[1] 韩伊杨,魏志平,杜佳溪,等. 维生素K3对光老化HaCaT细胞保护作用的研究[J].中国美容医学,2020,(12):102-105.
　 HAN Yi-yang,WEI Zhi-ping,DU Jia-xi,et al. Protective Effect of Vitamin K3 on Photoaged HaCaT Cells[J].Medical Aesthetics and Beauty,2020,(12):102-105.
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 维生素K3对光老化HaCaT细胞保护作用的研究
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2020年12期
页码:
102-105
栏目:
出版日期:
2020-12-10

文章信息/Info

Title:
 Protective Effect of Vitamin K3 on Photoaged HaCaT Cells
文章编号:
1008-6455（2020）12-0102-04
作者:
 韩伊杨1; 魏志平2; 杜佳溪1; 陈 娇1
Author(s):
 HAN Yi-yang1; WEI Zhi-ping2; DU Jia-xi1; CHEN Jiao1
关键词:
 [关键词]HaCaT细胞; 光老化; 紫外线A; 维生素K3; 自噬
Keywords:
 Key words: HaCaT cells;  photoaging;  Ultraviolet A;  Vitamin K3;  autophagy
分类号:
R339.3+8
文献标志码:
A
摘要:
[摘要]目的：探讨维生素K3对UVA辐照下角质形成细胞的光保护作用及相关机制。方法：以5J/cm2的UVA辐照HaCaT细胞建立光
老化细胞模型。采用不同浓度（0、0.125μM、0.25μM、0.5μM）的维生素K3预处理HaCaT细胞24h；CCK-8方法检测细胞增
殖活性；活性氧（ROS）检测荧光探针2’,7’-二氯荧光黄双乙酸盐（DCFH-DA）检测细胞内活性氧水平；Western blot法检测
各组细胞MMP1、COX2、LC3B蛋白的表达。结果：与单纯光照组相比，维生素K3能够促进光老化HaCaT细胞的体外增殖，且呈
浓度依赖性，差异有统计学意义（P ＜0.05）；药物干预组HaCaT细胞的ROS含量降低，差异有统计学意义（P ＜0.05）；细胞
内MMP1、COX2蛋白表达降低，LC3B表达增高，差异有统计学意义（P ＜0.05）。结论：维生素K3能够对UVA辐照HaCaT细胞提
供光保护作用，其机制可能与促进细胞自噬有关。

Abstract:
Abstract: Objective To explore the photoprotective effect of Vit K3 on keratinocytes under UVA irradiation and related
mechanisms. Methods HaCaT cells were irradiated with 5J/cm2 of UVA to establish a photoaging cell model. HaCaT cells
were pretreated with Vit K3 at different concentrations (0, 0.125μM, 0.25μM, 0.5μM) for 24h. Proliferation of cells was detected
by CCK-8 method. Reactive oxygen species (ROS) detection fluorescent probe 2’,7’-dichlorofluorescein yellow diacetate
(DCFH-DA) was used to detect intracellular reactive oxygen level. The expression of MMP1, COX2 and LC3B was detected
by Western blot. Results Compared with the light-only group, Vit K3 could promote the proliferation of HaCaT cells in a
concentration dependent manner, the difference was statistically significant (P ＜0.05). The ROS content of HaCaT cells in the
drug intervention group was lower (P ＜0.05). The expression of MMP1 and COX2 protein was decreased, and the expression
of LC3B was increased (P ＜0.05). Conclusion Vit K3 could provide photoprotection to HaCaT cells irradiated by UVA, and its
mechanism might be related to promoting autophagy.

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更新日期/Last Update: 2021-01-05