WU Can,et al. Clinical Application and Research Progress of CO2 Fractional Laser in the Treatmentof Traumatic Skin Scar:A Systematic Review of Literature[J].Medical Aesthetics and Beauty,2020,(6):169.
[10] 辛增桃,田 朦,靳林昊,等. 纳米脂肪联合PRP注射治疗增生性瘢痕[J].中国美容医学,2020,(10):69.
　 XIN Zeng-tao,TIAN Meng,JIN Lin-Hao,et al. Therapeutic Effect of Nanofat Combined with PRP Injection in the Treatment ofHypertrophic Scar[J].Medical Aesthetics and Beauty,2020,(6):69.

更新日期/Last Update: 2021-07-13