[1]李志鹏,叶 眉,李 峥,等.富血小板纤维蛋白渗出液对脂肪干细胞成骨分化及纳米羟基磷灰石支架生物相容性的影响[J].中国美容医学,2023,(7):74-77.[doi:10.15909/j.cnki.cn61-1347/r.005777]
　LI Zhipeng,YE Mei,LI Zheng,et al.Effects of Platelet-Rich Fibrin and Nano-hydroxyapatite/Collagen Scaffold on the Differentiaion of Beagle Adipose-derived Stem Cells, ADSCs into Osteoblastsin Invitro[J].Medical Aesthetics and Beauty,2023,(7):74-77.[doi:10.15909/j.cnki.cn61-1347/r.005777]
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富血小板纤维蛋白渗出液对脂肪干细胞成骨分化及纳米羟基磷灰石支架生物相容性的影响()
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《中国美容医学》[ISSN:1008-6445/CN:61-1347/R]

卷:
期数:
2023年7期
页码:
74-77
栏目:
出版日期:
2023-07-10

文章信息/Info

Title:
Effects of Platelet-Rich Fibrin and Nano-hydroxyapatite/Collagen Scaffold on the Differentiaion of Beagle Adipose-derived Stem Cells, ADSCs into Osteoblastsin Invitro
文章编号:
1008-6455（2023）07-0074-04
作者:
李志鹏1 ; 叶 眉1 ; 李 峥1 ; 吴 刚2 ; 张 晨3 ; 王丽雯4 ; 陈 辰4
1.安徽省第二人民医院口腔科 安徽 合肥 230041 ;2.正雅齿科科技(上海)
Author(s):
LI Zhipeng1 ; YE Mei1 ; LI Zheng1 ; WU Gang2 ; ZHANG Chen3 ; WANG Liwen4 ; CHEN Chen4
(1.Department of Stomatology,Anhui No.2 Provincial People’s Hospital,Hefei 230041,Anhui,China; 2.Department of Clinical Medicine,Shanghai Smartee Denti-Technology Co.,Ltd.,Shanghai 201203,China; 3.Department of Stomatology,Anhui Medical College,Hefei 230601,Anhui,China; 4.Department of Pharmacy,the First Affi liated Hospital of USTC,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230036,Anhui,China)
关键词:
富血小板纤维蛋白渗出液; 脂肪干细胞; 成骨分化; 牙周组织工程; 纳米羟基磷灰石
Keywords:
platelet-rich fibrin exudate;  adipose-derived stem cells;  osteoblasts;  periodontal tissue regeneration;  nano-hap/ collagen
分类号:
R318.08
DOI:
10.15909/j.cnki.cn61-1347/r.005777
文献标志码:
A
摘要:
目的：观察Beagle犬脂肪干细胞在向成骨细胞分化过程中富血小板纤维蛋白（Platelet-rich fibrin，PRF）渗出液 的作用，及其与支架材料纳米羟基磷灰石支架生物相容性的实验研究。方法：酶消化法获得Beagle犬脂肪干细胞，将收集 的富血小板纤维蛋白渗出液分为成骨诱导矿化液组(含成骨矿化液+5% PRF渗出液)、B组对照组(含成骨矿化液的10% FBS的 DMEM) 和C组空白组(普通10% FBS的DMEM培养液)，观察PRF渗出液对成骨的影响，并用环境扫描电镜观察Beagle犬脂肪干细胞 与经PRF渗出液孵育的纳米羟基磷灰石支架复合培养后的附着增殖情况。结果：在PRF渗出液的作用下，脂肪干细胞能够更加 有效地向成骨细胞分化，形成更多的钙化结节，结节细小致密。环境扫描电镜观察，脂肪干细胞能在支架上充分地附着伸 展。结论：PRF渗出液能够有效促进脂肪干细胞向成骨细胞分化，纳米羟基磷灰石三维支架材料具有很好的生物相容性。
Abstract:
Objective To investigate the eff ect ofplatelet-rich fi brin extract (PRFe) on the diff entiation of Beagleadiposederi vedstemcellsintoosteoblast, and to study the biocompatibility with nano-hydroxyapatite scaff olds. Methods Beagle ADSCs were obtained by enzyme digestion method. Platelette-rich fibrin exudate collected were divided into osteogenic induced mineralization solution group (containing osteogenic mineralization solution+5% PRFe), control group B (DMEM containing 10% FBS of osteogenic mineralization solution) and blank group C (DMEM medium of normal 10% FBS). The effect of PRF exudate on osteogenesis was observed, and the attachment proliferation of Beagle dog adipose stem cells and nanohydroxyapatite scaff old incubated by PRFe were observed by environmental scanning electron microscopy. Results Under the action of PRF exudate, adipose stem cells can diff erentiate more effi ciently into osteoblasts, more calcifi ed nodules form, and the nodules are small and dense.Environmental scanning electron microscope observation,adipose stem cells can be fully attached and extended on the scaff old. Conclusion PRFe can eff ectively promote the diff erentiation of adipose stem cells into osteoblasts, and nano-hydroxyapatite three-dimensional scaff old material has good biocompatibility.

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更新日期/Last Update: 2023-08-01