[1] 张博伦,贾 如,胡 波,等. 不同咀嚼负荷对牙周组织炎症状态影响的实验研究[J].中国美容医学,2020,(8):23-27.
　 ZHANG Bo-lun,JIA Ru,HU Bo,et al. Effects of Infl ammation for Periodontitis Tissue on Different Masticatory Loading[J].Medical Aesthetics and Beauty,2020,(8):23-27.
点击复制

 不同咀嚼负荷对牙周组织炎症状态影响的实验研究
()
分享到：

《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2020年8期
页码:
23-27
栏目:
出版日期:
2020-08-10

文章信息/Info

Title:
 Effects of Infl ammation for Periodontitis Tissue on Different Masticatory Loading
文章编号:
1008-6455（2020）08-0023-04
作者:
 张博伦; 贾 如; 胡 波; 裴丹丹; 刘 洁; 韩如浩; 逯 宜
Author(s):
 ZHANG Bo-lun; JIA Ru; HU Bo; PEI Dan-dan; LIU Jie; HAN Ru-hao; LU Yi
关键词:
 [关键词]牙周炎; 牙列缺损; 力学载荷; 脂多糖; 炎症因子
Keywords:
 Key words: periodontitis;  dentition defect;  masticatory loading;  lipopolysaccharide;  infl ammatory factors
分类号:
R781.4+2
文献标志码:
A
摘要:
[摘要]目的：探讨牙周炎状态下不同咀嚼负荷对牙周组织的炎性状态的影响。方法：取18只大鼠利用局部注射脂多糖
（Lipopolysaccharide，LPS）+高糖饮食喂养方法建立实验性动物牙周炎模型，36d后停止处置并随机分为两组：LPS组与
LPS+soft组各9只，分别用正常饲料与定制软饲料连续喂养7d，另取3只大鼠作为control组。43d后，采集双侧上颌第一磨牙
腭侧龈沟液与牙龈组织及磨牙区上颌骨，分别用于ELISA检测、提取总RNA、反转录后进行Real-time PCR和用于Micro-CT
扫描、三维重建后脱钙进行H&E染色。结果：相比于control组，LPS+soft组和LPS组腭侧牙槽骨均出现明显吸收，炎细胞
浸润，破骨细胞增殖，形成骨吸收陷窝形成，其中LPS组更为明显。LPS组IL-1β、TNF-α在牙龈组织的表达与龈沟液含
量中分泌水平均高于control组，差异具有统计学意义（P ＜0.05）；牙龈组织中IL-6在LPS组的表达也显著高于control
组，差异具有统计学意义（P ＜0.05）；另外TNF-α在LPS组中的基因表达水平较LPS+soft组显著升高，差异具有统计学
意义（P ＜0.05）。结论：不同硬度的食物会导致牙周组织的炎症反应程度出现差异，较软食物相对正常食物有减轻其炎
性状态的作用。

Abstract:
Abstract: Objective To explore the effect of different mechanical loads on the inflammatory state of periodontitis tissue.
Methods 18 rats were taken to establish an experimental periodontitis model by local injection of LPS (Lipopolysaccharides) +
high-sugar feeding method. After 36 days, the injection was stopped and the rats was divided randomly into two groups: the LPS
group and the LPS+soft group, there were 9 rats in each group. The two groups were fed respectively with suffi cient normal
feed and customized soft feed for 7 days. Another 3 rats were taken as control group. After 43 days, the gingival crevicular
fl uid and the gingival tissue of the palatal side of the maxillary fi rst molars were collected for ELISA detection, total RNA
extraction, Real-time PCR after reverse transcription, the maxillary bone of the molar area were collected for H&E staining
after Micro-CT scanning and 3D reconstruction. Results Compared with the control group, the alveolar bone of the LPS+soft
group and the LPS group showed obvious resorption, infl ammatory cell infi ltration, osteoclast proliferation, and Howship’s
lacuna, in particular, the LPS group was more obvious. The expression of IL-1β and TNF-α in gingival tissue and
secretion level of gingival crevicular fluid were higher in the LPS group than in the control group, the differences
were statistically significant (P ＜0.05). The expression of IL-6 in gingival tissue in the LPS group was higher than
that in the control group (P ＜0.05). In addition, the gene expression level of TNF-α in the LPS group was higher than that in
the LPS+soft group (P ＜0.05). Conclusion Different hardness foods will lead to differences in the degree of infl ammation of
periodontal tissues, the softer foods have a role in reducing their infl ammatory status compared to normal diets.

