[1]石家绮,张丽萍,宋 军,等.小分子化合物I942在UVB照射诱导人黑素细胞树突形成及黑色素合成的促进作用[J].中国美容医学,2023,(6):98-103.
　SHI Jiaqi,ZHANG Liping,SONG Jun,et al.The Small Molecule Compound I942 Promotes the Formation of Dendrites and Melanin Synthesis in Human Melanocytes Induced by UVB Irradiation[J].Medical Aesthetics and Beauty,2023,(6):98-103.
点击复制

小分子化合物I942在UVB照射诱导人黑素细胞树突形成及黑色素合成的促进作用()
分享到：

《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2023年6期
页码:
98-103
栏目:
出版日期:
2023-06-10

文章信息/Info

Title:
The Small Molecule Compound I942 Promotes the Formation of Dendrites and Melanin Synthesis in Human Melanocytes Induced by UVB Irradiation
文章编号:
1008-6455（2023）06-0098-06
作者:
石家绮; 张丽萍; 宋 军; 孙 鸿; 宋丹丹
(江南大学附属医院皮肤科 江苏 无锡 214062 )
Author(s):
SHI Jiaqi; ZHANG Liping; SONG Jun; SUN Hong; SONG Dandan
(Department of Dermatology,Affi liated Hospital of Jiangnan University,Wuxi 214062,Jiangsu,China)
关键词:
小分子化合物I942; 黑素细胞; 树突; 黑色素合成; 白癜风
Keywords:
small molecule compound I942;  melanocyte;  dendrites;  melanogenesis;  vitiligo
分类号:
R758.41
文献标志码:
A
摘要:
目的：探究小分子化合物I942在UVB照射诱导人黑素细胞树突形成及黑色素合成的促进作用。方法：使用浓度为 0 、3.125、6.25、12.5、25、50μmol/L的小分子化合物I942处理正常永生化人表皮黑素细胞系PIG1，采用CCK-8法检 测各组24、48、72 h时的细胞活力。将PIG1细胞分为对照组、UVB组、UVB+2.5μmol/L I942组、UVB+5μmol/L I942组、 UVB+10μmol/L I942 组，按照分组名称进行相应处理。多巴染色观察各组细胞形态，免疫荧光染色观察黑素小体及骨架蛋白 F-actin 分布情况，氢氧化钠裂解法测定黑色素含量，多巴氧化反应法测定酪氨酸酶活性。qRT-PCR、WB检测树突形态相关、 黑色素合成相关mRNA与蛋白表达水平。结果：浓度为0、3.125、6.25、12.5、25、50μmol/L的小分子化合物I942对PIG1细 胞活力无明显影响（P＞0.05）。与对照组相比，UVB组、UVB+2.5 μmol/L I942组、UVB+5 μmol/L I942组、UVB+10μmol/L I942组细胞树突数量、gp100、F-actin、黑色素含量、酪氨酸酶活性、MITF、TYR、TRP-1、TRP-2、Rac1 mRNA与蛋白水平均 升高（P＜0.05），RhoA mRNA 与蛋白水平降低（P＜0.05）。结论：小分子化合物I942结合UVB照射诱导能够增加黑素细胞的 树突数量，提高细胞黑色素含量，提升酪氨酸酶活性，促进黑色素合成。
Abstract:
Objective Explore the eff ect of small molecule compound I942 on the promotion of human melanocyte dendritic formation and melanin synthesis induced by UVB irradiation. Methods Treatment of immortalized human epidermal melanocyte cell line PIG1 cells with the small molecule compound I942 at a concentration of 0, 3.125, 6.25, 12.5, 25, 50 μmol/ L. CCK-8 method to detect cell viability of each group at 24, 48, 72 h. PIG1 cells are divided into control group, UVB group, UVB+2.5 μmol/L I942 group, UVB+5 μmol/L I942 group, UVB+10 μmol/L I942 group. According to the group name for corresponding processing. Dopa staining to observe the morphology of each group of cells. Immunofl uorescence staining to observe the distribution of melanosomes and skeleton protein F-actin. Determination of melanin content by sodium hydroxide pyrolysis method. Determination of tyrosinase activity by DOPA oxidation reaction method. qRT-PCR, WB detection of dendritic morphology-related, melanin synthesis-related mRNA and protein expression levels. Results The small molecule compound I942 at the concentration of 0, 3.125, 6.25, 12.5, 25, 50 μmol/L has no signifi cant eff ect on the viability of PIG1 cells (P ＞0.05). Compared with the control group, the number of cell dendrites, gp100, F-actin, melanin content, tyrosinase activity, MITF, TYR, TRP-1, TRP-2, Rac1 mRNA and protein levels increased in UVB group, UVB+2.5 μmol/L I942 group, UVB+5 μmol/L I942 group, UVB+10 μmol/L I942 group (P＜0.05), and RhoA mRNA and protein levels decreased (P ＜0.05). Conclusion The small molecule compound I942 combined with UVB irradiation can increase the number of dendrites of melanocytes, increase the melanin content of cells, increase tyrosinase activity, and promote melanin synthesis.

相似文献/References:

[1]黄莉明,王 强,方 方. 自体表皮黑素细胞移植联合他卡西醇软膏治疗局限性白癜风临床观察[J].中国美容医学,2019,(03):60.
　HUANG Li-ming,WANG Qiang,FANG Fang. The Clinical Observation of Autologous Epidermal Melanocyte Grafting Combinedwith Tacalcitol Ointment in the Treatment of Localized Vitiligo[J].Medical Aesthetics and Beauty,2019,(6):60.
[2]薛 梅,张德利,刁庆春,等. 丹参酚酸B对过氧化氢引起的黑素细胞凋亡的保护作用研究[J].中国美容医学,2019,(06):64.
　XUE Mei,ZHANG De-li,DIAO Qing-chun,et al. Study on Magnesium Tanshinoate B Alleviated H2O2-induced Apoptosis in Human Epidermal Melanocytes[J].Medical Aesthetics and Beauty,2019,(6):64.
[3]赵莲英,刘冬青,刘文利. 308nm准分子激光联合驱白巴布斯片对面部白癜风患者的美容修复效果研究[J].中国美容医学,2020,(2):60.
　ZHAO Lian-ying,LIU Dong-qing,LIU Wen-li. Study on the Cosmetic Repair Effect in Patients with Facial Vitiligo by Using 308nmExcimer Laser Combined with Qubaibasi Tablets[J].Medical Aesthetics and Beauty,2020,(6):60.
[4] 谷晓广,刁云珍,刘永生,等. 自体培养组织工程表皮移植治疗稳定期白癜风初步临床研究[J].中国美容医学,2021,(4):106.
　 GU Xiao-guang,DIAO Yun-zhen,LIU Yong-sheng,et al. Preliminary Clinical Study of Autologous Culture Tissue Engineering EpidermalGrafting in the Treatment of Stable Vitiligo[J].Medical Aesthetics and Beauty,2021,(6):106.
[5]包树明 综述,诺布央卓 左 蕊 向小燕 审校.microRNA与皮肤衰老的研究进展[J].中国美容医学,2024,(4):186.
　BAO Shuming,NUOBU Yangzhuo,ZUO Rui,et al.Research Progress of microRNA and Skin Aging[J].Medical Aesthetics and Beauty,2024,(6):186.

更新日期/Last Update: 2023-07-10