相似文献/References:

[1]丹 丹,杨 磊,贾 慧,等. 骨水平种植体与软组织水平种植体用于牙列缺损修复的对比研究[J].中国美容医学,2018,(12):81.
　DAN Dan,YANG Lei,JIA Hui,et al. Comparative Study of Bone Level Implants and Soft Tissue Level Implants in Repairment of Patients with Dental Defects[J].Medical Aesthetics and Beauty,2018,(8):81.
[2]高志强,王红梅,王玉玮. MagfitEX-600磁性附着体治疗牙列缺损的效果及对患者磁体固位力的影响[J].中国美容医学,2019,(04):103.
　GAO Zhi-qiang,WANG Hong-mei,WANG Yu-wei. Effect of MagfitEX-600 Magnetic Attachment on Dentition Defect and Its Effect on Magnet Retention[J].Medical Aesthetics and Beauty,2019,(8):103.
[3] 田碧媛,张 邯,李俊梅. 口腔正畸治疗牙列缺损的疗效及对牙齿功能的影响[J].中国美容医学,2020,(6):117.
　 TIAN Bi-yuan,ZHANG Han,LI Jun-mei. Curative Efficacy of Orthodontics in Treatment of Dentition Defectand Its EffectsonToothfunction[J].Medical Aesthetics and Beauty,2020,(8):117.
[4] 冉文涛. 骨形成蛋白修饰后的种植体用于牙列缺损种植治疗中的应用探讨[J].中国美容医学,2020,(7):120.
　 RAN Wen-tao. Application of Bone Morphogenetic Protein Modifi ed Implants in Dental Implant Treatment[J].Medical Aesthetics and Beauty,2020,(8):120.
[5] 林素芬,张华贵,熊成梅,等. 口腔种植修复对牙列缺损患者相关功能评分与美观度的影响研究[J].中国美容医学,2020,(10):142.
　 LIN Su-fen,ZHANG Hua-gui,XIONG Cheng-mei,et al. Effect of Dental Implant Restoration on Function Score,Aesthetic Degree andComplications of Patients with Dentition Defect[J].Medical Aesthetics and Beauty,2020,(8):142.
[6] 吕敏敏,于 兰,郭 洁,等. 益生菌片含服对不同程度慢性牙周炎患者牙周PLI、BI、PD、AL水平及美学修复的影响[J].中国美容医学,2020,(11):127.
　 LV Min-min,YU Lan,GUO Jie,et al. Effects of Probiotics Tablets on Periodontal PLI,BI,PD,AL Levels and Aesthetic Repair inPatients with Different Degrees of Chronic Periodontitis[J].Medical Aesthetics and Beauty,2020,(8):127.
[7] 魏谋达,邢劲松,尹晓伟,等. 微创环切技术在牙列缺损种植修复中的应用研究[J].中国美容医学,2020,(12):134.
　 WEI Mou-da,XING Jin-song,YIN Xiao-wei,et al. Study on the Application of Minimally Invasive Circumferential Incision Technique inImplant Restoration of Dentition Defect[J].Medical Aesthetics and Beauty,2020,(8):134.
[8] 何 颖,武明轩,杨冬茹. Er:YAG激光结合牙周翻瓣术治疗重度牙周炎的临床研究[J].中国美容医学,2021,(6):118.
　 HE Ying,WU Ming-xuan,YANG Dong-ru. The Clinical Research of Er: YAG Laser Combined with Periodontal Flap Surgery inPatients with Severe Periodontitis[J].Medical Aesthetics and Beauty,2021,(8):118.
[9]丁 洁,任宏宇,白宇航,等.固定-活动义齿联合修复对牙列缺损患者咀嚼功能和OHIP-14评分及龈沟液炎症因子表达的影响[J].中国美容医学,2022,(3):133.
　DING Jie,REN Hongyu,BAI Yuhang,et al.The Effect of Combined Fixed-removable Denture Restoration on Masticatory Function,OHIP-14 Score and Expression of Inflammatory Factors in Gingival Crevicular Fluid in Patients with Dentition Defect[J].Medical Aesthetics and Beauty,2022,(8):133.
[10]刘静雅,何 军,宋小顺,等.固定-活动义齿联合修复对牙列缺损患者美容效果及咀嚼功能的影响[J].中国美容医学,2022,(3):145.
　LIU Jingya,HE Jun,SONG Xiaoshun,et al.Effect of Fixed-removable Denture Combined Repair on Cosmetic Effect and Masticatory Function of Patients with Dentition Defect[J].Medical Aesthetics and Beauty,2022,(8):145.

更新日期/Last Update: 2020-08-26